Strain Fitness in Variovorax sp. SCN45 around GFF449

Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF447 and GFF448 overlap by 4 nucleotidesGFF448 and GFF449 overlap by 17 nucleotidesGFF449 and GFF450 overlap by 16 nucleotides GFF447 - Vitamin B12 ABC transporter, substrate-binding protein BtuF, at 485,147 to 486,019 GFF447 GFF448 - Adenosylcobinamide kinase (EC 2.7.1.156) / Adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62), at 486,016 to 486,621 GFF448 GFF449 - Cobyrinic acid a,c-diamide synthetase (EC 6.3.5.11), at 486,605 to 488,026 GFF449 GFF450 - Outer membrane vitamin B12 receptor BtuB, at 488,011 to 489,927 GFF450 Position (kb) 486 487 488 489Strain fitness (log2 ratio) -2 -1 0 1 2at 486.464 kb on + strand, within GFF448at 486.464 kb on + strand, within GFF448at 486.464 kb on + strand, within GFF448at 486.465 kb on - strand, within GFF448at 486.465 kb on - strand, within GFF448at 486.465 kb on - strand, within GFF448at 486.465 kb on - strand, within GFF448at 486.465 kb on - strand, within GFF448at 486.606 kb on + strandat 486.606 kb on + strandat 486.607 kb on - strandat 486.607 kb on - strandat 486.607 kb on - strandat 486.607 kb on - strandat 486.607 kb on - strandat 486.695 kb on + strandat 486.699 kb on + strandat 486.699 kb on + strandat 486.699 kb on + strandat 486.700 kb on - strandat 486.700 kb on - strandat 486.726 kb on + strandat 486.726 kb on + strandat 486.726 kb on + strandat 486.726 kb on + strandat 486.726 kb on + strandat 486.726 kb on + strandat 486.726 kb on + strandat 486.727 kb on - strandat 486.727 kb on - strandat 486.727 kb on - strandat 486.727 kb on - strandat 486.727 kb on - strandat 486.727 kb on - strandat 486.819 kb on + strand, within GFF449at 486.819 kb on + strand, within GFF449at 486.820 kb on - strand, within GFF449at 486.820 kb on - strand, within GFF449at 486.820 kb on - strand, within GFF449at 486.820 kb on - strand, within GFF449at 487.210 kb on - strand, within GFF449at 487.585 kb on - strand, within GFF449at 488.012 kb on + strandat 488.013 kb on - strandat 488.013 kb on - strandat 488.013 kb on - strandat 488.013 kb on - strandat 488.013 kb on - strandat 488.039 kb on + strandat 488.039 kb on + strandat 488.039 kb on + strandat 488.039 kb on + strandat 488.040 kb on - strandat 488.040 kb on - strandat 488.063 kb on + strandat 488.064 kb on - strandat 488.075 kb on + strandat 488.075 kb on + strandat 488.075 kb on + strandat 488.076 kb on - strandat 488.076 kb on - strandat 488.081 kb on + strandat 488.081 kb on + strandat 488.081 kb on + strandat 488.082 kb on - strandat 488.082 kb on - strandat 488.169 kb on - strandat 488.204 kb on + strand, within GFF450at 488.205 kb on - strand, within GFF450at 488.402 kb on + strand, within GFF450at 488.426 kb on + strand, within GFF450at 488.498 kb on + strand, within GFF450at 488.498 kb on + strand, within GFF450at 488.498 kb on + strand, within GFF450at 488.498 kb on + strand, within GFF450at 488.498 kb on + strand, within GFF450at 488.499 kb on - strand, within GFF450at 488.499 kb on - strand, within GFF450at 488.499 kb on - strand, within GFF450at 488.534 kb on + strand, within GFF450at 488.535 kb on - strand, within GFF450at 488.535 kb on - strand, within GFF450at 488.535 kb on - strand, within GFF450at 488.535 kb on - strand, within GFF450at 488.535 kb on - strand, within GFF450at 488.535 kb on - strand, within GFF450at 488.535 kb on - strand, within GFF450at 488.535 kb on - strand, within GFF450at 488.591 kb on + strand, within GFF450at 488.592 kb on - strand, within GFF450at 488.609 kb on + strand, within GFF450at 488.609 kb on + strand, within GFF450at 488.609 kb on + strand, within GFF450at 488.609 kb on + strand, within GFF450at 488.609 kb on + strand, within GFF450at 488.609 kb on + strand, within GFF450at 488.610 kb on - strand, within GFF450at 488.610 kb on - strand, within GFF450at 488.675 kb on + strand, within GFF450at 488.675 kb on + strand, within GFF450at 488.676 kb on - strand, within GFF450at 488.681 kb on + strand, within GFF450at 488.918 kb on + strand, within GFF450at 488.918 kb on + strand, within GFF450at 488.945 kb on + strand, within GFF450at 488.945 kb on + strand, within GFF450at 488.945 kb on + strand, within GFF450at 488.945 kb on + strand, within GFF450at 488.945 kb on + strand, within GFF450at 488.966 kb on + strand, within GFF450at 488.966 kb on + strand, within GFF450at 488.966 kb on + strand, within GFF450at 488.966 kb on + strand, within GFF450at 488.966 kb on + strand, within GFF450at 488.966 kb on + strand, within GFF450at 488.967 kb on - strand, within GFF450at 488.967 kb on - strand, within GFF450at 488.967 kb on - strand, within GFF450at 489.005 kb on + strand, within GFF450at 489.005 kb on + strand, within GFF450at 489.005 kb on + strand, within GFF450at 489.006 kb on - strand, within GFF450at 489.006 kb on - strand, within GFF450at 489.006 kb on - strand, within GFF450at 489.006 kb on - strand, within GFF450at 489.006 kb on - strand, within GFF450

