Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5042 and GFF5043 are separated by 182 nucleotides GFF5043 and GFF5044 are separated by 4 nucleotides GFF5044 and GFF5045 are separated by 16 nucleotides
GFF5042 - Transcriptional regulator, LysR family, at 143,817 to 144,749
GFF5042
GFF5043 - ABC transporter, permease protein (cluster 9, phospholipid), at 144,932 to 146,098
GFF5043
GFF5044 - ABC transporter, ATP-binding protein (cluster 9, phospholipid), at 146,103 to 146,948
GFF5044
GFF5045 - ABC transporter, substrate-binding protein (cluster 9, phospholipid), at 146,965 to 147,924
GFF5045
Position (kb)
144
145
146
147 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 144.345 kb on + strand, within GFF5042 at 144.345 kb on + strand, within GFF5042 at 144.346 kb on - strand, within GFF5042 at 144.346 kb on - strand, within GFF5042 at 144.654 kb on + strand, within GFF5042 at 144.654 kb on + strand, within GFF5042 at 144.654 kb on + strand, within GFF5042 at 144.669 kb on + strand at 144.670 kb on - strand at 144.670 kb on - strand at 144.755 kb on + strand at 144.755 kb on + strand at 144.755 kb on + strand at 144.756 kb on - strand at 144.756 kb on - strand at 144.801 kb on - strand at 144.832 kb on - strand at 144.859 kb on + strand at 144.859 kb on + strand at 144.859 kb on + strand at 144.860 kb on - strand at 144.860 kb on - strand at 144.878 kb on + strand at 144.879 kb on - strand at 144.911 kb on - strand at 144.911 kb on - strand at 144.911 kb on - strand at 144.931 kb on + strand at 144.931 kb on + strand at 144.931 kb on + strand at 144.931 kb on + strand at 144.931 kb on + strand at 144.931 kb on + strand at 144.931 kb on + strand at 144.931 kb on + strand at 144.931 kb on + strand at 144.931 kb on + strand at 144.931 kb on + strand at 144.931 kb on + strand at 144.932 kb on - strand at 144.932 kb on - strand at 144.932 kb on - strand at 144.932 kb on - strand at 144.932 kb on - strand at 145.259 kb on + strand, within GFF5043 at 145.259 kb on + strand, within GFF5043 at 145.259 kb on + strand, within GFF5043 at 145.259 kb on + strand, within GFF5043 at 145.259 kb on + strand, within GFF5043 at 145.259 kb on + strand, within GFF5043 at 145.259 kb on + strand, within GFF5043 at 145.259 kb on + strand, within GFF5043 at 145.259 kb on + strand, within GFF5043 at 145.260 kb on - strand, within GFF5043 at 145.260 kb on - strand, within GFF5043 at 145.260 kb on - strand, within GFF5043 at 145.260 kb on - strand, within GFF5043 at 145.260 kb on - strand, within GFF5043 at 145.260 kb on - strand, within GFF5043 at 145.451 kb on + strand, within GFF5043 at 145.452 kb on - strand, within GFF5043 at 145.452 kb on - strand, within GFF5043 at 145.452 kb on - strand, within GFF5043 at 145.497 kb on + strand, within GFF5043 at 145.498 kb on - strand, within GFF5043 at 145.498 kb on - strand, within GFF5043 at 145.520 kb on + strand, within GFF5043 at 145.521 kb on - strand, within GFF5043 at 145.521 kb on - strand, within GFF5043 at 145.521 kb on - strand, within GFF5043 at 145.521 kb on - strand, within GFF5043 at 145.716 kb on + strand, within GFF5043 at 145.716 kb on + strand, within GFF5043 at 145.843 kb on + strand, within GFF5043 at 145.843 kb on + strand, within GFF5043 at 145.843 kb on + strand, within GFF5043 at 145.843 kb on + strand, within GFF5043 at 145.843 kb on + strand, within GFF5043 at 145.843 kb on + strand, within GFF5043 at 145.844 kb on - strand, within GFF5043 at 145.844 kb on - strand, within GFF5043 at 145.856 kb on + strand, within GFF5043 at 145.888 kb on + strand, within GFF5043 at 145.888 kb on + strand, within GFF5043 at 145.888 kb on + strand, within GFF5043 at 145.888 kb on + strand, within GFF5043 at 145.889 kb on - strand, within GFF5043 at 145.889 kb on - strand, within GFF5043 at 145.889 kb on - strand, within GFF5043 at 145.958 kb on + strand, within GFF5043 at 145.958 kb on + strand, within GFF5043 at 145.958 kb on + strand, within GFF5043 at 145.958 kb on + strand, within GFF5043 at 145.959 kb on - strand, within GFF5043 at 145.959 kb on - strand, within GFF5043 at 145.959 kb on - strand, within GFF5043 at 145.959 kb on - strand, within