Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4249 and GFF4250 are separated by 59 nucleotides GFF4250 and GFF4251 are separated by 102 nucleotides GFF4251 and GFF4252 are separated by 81 nucleotides
GFF4249 - hypothetical protein, at 164,095 to 165,507
GFF4249
GFF4250 - Transcriptional regulator, AcrR family, at 165,567 to 166,118
GFF4250
GFF4251 - no description, at 166,221 to 166,895
GFF4251
GFF4252 - hypothetical protein, at 166,977 to 168,728
GFF4252
Position (kb)
165
166
167 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 164.668 kb on + strand, within GFF4249 at 164.668 kb on + strand, within GFF4249 at 164.668 kb on + strand, within GFF4249 at 164.668 kb on + strand, within GFF4249 at 164.669 kb on - strand, within GFF4249 at 164.669 kb on - strand, within GFF4249 at 164.669 kb on - strand, within GFF4249 at 164.813 kb on - strand, within GFF4249 at 164.851 kb on + strand, within GFF4249 at 164.851 kb on + strand, within GFF4249 at 164.851 kb on + strand, within GFF4249 at 164.851 kb on + strand, within GFF4249 at 164.852 kb on - strand, within GFF4249 at 164.852 kb on - strand, within GFF4249 at 164.852 kb on - strand, within GFF4249 at 164.852 kb on - strand, within GFF4249 at 164.954 kb on - strand, within GFF4249 at 164.976 kb on + strand, within GFF4249 at 165.003 kb on + strand, within GFF4249 at 165.003 kb on + strand, within GFF4249 at 165.003 kb on + strand, within GFF4249 at 165.004 kb on - strand, within GFF4249 at 165.004 kb on - strand, within GFF4249 at 165.091 kb on + strand, within GFF4249 at 165.091 kb on + strand, within GFF4249 at 165.092 kb on - strand, within GFF4249 at 165.092 kb on - strand, within GFF4249 at 165.092 kb on - strand, within GFF4249 at 165.127 kb on + strand, within GFF4249 at 165.128 kb on - strand, within GFF4249 at 165.235 kb on + strand, within GFF4249 at 165.235 kb on + strand, within GFF4249 at 165.235 kb on + strand, within GFF4249 at 165.235 kb on + strand, within GFF4249 at 165.237 kb on + strand, within GFF4249 at 165.237 kb on + strand, within GFF4249 at 165.238 kb on - strand, within GFF4249 at 165.248 kb on - strand, within GFF4249 at 165.330 kb on + strand, within GFF4249 at 165.330 kb on + strand, within GFF4249 at 165.331 kb on - strand, within GFF4249 at 165.331 kb on - strand, within GFF4249 at 165.331 kb on - strand, within GFF4249 at 165.331 kb on - strand, within GFF4249 at 165.382 kb on + strand at 165.382 kb on + strand at 165.382 kb on + strand at 165.383 kb on - strand at 165.383 kb on - strand at 165.383 kb on - strand at 165.419 kb on - strand at 165.994 kb on + strand, within GFF4250 at 165.994 kb on + strand, within GFF4250 at 165.994 kb on + strand, within GFF4250 at 165.994 kb on + strand, within GFF4250 at 165.994 kb on + strand, within GFF4250 at 165.994 kb on + strand, within GFF4250 at 165.995 kb on - strand, within GFF4250 at 165.995 kb on - strand, within GFF4250 at 165.995 kb on - strand, within GFF4250 at 166.238 kb on + strand at 166.238 kb on + strand at 166.238 kb on + strand at 166.239 kb on - strand at 166.239 kb on - strand at 166.437 kb on + strand, within GFF4251 at 166.437 kb on + strand, within GFF4251 at 166.437 kb on + strand, within GFF4251 at 166.437 kb on + strand, within GFF4251 at 166.438 kb on - strand, within GFF4251 at 166.438 kb on - strand, within GFF4251 at 166.438 kb on - strand, within