Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4693 and GFF4694 are separated by 69 nucleotides GFF4694 and GFF4695 are separated by 14 nucleotides GFF4695 and GFF4696 are separated by 121 nucleotides GFF4696 and GFF4697 are separated by 104 nucleotides
GFF4693 - hypothetical protein, at 57,432 to 58,223
GFF4693
GFF4694 - no description, at 58,293 to 58,664
GFF4694
GFF4695 - Molybdopterin adenylyltransferase (EC 2.7.7.75), at 58,679 to 59,269
GFF4695
GFF4696 - UPF0307 protein YjgA, at 59,391 to 60,047
GFF4696
GFF4697 - TldE protein, part of TldE/TldD proteolytic complex, at 60,152 to 61,522
GFF4697
Position (kb)
58
59
60 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 57.879 kb on + strand, within GFF4693 at 57.879 kb on + strand, within GFF4693 at 57.879 kb on + strand, within GFF4693 at 57.879 kb on + strand, within GFF4693 at 57.879 kb on + strand, within GFF4693 at 57.879 kb on + strand, within GFF4693 at 57.879 kb on + strand, within GFF4693 at 57.879 kb on + strand, within GFF4693 at 57.879 kb on + strand, within GFF4693 at 57.879 kb on + strand, within GFF4693 at 57.879 kb on + strand, within GFF4693 at 57.880 kb on - strand, within GFF4693 at 57.880 kb on - strand, within GFF4693 at 57.880 kb on - strand, within GFF4693 at 57.880 kb on - strand, within GFF4693 at 57.880 kb on - strand, within GFF4693 at 57.880 kb on - strand, within GFF4693 at 57.880 kb on - strand, within GFF4693 at 57.880 kb on - strand, within GFF4693 at 57.880 kb on - strand, within GFF4693 at 57.880 kb on - strand, within GFF4693 at 57.880 kb on - strand, within GFF4693 at 58.155 kb on + strand at 58.155 kb on + strand at 58.156 kb on - strand at 58.156 kb on - strand at 58.221 kb on + strand at 58.221 kb on + strand at 58.221 kb on + strand at 58.222 kb on - strand at 58.298 kb on + strand at 58.298 kb on + strand at 58.298 kb on + strand at 58.298 kb on + strand at 58.298 kb on + strand at 58.298 kb on + strand at 58.299 kb on - strand at 58.299 kb on - strand at 58.299 kb on - strand at 58.302 kb on + strand at 58.302 kb on + strand at 58.302 kb on + strand at 58.303 kb on - strand at 58.303 kb on - strand at 58.303 kb on - strand at 58.303 kb on - strand at 58.303 kb on - strand at 58.303 kb on - strand at 58.303 kb on - strand at 58.521 kb on + strand, within GFF4694 at 58.521 kb on + strand, within GFF4694 at 58.521 kb on + strand, within GFF4694 at 58.521 kb on + strand, within GFF4694 at 58.522 kb on - strand, within GFF4694 at 58.522 kb on - strand, within GFF4694 at 58.522 kb on - strand, within GFF4694 at 58.552 kb on + strand, within GFF4694 at 58.553 kb on - strand, within GFF4694 at 58.553 kb on - strand, within GFF4694 at 58.553 kb on - strand, within GFF4694 at 58.591 kb on - strand, within GFF4694 at 58.662 kb on + strand at 58.746 kb on + strand, within GFF4695 at 58.747 kb on - strand, within GFF4695 at 58.747 kb on - strand, within GFF4695 at 58.773 kb on + strand, within GFF4695 at 59.002 kb on + strand, within GFF4695 at 59.002 kb on + strand, within GFF4695 at 59.002 kb