GFF5043 at 145.959 kb on - strand, within GFF5043 at 145.959 kb on - strand, within GFF5043 at 146.029 kb on + strand at 146.029 kb on + strand at 146.030 kb on - strand at 146.030 kb on - strand at 146.030 kb on - strand at 146.030 kb on - strand at 146.847 kb on + strand, within GFF5044 at 146.847 kb on + strand, within GFF5044 at 146.847 kb on + strand, within GFF5044 at 146.847 kb on + strand, within GFF5044 at 146.848 kb on - strand, within GFF5044 at 146.848 kb on - strand, within GFF5044 at 146.953 kb on + strand at 146.953 kb on + strand at 146.953 kb on + strand at 146.953 kb on + strand at 146.953 kb on + strand at 146.953 kb on + strand at 146.954 kb on - strand at 146.954 kb on - strand at 146.957 kb on + strand at 146.957 kb on + strand at 146.957 kb on + strand at 146.957 kb on + strand at 146.957 kb on + strand at 146.958 kb on - strand at 146.958 kb on - strand at 146.958 kb on - strand at 146.958 kb on - strand at 146.958 kb on - strand at 146.958 kb on - strand at 146.958 kb on - strand at 146.958 kb on - strand at 146.958 kb on - strand at 146.969 kb on - strand at 147.076 kb on + strand, within GFF5045
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 144,345 + GFF5042 0.57 +0.2 144,345 + GFF5042 0.57 -1.4 144,346 - GFF5042 0.57 -0.7 144,346 - GFF5042 0.57 +1.2 144,654 + GFF5042 0.90 -0.5 144,654 + GFF5042 0.90 -1.9 144,654 + GFF5042 0.90 -1.7 144,669 + +0.1 144,670 - -0.3 144,670 - -0.7 144,755 + -0.4 144,755 + -0.8 144,755 + -0.2 144,756 - +1.1 144,756 - -0.3 144,801 - -0.9 144,832 - -1.1 144,859 + +0.3 144,859 + -0.9 144,859 + -2.1 144,860 - -1.1 144,860 - -0.6 144,878 + +0.1 144,879 - -0.1 144,911 - -1.6 144,911 - -0.7 144,911 - -0.0 144,931 + -2.2 144,931 + -0.7 144,931 + -0.4 144,931 + -0.2 144,931 + -0.2 144,931 + -0.3 144,931 + -0.3 144,931 + -0.1 144,931 + +0.0 144,931 + -0.0 144,931 + -0.3 144,931 + -0.3 144,932 - -0.4 144,932 - -0.5 144,932 - -0.0 144,932 - -0.0 144,932 - -1.8 145,259 + GFF5043 0.28 -0.1 145,259 + GFF5043 0.28 +0.2 145,259 + GFF5043 0.28 +0.7 145,259 + GFF5043 0.28 +1.0 145,259 + GFF5043 0.28 -0.8 145,259 + GFF5043 0.28 -0.4 145,259 + GFF5043 0.28 -0.4 145,259 + GFF5043 0.28 -1.1 145,259 + GFF5043 0.28 +0.8 145,260 - GFF5043 0.28 -0.5 145,260 - GFF5043 0.28 -1.7 145,260 - GFF5043 0.28 +0.1 145,260 - GFF5043 0.28 -0.7 145,260 - GFF5043 0.28 -0.3 145,260 - GFF5043 0.28 -0.3 145,451 + GFF5043 0.44 -0.6 145,452 - GFF5043 0.45 -0.6 145,452 - GFF5043 0.45 +1.2 145,452 - GFF5043 0.45 -1.1 145,497 + GFF5043 0.48 -0.0 145,498 - GFF5043 0.49 -0.3 145,498 - GFF5043 0.49 -0.5 145,520 + GFF5043 0.50 +0.4 145,521 - GFF5043 0.50 -1.1 145,521 - GFF5043 0.50 -0.8 145,521 - GFF5043 0.50 -0.2 145,521 - GFF5043 0.50 -0.5 145,716 + GFF5043 0.67 -1.1 145,716 + GFF5043 0.67 -1.1 145,843 + GFF5043 0.78 +0.2 145,843 + GFF5043 0.78 -0.9 145,843 + GFF5043 0.78 -1.0 145,843 + GFF5043 0.78 -2.0 145,843 + GFF5043 0.78 +0.1 145,843 + GFF5043 0.78 -0.5 145,844 - GFF5043 0.78 +0.9 145,844 - GFF5043 0.78 -0.4 145,856 + GFF5043 0.79 +0.1 145,888 + GFF5043 0.82 -0.4 145,888 + GFF5043 0.82 -1.1 145,888 + GFF5043 0.82 -1.1 145,888 + GFF5043 0.82 -1.3 145,889 - GFF5043 0.82 +0.2 145,889 - GFF5043 0.82 +0.9 145,889 - GFF5043 0.82 -2.2 145,958 + GFF5043 0.88 -0.9 145,958 + GFF5043 0.88 -0.7 145,958 + GFF5043 0.88 -0.0 145,958 + GFF5043 0.88 -0.8 145,959 - GFF5043 0.88 +0.1 145,959 - GFF5043 0.88 -0.3 145,959 - GFF5043 0.88 -0.6 145,959 - GFF5043 0.88 -0.3 145,959 - GFF5043 0.88 -0.1 145,959 - GFF5043 0.88 -0.3 146,029 + -1.5 146,029 + -0.1 146,030 - -0.9 146,030 - -0.3 146,030 - +1.1 146,030 - +0.5 146,847 + GFF5044 0.88 -0.6 146,847 + GFF5044 0.88 -1.0 146,847 + GFF5044 0.88 -0.0 146,847 + GFF5044 0.88 +0.6 146,848 - GFF5044 0.88 +1.1 146,848 - GFF5044 0.88 +0.3 146,953 + -0.4 146,953 + +0.5 146,953 + -1.1 146,953 + +0.7 146,953 + -0.1 146,953 + +0.2 146,954 - +1.9 146,954 - +1.2 146,957 + -1.7 146,957 + -1.9 146,957 + +0.9 146,957 + -0.1 146,957 + -1.8 146,958 - -0.3 146,958 - +0.6 146,958 - -0.1 146,958 - -0.7 146,958 - -0.4 146,958 - +0.8 146,958 - +0.3 146,958 - -0.7 146,958 - -0.5 146,969 - +1.0 147,076 + GFF5045 0.12 -0.1
Or see this region's nucleotide sequence