Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4715 and GFF4716 are separated by 147 nucleotides GFF4716 and GFF4717 are separated by 10 nucleotides GFF4717 and GFF4718 are separated by 1 nucleotides
GFF4715 - Fatty acid hydroxylase family (carotene hydroxylase/sterol desaturase), at 77,271 to 78,401
GFF4715
GFF4716 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 78,549 to 79,382
GFF4716
GFF4717 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 79,393 to 80,190
GFF4717
GFF4718 - ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines), at 80,192 to 80,962
GFF4718
Position (kb) 78
79
80 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 77.560 kb on + strand, within GFF4715 at 77.560 kb on + strand, within GFF4715 at 77.560 kb on + strand, within GFF4715 at 77.560 kb on + strand, within GFF4715 at 77.560 kb on + strand, within GFF4715 at 77.560 kb on + strand, within GFF4715 at 77.560 kb on + strand, within GFF4715 at 77.560 kb on + strand, within GFF4715 at 77.560 kb on + strand, within GFF4715 at 77.561 kb on - strand, within GFF4715 at 77.596 kb on + strand, within GFF4715 at 77.596 kb on + strand, within GFF4715 at 77.596 kb on + strand, within GFF4715 at 77.596 kb on + strand, within GFF4715 at 77.596 kb on + strand, within GFF4715 at 77.596 kb on + strand, within GFF4715 at 77.596 kb on + strand, within GFF4715 at 77.596 kb on + strand, within GFF4715 at 77.597 kb on - strand, within GFF4715 at 77.597 kb on - strand, within GFF4715 at 77.597 kb on - strand, within GFF4715 at 77.597 kb on - strand, within GFF4715 at 77.597 kb on - strand, within GFF4715 at 77.597 kb on - strand, within GFF4715 at 77.597 kb on - strand, within GFF4715 at 77.597 kb on - strand, within GFF4715 at 77.597 kb on - strand, within GFF4715 at 77.597 kb on - strand, within GFF4715 at 77.597 kb on - strand, within GFF4715 at 77.728 kb on + strand, within GFF4715 at 77.728 kb on + strand, within GFF4715 at 77.729 kb on - strand, within GFF4715 at 77.729 kb on - strand, within GFF4715 at 77.746 kb on + strand, within GFF4715 at 77.746 kb on + strand, within GFF4715 at 77.747 kb on - strand, within GFF4715 at 77.747 kb on - strand, within GFF4715 at 77.747 kb on - strand, within GFF4715 at 77.747 kb on - strand, within GFF4715 at 78.043 kb on + strand, within GFF4715 at 78.043 kb on + strand, within GFF4715 at 78.043 kb on + strand, within GFF4715 at 78.043 kb on + strand, within GFF4715 at 78.043 kb on + strand, within GFF4715 at 78.208 kb on + strand, within GFF4715 at 78.208 kb on + strand, within GFF4715 at 78.208 kb on + strand, within GFF4715 at 78.208 kb on + strand, within GFF4715 at 78.209 kb on - strand, within GFF4715 at 78.209 kb on - strand, within GFF4715 at 78.209 kb on - strand, within GFF4715 at 78.209 kb on - strand, within GFF4715 at 78.209 kb on - strand, within GFF4715 at 78.209 kb on - strand, within GFF4715 at 78.209 kb on - strand, within GFF4715 at 78.209 kb on - strand, within GFF4715 at 78.229 kb on + strand, within GFF4715 at 78.229 kb on + strand, within GFF4715 at 78.229 kb on + strand, within