Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4496 and GFF4497 overlap by 8 nucleotides GFF4497 and GFF4498 are separated by 8 nucleotides GFF4498 and GFF4499 overlap by 4 nucleotides GFF4499 and GFF4500 are separated by 17 nucleotides
GFF4496 - Uncharacterized MFS-type transporter, at 139,940 to 141,517
GFF4496
GFF4497 - FIG027115: Membrane protein, at 141,510 to 141,908
GFF4497
GFF4498 - Exoenzymes regulatory protein AepA precursor, at 141,917 to 143,911
GFF4498
GFF4499 - Uncharacterized DUF1427 protein PA2697, at 143,908 to 144,201
GFF4499
GFF4500 - FIG002283: Isochorismatase family protein, at 144,219 to 144,872
GFF4500
Position (kb)
141
142
143
144 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 140.964 kb on + strand, within GFF4496 at 140.964 kb on + strand, within GFF4496 at 140.964 kb on + strand, within GFF4496 at 140.964 kb on + strand, within GFF4496 at 140.964 kb on + strand, within GFF4496 at 140.964 kb on + strand, within GFF4496 at 140.964 kb on + strand, within GFF4496 at 140.964 kb on + strand, within GFF4496 at 140.965 kb on - strand, within GFF4496 at 140.965 kb on - strand, within GFF4496 at 140.965 kb on - strand, within GFF4496 at 140.965 kb on - strand, within GFF4496 at 140.965 kb on - strand, within GFF4496 at 140.965 kb on - strand, within GFF4496 at 140.965 kb on - strand, within GFF4496 at 140.965 kb on - strand, within GFF4496 at 140.965 kb on - strand, within GFF4496 at 140.965 kb on - strand, within GFF4496 at 140.965 kb on - strand, within GFF4496 at 140.965 kb on - strand, within GFF4496 at 140.965 kb on - strand, within GFF4496 at 140.985 kb on + strand, within GFF4496 at 140.985 kb on + strand, within GFF4496 at 140.985 kb on + strand, within GFF4496 at 140.985 kb on + strand, within GFF4496 at 140.985 kb on + strand, within GFF4496 at 140.986 kb on - strand, within GFF4496 at 140.986 kb on - strand, within GFF4496 at 141.186 kb on + strand, within GFF4496 at 141.187 kb on + strand, within GFF4496 at 141.187 kb on - strand, within GFF4496 at 141.187 kb on - strand, within GFF4496 at 141.187 kb on - strand, within GFF4496 at 141.187 kb on - strand, within GFF4496 at 141.187 kb on - strand, within GFF4496 at 141.589 kb on + strand, within GFF4497 at 141.847 kb on + strand, within GFF4497 at 141.848 kb on - strand, within GFF4497 at 141.848 kb on - strand, within GFF4497 at 141.848 kb on - strand, within GFF4497 at 142.110 kb on + strand at 142.111 kb on - strand at 142.197 kb on + strand, within GFF4498 at 142.197 kb on + strand, within GFF4498 at 142.197 kb on + strand, within GFF4498 at 142.197 kb on + strand, within GFF4498 at 142.198 kb on - strand, within GFF4498 at 142.473 kb on + strand, within GFF4498 at 142.473 kb on + strand, within GFF4498 at 142.474 kb on - strand, within GFF4498 at 142.474 kb on - strand, within