Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5201 and GFF5202 are separated by 52 nucleotides GFF5202 and GFF5203 are separated by 165 nucleotides GFF5203 and GFF5204 are separated by 66 nucleotides GFF5204 and GFF5205 are separated by 158 nucleotides
GFF5201 - L-2-keto-3-deoxyarabonate dehydratase (EC 4.2.1.43), at 62,013 to 62,930
GFF5201
GFF5202 - hypothetical protein, at 62,983 to 63,261
GFF5202
GFF5203 - hypothetical protein, at 63,427 to 63,858
GFF5203
GFF5204 - hypothetical protein, at 63,925 to 64,329
GFF5204
GFF5205 - Cyanophycin synthase (EC 6.3.2.29)(EC 6.3.2.30), at 64,488 to 67,055
GFF5205
Position (kb)
63
64 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 62.506 kb on - strand, within GFF5201 at 62.506 kb on - strand, within GFF5201 at 62.515 kb on + strand, within GFF5201 at 62.516 kb on - strand, within GFF5201 at 62.731 kb on - strand, within GFF5201 at 62.731 kb on - strand, within GFF5201 at 62.757 kb on + strand, within GFF5201 at 62.758 kb on - strand, within GFF5201 at 63.068 kb on + strand, within GFF5202 at 63.068 kb on + strand, within GFF5202 at 63.068 kb on + strand, within GFF5202 at 63.068 kb on + strand, within GFF5202 at 63.069 kb on - strand, within GFF5202 at 63.072 kb on - strand, within GFF5202 at 63.125 kb on + strand, within GFF5202 at 63.125 kb on + strand, within GFF5202 at 63.125 kb on + strand, within GFF5202 at 63.126 kb on - strand, within GFF5202 at 63.137 kb on + strand, within GFF5202 at 63.137 kb on + strand, within GFF5202 at 63.138 kb on + strand, within GFF5202 at 63.138 kb on - strand, within GFF5202 at 63.230 kb on - strand, within GFF5202 at 63.230 kb on - strand, within GFF5202 at 63.230 kb on - strand, within GFF5202 at 63.230 kb on - strand, within GFF5202 at 63.280 kb on - strand at 63.297 kb on + strand at 63.297 kb on + strand at 63.297 kb on + strand at 63.297 kb on + strand at 63.297 kb on + strand at 63.297 kb on + strand at 63.297 kb on + strand at 63.297 kb on + strand at 63.297 kb on + strand at 63.297 kb on + strand at 63.298 kb on - strand at 63.298 kb on - strand at 63.298 kb on - strand at 63.298 kb on - strand at 63.298 kb on - strand at 63.298 kb on - strand at 63.298 kb on - strand at 63.298 kb on - strand at 63.298 kb on - strand at 63.299 kb on + strand at 63.299 kb on + strand at 63.299 kb on + strand at 63.299 kb on + strand at 63.299 kb on + strand at 63.299 kb on + strand at 63.299 kb on + strand at 63.300 kb on - strand at 63.300 kb on - strand at 63.300 kb on - strand at 63.300 kb on - strand at 63.300 kb on - strand at 63.300 kb on - strand at 63.300 kb on - strand at 63.303 kb on + strand at 63.303 kb on + strand at 63.303 kb on + strand at 63.303 kb on + strand at 63.303 kb on + strand at 63.303 kb on + strand at 63.303 kb on + strand at 63.303 kb on + strand at 63.303 kb on + strand at 63.303 kb on + strand at 63.304 kb on - strand at 63.304 kb on - strand at 63.304 kb on - strand at 63.304 kb on - strand at 63.304 kb on - strand at 63.304 kb on - strand at 63.304 kb on - strand at 63.304 kb on - strand at 63.304 kb on - strand at 63.321 kb on + strand at 63.347 kb on + strand at 63.347 kb on + strand at 63.758 kb on + strand, within GFF5203 at 63.758 kb on + strand, within GFF5203 at 63.846 kb on + strand at 63.846 kb on + strand at 63.847 kb on - strand at 63.847 kb on - strand at 63.847 kb on - strand at 63.880 kb on + strand at 63.880 kb on + strand at 63.881 kb on - strand at 63.906 kb on - strand at 63.906 kb on - strand at 64.715 kb on - strand at 64.715 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 62,506 - GFF5201 0.54 +1.0 62,506 - GFF5201 0.54 -0.8 62,515 + GFF5201 0.55 +0.5 62,516 - GFF5201 0.55 -0.1 62,731 - GFF5201 0.78 +0.8 62,731 - GFF5201 0.78 +0.0 62,757 + GFF5201 0.81 +0.6 62,758 - GFF5201 0.81 +0.2 63,068 + GFF5202 0.30 -1.2 63,068 + GFF5202 0.30 +0.1 63,068 + GFF5202 0.30 -0.1 63,068 + GFF5202 0.30 -0.8 63,069 - GFF5202 0.31 -0.1 63,072 - GFF5202 0.32 +0.5 63,125 + GFF5202 0.51 +0.2 63,125 + GFF5202 0.51 -0.0 63,125 + GFF5202 0.51 -0.6 63,126 - GFF5202 0.51 -0.0 63,137 + GFF5202 0.55 -1.0 63,137 + GFF5202 0.55 -1.2 63,138 + GFF5202 0.56 -0.2 63,138 - GFF5202 0.56 -0.0 63,230 - GFF5202 0.89 -0.4 63,230 - GFF5202 0.89 -0.2 63,230 - GFF5202 0.89 -1.0 63,230 - GFF5202 0.89 -0.6 63,280 - -1.2 63,297 + +0.6 63,297 + -0.4 63,297 + -0.1 63,297 + +1.6 63,297 + +0.1 63,297 + -0.5 63,297 + -0.1 63,297 + -0.9 63,297 + -1.0 63,297 + -1.8 63,298 - -0.3 63,298 - -0.5 63,298 - -0.2 63,298 - +0.4 63,298 - -0.8 63,298 - -2.1 63,298 - -0.2 63,298 - +0.8 63,298 - -0.8 63,299 + +0.1 63,299 + -0.7 63,299 + +0.7 63,299 + +0.5 63,299 + -0.2 63,299 + -0.2 63,299 + +1.1 63,300 - -0.1 63,300 - +1.0 63,300 - -0.5 63,300 - -0.3 63,300 - -0.1 63,300 - -0.3 63,300 - +0.1 63,303 + -0.7 63,303 + +0.5 63,303 + -0.1 63,303 + -1.2 63,303 + -1.0 63,303 + +0.5 63,303 + -0.1 63,303 + +0.8 63,303 + -1.0 63,303 + +1.9 63,304 - +0.2 63,304 - +2.0 63,304 - +2.0 63,304 - +0.0 63,304 - -0.2 63,304 - -0.8 63,304 - +0.0 63,304 - +1.2 63,304 - -0.2 63,321 + -0.7 63,347 + -0.2 63,347 + +0.1 63,758 + GFF5203 0.77 -0.8 63,758 + GFF5203 0.77 -1.1 63,846 + +0.5 63,846 + -1.3 63,847 - +0.2 63,847 - -0.5 63,847 - +2.0 63,880 + +1.2 63,880 + -0.1 63,881 - +0.4 63,906 - -0.1 63,906 - -0.7 64,715 - -1.0 64,715 - +0.3
Or see this region's nucleotide sequence