Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4272 and GFF4273 are separated by 8 nucleotides GFF4273 and GFF4274 are separated by 35 nucleotides GFF4274 and GFF4275 are separated by 60 nucleotides
GFF4272 - BUG/TctC family periplasmic protein, at 192,302 to 193,282
GFF4272
GFF4273 - BUG/TctC family periplasmic protein, at 193,291 to 194,259
GFF4273
GFF4274 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 194,295 to 195,011
GFF4274
GFF4275 - 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases, at 195,072 to 196,718
GFF4275
Position (kb) 193
194
195 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 192.410 kb on + strand, within GFF4272 at 192.533 kb on + strand, within GFF4272 at 192.533 kb on + strand, within GFF4272 at 192.533 kb on + strand, within GFF4272 at 192.533 kb on + strand, within GFF4272 at 192.534 kb on - strand, within GFF4272 at 192.534 kb on - strand, within GFF4272 at 192.534 kb on - strand, within GFF4272 at 192.534 kb on - strand, within GFF4272 at 192.612 kb on - strand, within GFF4272 at 192.612 kb on - strand, within GFF4272 at 192.765 kb on - strand, within GFF4272 at 192.765 kb on - strand, within GFF4272 at 192.765 kb on - strand, within GFF4272 at 192.765 kb on - strand, within GFF4272 at 192.765 kb on - strand, within GFF4272 at 192.765 kb on - strand, within GFF4272 at 193.386 kb on - strand at 193.386 kb on - strand at 193.782 kb on - strand, within GFF4273 at 193.782 kb on - strand, within GFF4273 at 193.782 kb on - strand, within GFF4273 at 193.805 kb on - strand, within GFF4273 at 193.997 kb on + strand, within GFF4273 at 193.998 kb on - strand, within GFF4273 at 194.276 kb on + strand at 194.276 kb on + strand at 194.276 kb on + strand at 194.277 kb on - strand at 194.277 kb on - strand at 194.277 kb on - strand at 194.277 kb on - strand at 194.278 kb on + strand at 194.445 kb on + strand, within GFF4274 at 194.605 kb on + strand, within GFF4274 at 194.605 kb on + strand, within GFF4274 at 194.606 kb on - strand, within GFF4274 at 194.606 kb on - strand, within GFF4274 at 194.798 kb on + strand, within GFF4274 at 194.852 kb on - strand, within GFF4274 at 194.879 kb on - strand, within GFF4274
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 192,410 + GFF4272 0.11 +0.8 192,533 + GFF4272 0.24 -0.5 192,533 + GFF4272 0.24 -1.5 192,533 + GFF4272 0.24 -0.1 192,533 + GFF4272 0.24 +0.5 192,534 - GFF4272 0.24 -0.7 192,534 - GFF4272 0.24 +1.1 192,534 - GFF4272 0.24 -0.3 192,534 - GFF4272 0.24 -0.6 192,612 - GFF4272 0.32 +0.1 192,612 - GFF4272 0.32 -2.5 192,765 - GFF4272 0.47 -0.5 192,765 - GFF4272 0.47 +0.0 192,765 - GFF4272 0.47 +0.5 192,765 - GFF4272 0.47 -0.2 192,765 - GFF4272 0.47 -0.8 192,765 - GFF4272 0.47 +0.9 193,386 - -0.3 193,386 - -0.6 193,782 - GFF4273 0.51 -1.4 193,782 - GFF4273 0.51 +0.5 193,782 - GFF4273 0.51 -0.9 193,805 - GFF4273 0.53 -0.8 193,997 + GFF4273 0.73 -0.9 193,998 - GFF4273 0.73 -1.6 194,276 + -0.4 194,276 + -0.1 194,276 + +1.4 194,277 - -2.8 194,277 - -0.3 194,277 - -1.9 194,277 - +0.8 194,278 + -0.9 194,445 + GFF4274 0.21 +0.5 194,605 + GFF4274 0.43 -0.4 194,605 + GFF4274 0.43 -0.5 194,606 - GFF4274 0.43 +0.2 194,606 - GFF4274 0.43 -1.1 194,798 + GFF4274 0.70 +0.8 194,852 - GFF4274 0.78 +0.3 194,879 - GFF4274 0.81 +0.8
Or see this region's nucleotide sequence