GFF4498 at 142.474 kb on - strand, within GFF4498 at 142.474 kb on - strand, within GFF4498 at 142.474 kb on - strand, within GFF4498 at 142.474 kb on - strand, within GFF4498 at 142.474 kb on - strand, within GFF4498 at 142.573 kb on - strand, within GFF4498 at 142.588 kb on - strand, within GFF4498 at 142.588 kb on - strand, within GFF4498 at 142.588 kb on - strand, within GFF4498 at 142.599 kb on + strand, within GFF4498 at 142.599 kb on + strand, within GFF4498 at 142.667 kb on - strand, within GFF4498 at 142.715 kb on - strand, within GFF4498 at 142.715 kb on - strand, within GFF4498 at 142.715 kb on - strand, within GFF4498 at 142.715 kb on - strand, within GFF4498 at 142.715 kb on - strand, within GFF4498 at 142.767 kb on + strand, within GFF4498 at 142.767 kb on + strand, within GFF4498 at 142.767 kb on + strand, within GFF4498 at 142.767 kb on + strand, within GFF4498 at 142.767 kb on + strand, within GFF4498 at 142.768 kb on - strand, within GFF4498 at 142.768 kb on - strand, within GFF4498 at 142.768 kb on - strand, within GFF4498 at 142.933 kb on - strand, within GFF4498 at 142.933 kb on - strand, within GFF4498 at 142.933 kb on - strand, within GFF4498 at 142.950 kb on + strand, within GFF4498 at 142.950 kb on + strand, within GFF4498 at 142.950 kb on + strand, within GFF4498 at 142.951 kb on - strand, within GFF4498 at 142.951 kb on - strand, within GFF4498 at 142.951 kb on - strand, within GFF4498 at 142.951 kb on - strand, within GFF4498 at 142.951 kb on - strand, within GFF4498 at 142.951 kb on - strand, within GFF4498 at 142.951 kb on - strand, within GFF4498 at 143.019 kb on + strand, within GFF4498 at 143.019 kb on + strand, within GFF4498 at 143.019 kb on + strand, within GFF4498 at 143.019 kb on + strand, within GFF4498 at 143.019 kb on + strand, within GFF4498 at 143.019 kb on + strand, within GFF4498 at 143.019 kb on + strand, within GFF4498 at 143.019 kb on + strand, within GFF4498 at 143.019 kb on + strand, within GFF4498 at 143.020 kb on - strand, within GFF4498 at 143.020 kb on - strand, within GFF4498 at 143.020 kb on - strand, within GFF4498 at 143.020 kb on - strand, within GFF4498 at 143.020 kb on - strand, within GFF4498 at 143.020 kb on - strand, within GFF4498 at 143.020 kb on - strand, within GFF4498 at 143.020 kb on - strand, within GFF4498 at 143.020 kb on - strand, within GFF4498 at 143.073 kb on + strand, within GFF4498 at 143.073 kb on + strand, within GFF4498 at 143.074 kb on - strand, within GFF4498 at 143.074 kb on - strand, within GFF4498 at 143.175 kb on + strand, within GFF4498 at 143.175 kb on + strand, within GFF4498 at 143.215 kb on - strand, within GFF4498 at 143.215 kb on - strand, within GFF4498 at 143.316 kb on + strand, within GFF4498 at 143.316 kb on + strand, within GFF4498 at 143.317 kb on - strand, within