GFF4715 at 78.230 kb on - strand, within GFF4715 at 78.230 kb on - strand, within GFF4715 at 78.230 kb on - strand, within GFF4715 at 78.230 kb on - strand, within GFF4715 at 78.230 kb on - strand, within GFF4715 at 78.230 kb on - strand, within GFF4715 at 78.259 kb on + strand, within GFF4715 at 78.260 kb on - strand, within GFF4715 at 78.260 kb on - strand, within GFF4715 at 78.260 kb on - strand, within GFF4715 at 78.260 kb on - strand, within GFF4715 at 78.260 kb on - strand, within GFF4715 at 78.447 kb on - strand at 78.510 kb on + strand at 78.511 kb on - strand at 78.511 kb on - strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.520 kb on + strand at 78.521 kb on - strand at 78.531 kb on + strand at 78.531 kb on + strand at 78.531 kb on + strand at 78.531 kb on + strand at 78.532 kb on - strand at 78.532 kb on - strand at 78.702 kb on + strand, within GFF4716 at 78.795 kb on + strand, within GFF4716 at 78.795 kb on + strand, within GFF4716 at 78.795 kb on + strand, within GFF4716 at 78.795 kb on + strand, within GFF4716 at 78.796 kb on - strand, within GFF4716 at 78.796 kb on - strand, within GFF4716 at 78.927 kb on + strand, within GFF4716 at 78.927 kb on + strand, within GFF4716 at 78.927 kb on + strand, within GFF4716 at 78.927 kb on + strand, within GFF4716 at 78.927 kb on + strand, within GFF4716 at 78.927 kb on + strand, within GFF4716 at 78.927 kb on + strand, within GFF4716 at 78.927 kb on + strand, within GFF4716 at 78.927 kb on + strand, within GFF4716 at 78.927 kb on + strand, within GFF4716 at 78.928 kb on - strand, within GFF4716 at 78.928 kb on - strand, within GFF4716 at 78.928 kb on - strand, within GFF4716 at 78.928 kb on - strand, within GFF4716 at 78.928 kb on - strand, within GFF4716 at 78.928 kb on - strand, within GFF4716 at 78.928 kb on - strand, within GFF4716 at 78.928 kb on - strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.203 kb on + strand, within GFF4716 at 79.525 kb on + strand, within GFF4717 at 79.526 kb on - strand, within GFF4717 at 79.585 kb on + strand, within GFF4717 at 79.585 kb on + strand, within GFF4717 at 79.586 kb on - strand, within GFF4717 at 79.696 kb on + strand, within GFF4717 at 79.696 kb on + strand, within GFF4717 at 79.696 kb on + strand, within GFF4717 at 79.696 kb on + strand, within GFF4717 at 79.696 kb on + strand, within GFF4717 at 79.696 kb on + strand, within GFF4717 at 79.696 kb on + strand, within GFF4717 at 79.697 kb on - strand, within GFF4717 at 79.697 kb on - strand, within GFF4717 at 79.697 kb on - strand, within GFF4717 at 79.747 kb on + strand, within GFF4717 at 79.747 kb on + strand, within GFF4717 at 79.747 kb on + strand, within GFF4717 at 79.747 kb on + strand, within GFF4717 at 79.747 kb on + strand, within GFF4717 at 79.747 kb on + strand, within GFF4717 at 79.748 kb on - strand, within GFF4717 at 79.748 kb on - strand, within GFF4717 at 79.748 kb on - strand, within GFF4717 at 79.748 kb on - strand, within GFF4717 at 79.750 kb on + strand, within GFF4717 at 79.819 kb on + strand, within GFF4717 at 79.834 kb