Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF500 and GFF501 are separated by 119 nucleotides GFF501 and GFF502 are separated by 4 nucleotides GFF502 and GFF503 are separated by 17 nucleotides
GFF500 - Lysine exporter protein (LYSE/YGGA), at 543,700 to 544,344
GFF500
GFF501 - hypothetical protein, at 544,464 to 544,769
GFF501
GFF502 - Hydrolase, at 544,774 to 545,505
GFF502
GFF503 - Ferrochelatase, protoheme ferro-lyase (EC 4.99.1.1), at 545,523 to 546,554
GFF503
Position (kb) 544
545
546 Strain fitness (log2 ratio) -2
-1
0
1 at 543.836 kb on + strand, within GFF500 at 543.836 kb on + strand, within GFF500 at 543.836 kb on + strand, within GFF500 at 543.836 kb on + strand, within GFF500 at 543.837 kb on - strand, within GFF500 at 543.837 kb on - strand, within GFF500 at 543.837 kb on - strand, within GFF500 at 543.837 kb on - strand, within GFF500 at 543.837 kb on - strand, within GFF500 at 544.091 kb on + strand, within GFF500 at 544.091 kb on + strand, within GFF500 at 544.091 kb on + strand, within GFF500 at 544.091 kb on + strand, within GFF500 at 544.092 kb on - strand, within GFF500 at 544.092 kb on - strand, within GFF500 at 544.092 kb on - strand, within GFF500 at 544.092 kb on - strand, within GFF500 at 544.092 kb on - strand, within GFF500 at 544.100 kb on + strand, within GFF500 at 544.101 kb on - strand, within GFF500 at 544.101 kb on - strand, within GFF500 at 544.101 kb on - strand, within GFF500 at 544.397 kb on + strand at 544.397 kb on + strand at 544.397 kb on + strand at 544.398 kb on - strand at 544.398 kb on - strand at 544.398 kb on - strand at 544.398 kb on - strand at 544.398 kb on - strand at 544.398 kb on - strand at 544.398 kb on - strand at 544.399 kb on + strand at 544.400 kb on - strand at 544.437 kb on - strand at 544.477 kb on - strand at 544.477 kb on - strand at 544.477 kb on - strand at 544.477 kb on - strand at 544.485 kb on + strand at 544.485 kb on + strand at 544.485 kb on + strand at 544.485 kb on + strand at 544.485 kb on + strand at 544.485 kb on + strand at 544.486 kb on - strand at 544.486 kb on - strand at 544.486 kb on - strand at 544.486 kb on - strand at 544.486 kb on - strand at 544.486 kb on - strand at 544.500 kb on + strand, within GFF501 at 544.500 kb on + strand, within GFF501 at 544.501 kb on - strand, within GFF501 at 544.501 kb on - strand, within GFF501 at 544.578 kb on + strand, within GFF501 at 544.578 kb on + strand, within GFF501 at 544.578 kb on + strand, within GFF501 at 544.579 kb on - strand, within GFF501 at 544.704 kb on + strand, within GFF501 at 544.734 kb on + strand, within GFF501 at 544.734 kb on + strand, within GFF501 at 544.735 kb on - strand, within GFF501 at 544.735 kb on - strand, within GFF501 at 544.747 kb on - strand at 544.767 kb on + strand at 544.768 kb on - strand at 544.768 kb on - strand at 544.768 kb on - strand at 544.768 kb on - strand at 544.768 kb on - strand at 544.768 kb on - strand at 544.768 kb on - strand at 544.768 kb on - strand at 544.768 kb on - strand at 545.141 kb on + strand, within GFF502 at 545.141 kb on + strand, within GFF502 at 545.517 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 543,836 + GFF500 0.21 -0.4 543,836 + GFF500 0.21 -1.5 543,836 + GFF500 0.21 -0.9 543,836 + GFF500 0.21 +0.8 543,837 - GFF500 0.21 -0.1 543,837 - GFF500 0.21 -0.4 543,837 - GFF500 0.21 +0.1 543,837 - GFF500 0.21 +0.4 543,837 - GFF500 0.21 -0.2 544,091 + GFF500 0.61 -1.4 544,091 + GFF500 0.61 -0.1 544,091 + GFF500 0.61 -0.6 544,091 + GFF500 0.61 -0.2 544,092 - GFF500 0.61 -0.8 544,092 - GFF500 0.61 -0.6 544,092 - GFF500 0.61 -1.0 544,092 - GFF500 0.61 -0.0 544,092 - GFF500 0.61 -0.6 544,100 + GFF500 0.62 +0.3 544,101 - GFF500 0.62 +0.2 544,101 - GFF500 0.62 -0.6 544,101 - GFF500 0.62 -0.9 544,397 + -0.4 544,397 + -0.2 544,397 + -0.6 544,398 - +0.0 544,398 - +0.8 544,398 - -0.3 544,398 - -0.1 544,398 - -0.2 544,398 - +0.7 544,398 - +0.6 544,399 + -1.8 544,400 - +0.1 544,437 - -0.9 544,477 - -0.2 544,477 - +0.0 544,477 - -0.8 544,477 - -0.8 544,485 + +0.4 544,485 + -0.0 544,485 + -0.4 544,485 + -0.1 544,485 + +0.1 544,485 + -0.1 544,486 - +0.5 544,486 - -0.5 544,486 - -0.1 544,486 - -0.1 544,486 - -0.4 544,486 - -0.4 544,500 + GFF501 0.12 +1.0 544,500 + GFF501 0.12 +0.0 544,501 - GFF501 0.12 +0.3 544,501 - GFF501 0.12 -0.5 544,578 + GFF501 0.37 -0.2 544,578 + GFF501 0.37 +0.4 544,578 + GFF501 0.37 -0.1 544,579 - GFF501 0.38 +0.7 544,704 + GFF501 0.78 -1.8 544,734 + GFF501 0.88 +0.8 544,734 + GFF501 0.88 +0.5 544,735 - GFF501 0.89 -0.2 544,735 - GFF501 0.89 -1.6 544,747 - -0.1 544,767 + +1.7 544,768 - +0.8 544,768 - +0.3 544,768 - -0.7 544,768 - -1.4 544,768 - -0.9 544,768 - +1.7 544,768 - +0.3 544,768 - -1.9 544,768 - +0.2 545,141 + GFF502 0.50 +0.6 545,141 + GFF502 0.50 -1.6 545,517 + -0.8
Or see this region's nucleotide sequence