Strain Fitness in Variovorax sp. SCN45 around GFF5153

Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
5,031	-				-1.3
5,235	+				-0.4
5,235	+				-0.0
5,236	-				+0.7
5,285	+				-0.9
5,286	-				-0.5
5,286	-				-1.1
5,286	-				-0.7
5,707	+		GFF5153	0.47	-0.2
5,707	+		GFF5153	0.47	+0.3
5,708	-		GFF5153	0.47	-0.5
5,708	-		GFF5153	0.47	-2.0
5,848	+		GFF5153	0.62	-0.2
5,848	+		GFF5153	0.62	-1.7
5,848	+		GFF5153	0.62	-1.0
5,848	+		GFF5153	0.62	-2.3
5,849	-		GFF5153	0.62	-0.7
5,849	-		GFF5153	0.62	-0.4
5,858	+		GFF5153	0.63	-1.9
5,859	-		GFF5153	0.63	+0.5
5,859	-		GFF5153	0.63	+1.1
5,859	-		GFF5153	0.63	-0.8
5,859	-		GFF5153	0.63	-1.0
5,859	-		GFF5153	0.63	-1.7
5,998	+		GFF5153	0.78	-0.6
5,998	+		GFF5153	0.78	-1.3
5,998	+		GFF5153	0.78	-1.2
5,998	+		GFF5153	0.78	+1.5
5,998	+		GFF5153	0.78	-1.8
5,998	+		GFF5153	0.78	-2.0
5,998	+		GFF5153	0.78	+0.2
5,998	+		GFF5153	0.78	-1.0
5,998	+		GFF5153	0.78	-1.3
5,998	+		GFF5153	0.78	-2.2
5,998	+		GFF5153	0.78	-2.1
5,998	+		GFF5153	0.78	-1.5
5,998	+		GFF5153	0.78	+1.8
5,998	+		GFF5153	0.78	-1.0
5,999	-		GFF5153	0.78	+1.1
5,999	-		GFF5153	0.78	-0.5
5,999	-		GFF5153	0.78	-0.2
5,999	-		GFF5153	0.78	-0.6
5,999	-		GFF5153	0.78	-0.0
5,999	-		GFF5153	0.78	-1.4
5,999	-		GFF5153	0.78	-0.7
6,172	+				-0.9
6,173	-				-1.5
6,173	-				+0.2
6,173	-				-1.0
6,311	-				-0.1
6,337	+		GFF5154	0.11	-0.9
6,337	+		GFF5154	0.11	+0.6
6,337	+		GFF5154	0.11	-1.2
6,337	+		GFF5154	0.11	-0.2
6,337	+		GFF5154	0.11	-0.3
6,338	-		GFF5154	0.11	-1.0
6,338	-		GFF5154	0.11	-0.2
6,489	-		GFF5154	0.21	-0.7
6,503	-		GFF5154	0.22	-1.0
6,578	-		GFF5154	0.26	-0.2
6,578	-		GFF5154	0.26	+0.6
6,578	-		GFF5154	0.26	+0.1
6,578	-		GFF5154	0.26	-0.5
6,578	-		GFF5154	0.26	-0.3
6,646	+		GFF5154	0.31	+0.0
6,646	+		GFF5154	0.31	+0.2
6,646	+		GFF5154	0.31	+0.3
6,646	+		GFF5154	0.31	-0.1
6,646	+		GFF5154	0.31	+1.3
6,646	+		GFF5154	0.31	-0.8
6,647	-		GFF5154	0.31	+0.6
6,647	-		GFF5154	0.31	+1.8
6,647	-		GFF5154	0.31	+0.2
6,647	-		GFF5154	0.31	-0.6
6,739	+		GFF5154	0.37	+0.1
6,739	+		GFF5154	0.37	+1.4
6,739	+		GFF5154	0.37	-0.4
6,739	+		GFF5154	0.37	-0.8
6,739	+		GFF5154	0.37	-1.9
6,739	+		GFF5154	0.37	-1.2
6,739	+		GFF5154	0.37	-0.8
6,740	-		GFF5154	0.37	-0.0
6,740	-		GFF5154	0.37	-2.3
6,740	-		GFF5154	0.37	+1.5
6,740	-		GFF5154	0.37	-0.9
6,740	-		GFF5154	0.37	+0.4
6,740	-		GFF5154	0.37	-0.7
6,811	+		GFF5154	0.42	+0.6
6,811	+		GFF5154	0.42	-0.4
6,811	+		GFF5154	0.42	+2.3
6,811	+		GFF5154	0.42	-0.5
6,811	+		GFF5154	0.42	+0.2
6,811	+		GFF5154	0.42	+2.1
6,812	-		GFF5154	0.42	-0.1
6,812	-		GFF5154	0.42	+0.7
6,812	-		GFF5154	0.42	-1.1
6,812	-		GFF5154	0.42	-1.2
6,946	+		GFF5154	0.50	+0.0
6,946	+		GFF5154	0.50	-0.9
6,946	+		GFF5154	0.50	-1.5
6,946	+		GFF5154	0.50	+0.2
6,946	+		GFF5154	0.50	-2.0
6,946	+		GFF5154	0.50	-0.9
6,946	+		GFF5154	0.50	+0.3
6,946	+		GFF5154	0.50	-0.8
6,947	-		GFF5154	0.50	-1.6
6,947	-		GFF5154	0.50	-0.6
6,947	-		GFF5154	0.50	-1.5
6,947	-		GFF5154	0.50	-0.2
7,005	+		GFF5154	0.54	-0.6
7,005	+		GFF5154	0.54	-0.4
7,006	-		GFF5154	0.54	-1.1
7,054	+		GFF5154	0.57	-0.7
7,054	+		GFF5154	0.57	+0.1
7,054	+		GFF5154	0.57	-0.5
7,054	+		GFF5154	0.57	+1.0
7,054	+		GFF5154	0.57	-1.5
7,054	+		GFF5154	0.57	+0.1
7,054	+		GFF5154	0.57	+1.0
7,054	+		GFF5154	0.57	+0.1
7,055	-		GFF5154	0.57	-0.6
7,055	-		GFF5154	0.57	+0.3
7,055	-		GFF5154	0.57	+1.0
7,055	-		GFF5154	0.57	+0.1