GFF4498 at 143.317 kb on - strand, within GFF4498 at 143.361 kb on + strand, within GFF4498 at 143.361 kb on + strand, within GFF4498 at 143.361 kb on + strand, within GFF4498 at 143.362 kb on - strand, within GFF4498 at 143.362 kb on - strand, within GFF4498 at 143.362 kb on - strand, within GFF4498 at 143.362 kb on - strand, within GFF4498 at 144.149 kb on + strand, within GFF4499 at 144.149 kb on + strand, within GFF4499 at 144.149 kb on + strand, within GFF4499 at 144.149 kb on + strand, within GFF4499 at 144.149 kb on + strand, within GFF4499 at 144.149 kb on + strand, within GFF4499 at 144.149 kb on + strand, within GFF4499 at 144.150 kb on - strand, within GFF4499 at 144.150 kb on - strand, within GFF4499 at 144.150 kb on - strand, within GFF4499 at 144.150 kb on - strand, within GFF4499 at 144.150 kb on - strand, within GFF4499 at 144.150 kb on - strand, within GFF4499 at 144.191 kb on + strand at 144.191 kb on + strand at 144.191 kb on + strand at 144.191 kb on + strand at 144.191 kb on + strand at 144.191 kb on + strand at 144.191 kb on + strand at 144.191 kb on + strand at 144.191 kb on + strand at 144.192 kb on - strand at 144.192 kb on - strand at 144.192 kb on - strand at 144.192 kb on - strand at 144.192 kb on - strand at 144.192 kb on - strand at 144.192 kb on - strand at 144.192 kb on - strand at 144.192 kb on - strand at 144.192 kb on - strand at 144.253 kb on + strand at 144.253 kb on + strand at 144.253 kb on + strand at 144.253 kb on + strand at 144.253 kb on + strand at 144.254 kb on - strand at 144.254 kb on - strand at 144.254 kb on - strand at 144.254 kb on - strand at 144.271 kb on + strand at 144.272 kb on - strand at 144.313 kb on + strand, within GFF4500 at 144.313 kb on + strand, within GFF4500 at 144.314 kb on - strand, within GFF4500 at 144.314 kb on - strand, within GFF4500 at 144.314 kb on - strand, within GFF4500 at 144.314 kb on - strand, within GFF4500 at 144.355 kb on + strand, within GFF4500 at 144.355 kb on + strand, within GFF4500 at 144.355 kb on + strand, within GFF4500 at 144.355 kb on + strand, within GFF4500 at 144.356 kb on - strand, within GFF4500 at 144.356 kb on - strand, within GFF4500 at 144.356 kb on - strand, within GFF4500 at 144.457 kb on + strand, within GFF4500 at 144.457 kb on + strand, within GFF4500 at 144.457 kb on + strand, within GFF4500 at 144.457 kb on + strand, within GFF4500 at 144.457 kb on + strand, within GFF4500 at 144.457 kb on + strand, within GFF4500 at 144.458 kb on - strand, within GFF4500 at 144.458 kb on - strand, within GFF4500 at 144.458 kb on - strand, within GFF4500 at 144.458 kb on - strand, within GFF4500 at 144.458 kb on - strand, within GFF4500 at 144.458 kb on - strand, within GFF4500 at 144.458 kb on - strand, within GFF4500 at 144.458 kb on - strand, within GFF4500 at 144.458 kb on - strand, within GFF4500 at 144.458 kb on - strand, within GFF4500 at 144.458 kb on - strand, within GFF4500 at 144.458 kb on - strand, within GFF4500 at 144.458 kb on - strand, within GFF4500 at 144.458 kb on - strand, within GFF4500 at 144.458 kb on - strand, within GFF4500