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
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486,464 + GFF448 0.74 +0.1
486,464 + GFF448 0.74 -0.6
486,464 + GFF448 0.74 -0.6
486,465 - GFF448 0.74 +0.4
486,465 - GFF448 0.74 -1.8
486,465 - GFF448 0.74 +0.4
486,465 - GFF448 0.74 -0.3
486,465 - GFF448 0.74 +0.8
486,606 + +2.7
486,606 + -0.4
486,607 - -0.6
486,607 - +0.1
486,607 - -0.9
486,607 - -0.0
486,607 - +0.3
486,695 + +0.1
486,699 + -1.2
486,699 + -0.8
486,699 + -0.6
486,700 - +1.7
486,700 - +0.3
486,726 + -0.4
486,726 + -0.2
486,726 + -0.2
486,726 + -1.7
486,726 + -0.2
486,726 + +0.2
486,726 + -0.9
486,727 - -0.4
486,727 - +0.1
486,727 - +1.1
486,727 - +0.4
486,727 - -0.5
486,727 - -1.3
486,819 + GFF449 0.15 -0.8
486,819 + GFF449 0.15 -0.1
486,820 - GFF449 0.15 +0.6
486,820 - GFF449 0.15 -1.2
486,820 - GFF449 0.15 +1.4
486,820 - GFF449 0.15 +0.1
487,210 - GFF449 0.43 +1.4
487,585 - GFF449 0.69 -0.1
488,012 + +1.6
488,013 - -0.3
488,013 - -0.3
488,013 - +1.8
488,013 - -0.3
488,013 - -0.1
488,039 + -0.8
488,039 + +0.3
488,039 + -0.4
488,039 + +0.7
488,040 - -0.1
488,040 - -1.4
488,063 + +0.8
488,064 - -0.9
488,075 + +0.0
488,075 + -0.8
488,075 + +0.9
488,076 - -0.8
488,076 - -0.0
488,081 + -0.2
488,081 + -0.6
488,081 + -0.2
488,082 - -0.1
488,082 - +0.8
488,169 - +0.2
488,204 + GFF450 0.10 -0.2
488,205 - GFF450 0.10 -0.0
488,402 + GFF450 0.20 -0.3
488,426 + GFF450 0.22 -0.2
488,498 + GFF450 0.25 -1.0
488,498 + GFF450 0.25 +1.4
488,498 + GFF450 0.25 -0.5
488,498 + GFF450 0.25 -1.1
488,498 + GFF450 0.25 -0.8
488,499 - GFF450 0.25 +0.8
488,499 - GFF450 0.25 +0.3
488,499 - GFF450 0.25 +0.9
488,534 + GFF450 0.27 +0.1
488,535 - GFF450 0.27 -1.4
488,535 - GFF450 0.27 -0.5
488,535 - GFF450 0.27 -1.8
488,535 - GFF450 0.27 +0.9
488,535 - GFF450 0.27 -0.6
488,535 - GFF450 0.27 -0.1
488,535 - GFF450 0.27 -0.9
488,535 - GFF450 0.27 +0.1
488,591 + GFF450 0.30 -0.1
488,592 - GFF450 0.30 +0.2
488,609 + GFF450 0.31 +0.9
488,609 + GFF450 0.31 -2.2
488,609 + GFF450 0.31 +0.2
488,609 + GFF450 0.31 -0.3
488,609 + GFF450 0.31 +0.6
488,609 + GFF450 0.31 -1.2
488,610 - GFF450 0.31 -0.9
488,610 - GFF450 0.31 -0.2
488,675 + GFF450 0.35 -0.2
488,675 + GFF450 0.35 -0.5
488,676 - GFF450 0.35 +0.7
488,681 + GFF450 0.35 +0.2
488,918 + GFF450 0.47 -0.7
488,918 + GFF450 0.47 +0.1
488,945 + GFF450 0.49 -0.0
488,945 + GFF450 0.49 -1.3
488,945 + GFF450 0.49 +0.2
488,945 + GFF450 0.49 -0.8
488,945 + GFF450 0.49 +1.8
488,966 + GFF450 0.50 -0.8
488,966 + GFF450 0.50 -0.2
488,966 + GFF450 0.50 +0.4
488,966 + GFF450 0.50 +0.6
488,966 + GFF450 0.50 -1.4
488,966 + GFF450 0.50 -0.4
488,967 - GFF450 0.50 +1.1
488,967 - GFF450 0.50 +0.4
488,967 - GFF450 0.50 -0.1
489,005 + GFF450 0.52 +0.7
489,005 + GFF450 0.52 -1.0
489,005 + GFF450 0.52 -1.3
489,006 - GFF450 0.52 +0.8
489,006 - GFF450 0.52 -0.7
489,006 - GFF450 0.52 -1.3
489,006 - GFF450 0.52 +1.4
489,006 - GFF450 0.52 +0.1

Or see this region's nucleotide sequence