on + strand, within GFF4717 at 80.044 kb on + strand, within GFF4717 at 80.045 kb on - strand, within GFF4717 at 80.045 kb on - strand, within GFF4717 at 80.074 kb on + strand, within GFF4717 at 80.074 kb on + strand, within GFF4717 at 80.075 kb on - strand, within GFF4717 at 80.075 kb on - strand, within GFF4717 at 80.075 kb on - strand, within GFF4717 at 80.075 kb on - strand, within GFF4717 at 80.075 kb on - strand, within GFF4717 at 80.093 kb on - strand, within GFF4717 at 80.093 kb on - strand, within GFF4717 at 80.093 kb on - strand, within GFF4717 at 80.113 kb on + strand at 80.114 kb on - strand at 80.114 kb on - strand at 80.114 kb on - strand at 80.114 kb on - strand at 80.114 kb on - strand at 80.179 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 77,560 + GFF4715 0.26 -1.5 77,560 + GFF4715 0.26 +0.9 77,560 + GFF4715 0.26 -0.8 77,560 + GFF4715 0.26 +0.7 77,560 + GFF4715 0.26 -0.9 77,560 + GFF4715 0.26 +0.6 77,560 + GFF4715 0.26 -0.6 77,560 + GFF4715 0.26 +1.5 77,560 + GFF4715 0.26 -0.2 77,561 - GFF4715 0.26 +1.1 77,596 + GFF4715 0.29 +0.2 77,596 + GFF4715 0.29 -0.6 77,596 + GFF4715 0.29 -0.2 77,596 + GFF4715 0.29 +0.9 77,596 + GFF4715 0.29 -0.5 77,596 + GFF4715 0.29 +0.6 77,596 + GFF4715 0.29 -1.3 77,596 + GFF4715 0.29 -0.1 77,597 - GFF4715 0.29 +0.9 77,597 - GFF4715 0.29 +0.7 77,597 - GFF4715 0.29 -0.1 77,597 - GFF4715 0.29 -0.2 77,597 - GFF4715 0.29 -0.3 77,597 - GFF4715 0.29 -0.1 77,597 - GFF4715 0.29 +0.2 77,597 - GFF4715 0.29 -0.2 77,597 - GFF4715 0.29 +0.7 77,597 - GFF4715 0.29 -1.2 77,597 - GFF4715 0.29 -0.4 77,728 + GFF4715 0.40 -0.0 77,728 + GFF4715 0.40 -0.4 77,729 - GFF4715 0.40 -2.5 77,729 - GFF4715 0.40 -0.3 77,746 + GFF4715 0.42 -0.3 77,746 + GFF4715 0.42 -0.3 77,747 - GFF4715 0.42 +0.6 77,747 - GFF4715 0.42 -1.0 77,747 - GFF4715 0.42 +0.2 77,747 - GFF4715 0.42 -0.1 78,043 + GFF4715 0.68 +0.3 78,043 + GFF4715 0.68 -0.1 78,043 + GFF4715 0.68 -0.9 78,043 + GFF4715 0.68 +0.2 78,043 + GFF4715 0.68 -0.8 78,208 + GFF4715 0.83 +1.1 78,208 + GFF4715 0.83 +1.2 78,208 + GFF4715 0.83 -0.6 78,208 + GFF4715 0.83 +0.5 78,209 - GFF4715 0.83 +0.6 78,209 - GFF4715 0.83 +0.1 78,209 - GFF4715 0.83 -0.8 78,209 - GFF4715 0.83 -0.9 78,209 - GFF4715 0.83 -0.4 78,209 - GFF4715 0.83 -1.0 78,209 - GFF4715 0.83 -0.8 78,209 - GFF4715 0.83 +0.6 78,229 + GFF4715 0.85 -0.1 78,229 + GFF4715 0.85 -0.9 78,229 + GFF4715 0.85 +0.0 78,230 - GFF4715 0.85 -0.6 78,230 - GFF4715 0.85 +0.4 78,230 - GFF4715 0.85 -1.1 78,230 - GFF4715 0.85 -0.5 78,230 - GFF4715 0.85 -0.5 78,230 - GFF4715 0.85 +0.1 78,259 + GFF4715 0.87 +0.3 78,260 - GFF4715 0.87 -0.4 78,260 - GFF4715 0.87 -0.5 78,260 - GFF4715 0.87 +0.7 78,260 - GFF4715 0.87 -0.5 78,260 - GFF4715 0.87 -1.5 78,447 - -0.4 78,510 + +0.9 78,511 - -0.2 78,511 - +0.1 78,518 + -0.2 78,518 + -0.5 78,518 + +0.2 78,518 + -0.7 78,518 + -0.4 78,518 + +1.1 78,518 + -0.5 78,518 + -0.9 78,518 + -1.0 78,518 + +0.7 78,518 + -0.4 78,518 + -0.9 78,518 + +0.4 78,518 + +0.2 78,518 + -0.7 78,518 + -0.5 78,519 - -0.8 78,519 - -0.6 78,519 - -0.4 78,519 - +0.2 78,519 - -2.1 78,519 - +0.0 78,519 - -0.9 78,519 - +0.6 78,519 - +0.9 78,519 - -0.7 78,519 - -0.6 78,519 - -0.2 78,519 - -1.8 78,520 + +0.2 78,521 - +0.4 78,531 + +0.2 78,531 + -1.0 78,531 + -1.3 78,531 + +0.7 78,532 - -0.7 78,532 - +2.0 78,702 + GFF4716 0.18 +0.0 78,795 + GFF4716 0.29 +1.5 78,795 + GFF4716 0.29 +1.1 78,795 + GFF4716 0.29 -0.5 78,795 + GFF4716 0.29 -0.9 78,796 - GFF4716 0.30 -0.7 78,796 - GFF4716 0.30 -0.1 78,927 + GFF4716 0.45 -0.8 78,927 + GFF4716 0.45 -0.4 78,927 + GFF4716 0.45 +0.7 78,927 + GFF4716 0.45 -0.5 78,927 + GFF4716 0.45 -0.6 78,927 + GFF4716 0.45 -0.5 78,927 + GFF4716 0.45 -0.9 78,927 + GFF4716 0.45 -1.9 78,927 + GFF4716 0.45 -0.4 78,927 + GFF4716 0.45 -1.1 78,928 - GFF4716 0.45 -0.2 78,928 - GFF4716 0.45 +0.5 78,928 - GFF4716 0.45 +0.1 78,928 - GFF4716 0.45 -1.2 78,928 - GFF4716 0.45 -0.3 78,928 - GFF4716 0.45 -0.5 78,928 - GFF4716 0.45 -0.4 78,928 - GFF4716 0.45 +0.1 79,185 + GFF4716 0.76 -0.2 79,185 + GFF4716 0.76 -0.4 79,185 + GFF4716 0.76 -0.3 79,185 + GFF4716 0.76 +0.0 79,185 + GFF4716 0.76 -0.2 79,185 + GFF4716 0.76 -1.2 79,185 + GFF4716 0.76 -0.1 79,185 + GFF4716 0.76 -1.6 79,185 + GFF4716 0.76 +0.9 79,185 + GFF4716 0.76 +0.3 79,185 + GFF4716 0.76 +0.4 79,185 + GFF4716 0.76 +0.1 79,185 + GFF4716 0.76 +0.5 79,185 + GFF4716 0.76 -0.7 79,185 + GFF4716 0.76 -0.6 79,185 + GFF4716 0.76 -0.9 79,186 - GFF4716 0.76 -1.8 79,186 - GFF4716 0.76 -0.6 79,186 - GFF4716 0.76 -1.9 79,186 - GFF4716 0.76 -0.2 79,186 - GFF4716 0.76 -1.5 79,186 - GFF4716 0.76 -1.0 79,186 - GFF4716 0.76 -0.8 79,186 - GFF4716 0.76 -0.6 79,186 - GFF4716 0.76 +0.6 79,186 - GFF4716 0.76 +0.5 79,186 - GFF4716 0.76 +1.5 79,186 - GFF4716 0.76 -0.2 79,186 - GFF4716 0.76 -2.9 79,186 - GFF4716 0.76 -0.2 79,186 - GFF4716 0.76 -0.7 79,186 - GFF4716 0.76 +0.5 79,186 - GFF4716 0.76 +1.9 79,186 - GFF4716 0.76 -0.7 79,203 + GFF4716 0.78 +0.3 79,525 + GFF4717 0.17 -0.4 79,526 - GFF4717 0.17 -0.5 79,585 + GFF4717 0.24 -0.0 79,585 + GFF4717 0.24 -1.9 79,586 - GFF4717 0.24 -0.2 79,696 + GFF4717 0.38 +0.4 79,696 + GFF4717 0.38 -0.1 79,696 + GFF4717 0.38 -1.3 79,696 + GFF4717 0.38 -1.0 79,696 + GFF4717 0.38 -0.2 79,696 + GFF4717 0.38 -0.1 79,696 + GFF4717 0.38 -0.3 79,697 - GFF4717 0.38 -0.2 79,697 - GFF4717 0.38 -1.1 79,697 - GFF4717 0.38 +0.4 79,747 + GFF4717 0.44 -0.3 79,747 + GFF4717 0.44 -0.1 79,747 + GFF4717 0.44 -0.9 79,747 + GFF4717 0.44 -0.2 79,747 + GFF4717 0.44 +0.6 79,747 + GFF4717 0.44 +0.4 79,748 - GFF4717 0.44 -0.7 79,748 - GFF4717 0.44 -1.1 79,748 - GFF4717 0.44 +0.5 79,748 - GFF4717 0.44 -0.3 79,750 + GFF4717 0.45 -0.6 79,819 + GFF4717 0.53 +0.4 79,834 + GFF4717 0.55 -0.1 80,044 + GFF4717 0.82 -0.5 80,045 - GFF4717 0.82 -0.5 80,045 - GFF4717 0.82 +2.1 80,074 + GFF4717 0.85 +1.1 80,074 + GFF4717 0.85 +1.1 80,075 - GFF4717 0.85 +0.5 80,075 - GFF4717 0.85 -0.4 80,075 - GFF4717 0.85 -0.8 80,075 - GFF4717 0.85 -1.4 80,075 - GFF4717 0.85 -0.8 80,093 - GFF4717 0.88 -0.6 80,093 - GFF4717 0.88 -0.9 80,093 - GFF4717 0.88 -0.2 80,113 + +0.9 80,114 - +0.6 80,114 - +0.1 80,114 - -0.5 80,114 - +0.0 80,114 - +0.7 80,179 + +0.9
Or see this region's nucleotide sequence