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 140,964 + GFF4496 0.65 -0.1 140,964 + GFF4496 0.65 -0.3 140,964 + GFF4496 0.65 +1.0 140,964 + GFF4496 0.65 +1.8 140,964 + GFF4496 0.65 -0.5 140,964 + GFF4496 0.65 -0.4 140,964 + GFF4496 0.65 -0.4 140,964 + GFF4496 0.65 -0.9 140,965 - GFF4496 0.65 -0.8 140,965 - GFF4496 0.65 -0.2 140,965 - GFF4496 0.65 -1.2 140,965 - GFF4496 0.65 -0.3 140,965 - GFF4496 0.65 +0.9 140,965 - GFF4496 0.65 -1.1 140,965 - GFF4496 0.65 +0.1 140,965 - GFF4496 0.65 +0.2 140,965 - GFF4496 0.65 +0.1 140,965 - GFF4496 0.65 +1.4 140,965 - GFF4496 0.65 -0.7 140,965 - GFF4496 0.65 -0.0 140,965 - GFF4496 0.65 -1.2 140,985 + GFF4496 0.66 -1.2 140,985 + GFF4496 0.66 -0.4 140,985 + GFF4496 0.66 -1.0 140,985 + GFF4496 0.66 -1.2 140,985 + GFF4496 0.66 +1.0 140,986 - GFF4496 0.66 +0.5 140,986 - GFF4496 0.66 -0.8 141,186 + GFF4496 0.79 +1.2 141,187 + GFF4496 0.79 -0.4 141,187 - GFF4496 0.79 +0.6 141,187 - GFF4496 0.79 -0.1 141,187 - GFF4496 0.79 +0.9 141,187 - GFF4496 0.79 -1.4 141,187 - GFF4496 0.79 -0.4 141,589 + GFF4497 0.20 -0.4 141,847 + GFF4497 0.84 -0.1 141,848 - GFF4497 0.85 -0.5 141,848 - GFF4497 0.85 -1.4 141,848 - GFF4497 0.85 -1.8 142,110 + +0.3 142,111 - -0.4 142,197 + GFF4498 0.14 +0.6 142,197 + GFF4498 0.14 -1.2 142,197 + GFF4498 0.14 -0.6 142,197 + GFF4498 0.14 -0.6 142,198 - GFF4498 0.14 -0.0 142,473 + GFF4498 0.28 +0.5 142,473 + GFF4498 0.28 -1.5 142,474 - GFF4498 0.28 -0.9 142,474 - GFF4498 0.28 -0.4 142,474 - GFF4498 0.28 -0.3 142,474 - GFF4498 0.28 +0.5 142,474 - GFF4498 0.28 +1.6 142,474 - GFF4498 0.28 -0.4 142,474 - GFF4498 0.28 +2.2 142,573 - GFF4498 0.33 +0.8 142,588 - GFF4498 0.34 -0.0 142,588 - GFF4498 0.34 +0.5 142,588 - GFF4498 0.34 -0.2 142,599 + GFF4498 0.34 +0.2 142,599 + GFF4498 0.34 -0.3 142,667 - GFF4498 0.38 +0.3 142,715 - GFF4498 0.40 +0.6 142,715 - GFF4498 0.40 +0.2 142,715 - GFF4498 0.40 +1.6 142,715 - GFF4498 0.40 -0.4 142,715 - GFF4498 0.40 +0.5 142,767 + GFF4498 0.43 -0.9 142,767 + GFF4498 0.43 +0.8 142,767 + GFF4498 0.43 +0.5 142,767 + GFF4498 0.43 -0.4 142,767 + GFF4498 0.43 +0.3 142,768 - GFF4498 0.43 +0.4 142,768 - GFF4498 0.43 -0.1 142,768 - GFF4498 0.43 +0.7 142,933 - GFF4498 0.51 -0.1 142,933 - GFF4498 0.51 +1.2 142,933 - GFF4498 0.51 +0.5 142,950 + GFF4498 0.52 +1.0 142,950 + GFF4498 0.52 -0.0 142,950 + GFF4498 0.52 -0.5 142,951 - GFF4498 0.52 -1.0 142,951 - GFF4498 0.52 -1.4 142,951 - GFF4498 0.52 -0.3 142,951 - GFF4498 0.52 -0.5 142,951 - GFF4498 0.52 -1.0 142,951 - GFF4498 0.52 -0.7 142,951 - GFF4498 0.52 -0.4 143,019 + GFF4498 0.55 -1.8 143,019 + GFF4498 0.55 -2.4 143,019 + GFF4498 0.55 +0.6 143,019 + GFF4498 0.55 -0.9 143,019 + GFF4498 0.55 +0.1 143,019 + GFF4498 0.55 +0.2 143,019 + GFF4498 0.55 -1.2 143,019 + GFF4498 0.55 -0.1 143,019 + GFF4498 0.55 +0.3 143,020 - GFF4498 0.55 +1.7 143,020 - GFF4498 0.55 +0.0 143,020 - GFF4498 0.55 -0.1 143,020 - GFF4498 0.55 -0.7 143,020 - GFF4498 0.55 -0.9 143,020 - GFF4498 0.55 -0.2 143,020 - GFF4498 0.55 +1.1 143,020 - GFF4498 0.55 +0.4 143,020 - GFF4498 0.55 -0.7 143,073 + GFF4498 0.58 +0.4 143,073 + GFF4498 0.58 -0.5 143,074 - GFF4498 0.58 -0.3 143,074 - GFF4498 0.58 -1.1 143,175 + GFF4498 0.63 -0.3 143,175 + GFF4498 0.63 +0.4 143,215 - GFF4498 0.65 -2.0 143,215 - GFF4498 0.65 +0.7 143,316 + GFF4498 0.70 +0.3 143,316 + GFF4498 0.70 -1.0 143,317 - GFF4498 0.70 +0.5 143,317 - GFF4498 0.70 -1.5 143,361 + GFF4498 0.72 +0.3 143,361 + GFF4498 0.72 +0.6 143,361 + GFF4498 0.72 +0.4 143,362 - GFF4498 0.72 -0.5 143,362 - GFF4498 0.72 -0.6 143,362 - GFF4498 0.72 -0.5 143,362 - GFF4498 0.72 -0.1 144,149 + GFF4499 0.82 +0.3 144,149 + GFF4499 0.82 -0.8 144,149 + GFF4499 0.82 -0.8 144,149 + GFF4499 0.82 -0.4 144,149 + GFF4499 0.82 -0.0 144,149 + GFF4499 0.82 -0.5 144,149 + GFF4499 0.82 -0.1 144,150 - GFF4499 0.82 -0.5 144,150 - GFF4499 0.82 +0.2 144,150 - GFF4499 0.82 -1.7 144,150 - GFF4499 0.82 +0.3 144,150 - GFF4499 0.82 -1.2 144,150 - GFF4499 0.82 +0.8 144,191 + -1.0 144,191 + -0.1 144,191 + -0.2 144,191 + -0.3 144,191 + +0.3 144,191 + -0.9 144,191 + -0.4 144,191 + +0.8 144,191 + -1.4 144,192 - -0.6 144,192 - -0.0 144,192 - -0.5 144,192 - -0.3 144,192 - +0.4 144,192 - -1.2 144,192 - +0.9 144,192 - -0.9 144,192 - +0.8 144,192 - +0.2 144,253 + +0.8 144,253 + -3.1 144,253 + -0.8 144,253 + -0.6 144,253 + +0.3 144,254 - +1.8 144,254 - +0.1 144,254 - +0.3 144,254 - +0.1 144,271 + +0.3 144,272 - -2.5 144,313 + GFF4500 0.14 -1.5 144,313 + GFF4500 0.14 +1.4 144,314 - GFF4500 0.15 -0.7 144,314 - GFF4500 0.15 +0.4 144,314 - GFF4500 0.15 +0.3 144,314 - GFF4500 0.15 -0.2 144,355 + GFF4500 0.21 -0.8 144,355 + GFF4500 0.21 +0.4 144,355 + GFF4500 0.21 +1.1 144,355 + GFF4500 0.21 -0.5 144,356 - GFF4500 0.21 -0.6 144,356 - GFF4500 0.21 -0.2 144,356 - GFF4500 0.21 -0.7 144,457 + GFF4500 0.36 +0.6 144,457 + GFF4500 0.36 +1.8 144,457 + GFF4500 0.36 -0.9 144,457 + GFF4500 0.36 -0.4 144,457 + GFF4500 0.36 -0.8 144,457 + GFF4500 0.36 -0.3 144,458 - GFF4500 0.37 -0.9 144,458 - GFF4500 0.37 -0.5 144,458 - GFF4500 0.37 -0.2 144,458 - GFF4500 0.37 -0.4 144,458 - GFF4500 0.37 +0.1 144,458 - GFF4500 0.37 +0.2 144,458 - GFF4500 0.37 -0.5 144,458 - GFF4500 0.37 -0.2 144,458 - GFF4500 0.37 -0.3 144,458 - GFF4500 0.37 +0.1 144,458 - GFF4500 0.37 -0.7 144,458 - GFF4500 0.37 -0.5 144,458 - GFF4500 0.37 +0.7 144,458 - GFF4500 0.37 -1.0 144,458 - GFF4500 0.37 +1.6
Or see this region's nucleotide sequence