on + strand, within GFF4695 at 59.002 kb on + strand, within GFF4695 at 59.002 kb on + strand, within GFF4695 at 59.003 kb on - strand, within GFF4695 at 59.202 kb on + strand, within GFF4695 at 59.202 kb on + strand, within GFF4695 at 59.250 kb on - strand at 60.022 kb on + strand at 60.114 kb on - strand at 60.137 kb on + strand at 60.137 kb on + strand at 60.137 kb on + strand at 60.137 kb on + strand at 60.137 kb on + strand at 60.138 kb on - strand at 60.138 kb on - strand at 60.138 kb on - strand at 60.138 kb on - strand at 60.138 kb on - strand at 60.138 kb on - strand at 60.138 kb on - strand at 60.138 kb on - strand at 60.138 kb on - strand at 60.138 kb on - strand at 60.138 kb on - strand at 60.138 kb on - strand at 60.142 kb on + strand at 60.142 kb on + strand at 60.142 kb on + strand at 60.142 kb on + strand at 60.143 kb on - strand at 60.191 kb on + strand at 60.191 kb on + strand at 60.191 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 57,879 + GFF4693 0.56 +0.2 57,879 + GFF4693 0.56 -0.5 57,879 + GFF4693 0.56 +0.1 57,879 + GFF4693 0.56 +0.1 57,879 + GFF4693 0.56 -1.0 57,879 + GFF4693 0.56 -0.0 57,879 + GFF4693 0.56 +0.1 57,879 + GFF4693 0.56 -0.9 57,879 + GFF4693 0.56 -0.7 57,879 + GFF4693 0.56 +0.7 57,879 + GFF4693 0.56 -0.1 57,880 - GFF4693 0.57 +1.1 57,880 - GFF4693 0.57 -0.2 57,880 - GFF4693 0.57 -0.0 57,880 - GFF4693 0.57 -0.7 57,880 - GFF4693 0.57 +0.7 57,880 - GFF4693 0.57 +0.2 57,880 - GFF4693 0.57 -0.7 57,880 - GFF4693 0.57 +0.2 57,880 - GFF4693 0.57 -0.0 57,880 - GFF4693 0.57 +1.5 57,880 - GFF4693 0.57 +2.5 58,155 + -0.1 58,155 + -0.3 58,156 - +1.0 58,156 - +0.2 58,221 + -0.6 58,221 + -0.1 58,221 + +0.3 58,222 - -0.0 58,298 + -0.3 58,298 + -0.9 58,298 + -0.5 58,298 + -0.9 58,298 + -0.1 58,298 + +1.1 58,299 - -2.3 58,299 - -0.5 58,299 - +0.2 58,302 + +0.0 58,302 + -0.8 58,302 + -0.7 58,303 - +0.5 58,303 - +0.0 58,303 - -0.1 58,303 - +0.2 58,303 - -1.1 58,303 - -1.9 58,303 - +0.1 58,521 + GFF4694 0.61 -0.8 58,521 + GFF4694 0.61 -1.1 58,521 + GFF4694 0.61 +1.1 58,521 + GFF4694 0.61 +0.7 58,522 - GFF4694 0.62 -0.8 58,522 - GFF4694 0.62 -0.6 58,522 - GFF4694 0.62 -0.1 58,552 + GFF4694 0.70 -0.7 58,553 - GFF4694 0.70 +0.1 58,553 - GFF4694 0.70 +0.7 58,553 - GFF4694 0.70 +1.5 58,591 - GFF4694 0.80 -1.3 58,662 + -0.4 58,746 + GFF4695 0.11 +0.3 58,747 - GFF4695 0.12 -0.3 58,747 - GFF4695 0.12 -0.1 58,773 + GFF4695 0.16 +0.1 59,002 + GFF4695 0.55 +0.7 59,002 + GFF4695 0.55 +1.5 59,002 + GFF4695 0.55 +0.7 59,002 + GFF4695 0.55 +0.5 59,002 + GFF4695 0.55 +0.1 59,003 - GFF4695 0.55 +0.3 59,202 + GFF4695 0.88 -0.5 59,202 + GFF4695 0.88 +1.5 59,250 - +0.0 60,022 + -0.1 60,114 - -0.0 60,137 + -0.3 60,137 + -0.1 60,137 + -0.5 60,137 + -0.5 60,137 + -0.1 60,138 - -0.5 60,138 - +0.7 60,138 - -1.1 60,138 - +0.0 60,138 - -1.5 60,138 - -0.3 60,138 - +1.1 60,138 - +0.7 60,138 - +0.1 60,138 - +0.2 60,138 - +3.1 60,138 - -0.8 60,142 + -0.1 60,142 + +0.6 60,142 + -0.3 60,142 + +0.6 60,143 - +0.3 60,191 + +0.2 60,191 + -0.2 60,191 + +0.5
Or see this region's nucleotide sequence