GFF4251 at 166.438 kb on - strand, within GFF4251 at 166.438 kb on - strand, within GFF4251 at 166.438 kb on - strand, within GFF4251 at 166.438 kb on - strand, within GFF4251 at 166.620 kb on + strand, within GFF4251 at 166.620 kb on + strand, within GFF4251 at 166.621 kb on - strand, within GFF4251 at 166.621 kb on - strand, within GFF4251 at 166.773 kb on + strand, within GFF4251 at 166.774 kb on - strand, within GFF4251 at 166.785 kb on + strand, within GFF4251 at 166.785 kb on + strand, within GFF4251 at 166.785 kb on + strand, within GFF4251 at 166.785 kb on + strand, within GFF4251 at 166.785 kb on + strand, within GFF4251 at 166.786 kb on - strand, within GFF4251 at 166.786 kb on - strand, within GFF4251 at 166.893 kb on + strand at 166.893 kb on + strand at 166.894 kb on - strand at 167.044 kb on + strand at 167.044 kb on + strand at 167.045 kb on - strand at 167.045 kb on - strand at 167.101 kb on + strand at 167.101 kb on + strand at 167.101 kb on + strand at 167.102 kb on - strand at 167.102 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 164,668 + GFF4249 0.41 -0.1 164,668 + GFF4249 0.41 -0.1 164,668 + GFF4249 0.41 +0.4 164,668 + GFF4249 0.41 -1.4 164,669 - GFF4249 0.41 +0.2 164,669 - GFF4249 0.41 +0.1 164,669 - GFF4249 0.41 -0.7 164,813 - GFF4249 0.51 -0.4 164,851 + GFF4249 0.54 -0.2 164,851 + GFF4249 0.54 -0.6 164,851 + GFF4249 0.54 +0.4 164,851 + GFF4249 0.54 +0.2 164,852 - GFF4249 0.54 +0.3 164,852 - GFF4249 0.54 -1.2 164,852 - GFF4249 0.54 +0.5 164,852 - GFF4249 0.54 -1.9 164,954 - GFF4249 0.61 +0.6 164,976 + GFF4249 0.62 -1.3 165,003 + GFF4249 0.64 -2.0 165,003 + GFF4249 0.64 -0.8 165,003 + GFF4249 0.64 -2.0 165,004 - GFF4249 0.64 +0.0 165,004 - GFF4249 0.64 +1.8 165,091 + GFF4249 0.70 -0.4 165,091 + GFF4249 0.70 +0.2 165,092 - GFF4249 0.71 +0.1 165,092 - GFF4249 0.71 +0.9 165,092 - GFF4249 0.71 -0.2 165,127 + GFF4249 0.73 +0.4 165,128 - GFF4249 0.73 -0.7 165,235 + GFF4249 0.81 -1.9 165,235 + GFF4249 0.81 -0.6 165,235 + GFF4249 0.81 +1.8 165,235 + GFF4249 0.81 +2.2 165,237 + GFF4249 0.81 +1.4 165,237 + GFF4249 0.81 +0.4 165,238 - GFF4249 0.81 -0.4 165,248 - GFF4249 0.82 -0.5 165,330 + GFF4249 0.87 +0.7 165,330 + GFF4249 0.87 +0.2 165,331 - GFF4249 0.87 +0.1 165,331 - GFF4249 0.87 -0.0 165,331 - GFF4249 0.87 -1.3 165,331 - GFF4249 0.87 -0.5 165,382 + -1.1 165,382 + +0.9 165,382 + +0.8 165,383 - -0.1 165,383 - -3.0 165,383 - +0.0 165,419 - -0.9 165,994 + GFF4250 0.77 -0.6 165,994 + GFF4250 0.77 -0.7 165,994 + GFF4250 0.77 +0.1 165,994 + GFF4250 0.77 -0.7 165,994 + GFF4250 0.77 -0.9 165,994 + GFF4250 0.77 -1.2 165,995 - GFF4250 0.78 -1.4 165,995 - GFF4250 0.78 -1.7 165,995 - GFF4250 0.78 -0.5 166,238 + +0.2 166,238 + -0.8 166,238 + +0.8 166,239 - -0.6 166,239 - +0.5 166,437 + GFF4251 0.32 -0.2 166,437 + GFF4251 0.32 -0.1 166,437 + GFF4251 0.32 +1.2 166,437 + GFF4251 0.32 -0.5 166,438 - GFF4251 0.32 -0.7 166,438 - GFF4251 0.32 +0.8 166,438 - GFF4251 0.32 -2.2 166,438 - GFF4251 0.32 +0.8 166,438 - GFF4251 0.32 -1.5 166,438 - GFF4251 0.32 +0.6 166,438 - GFF4251 0.32 +0.6 166,620 + GFF4251 0.59 +0.5 166,620 + GFF4251 0.59 +0.1 166,621 - GFF4251 0.59 +0.8 166,621 - GFF4251 0.59 +0.2 166,773 + GFF4251 0.82 -0.9 166,774 - GFF4251 0.82 -0.9 166,785 + GFF4251 0.84 -2.1 166,785 + GFF4251 0.84 -1.0 166,785 + GFF4251 0.84 +0.5 166,785 + GFF4251 0.84 -0.0 166,785 + GFF4251 0.84 -1.6 166,786 - GFF4251 0.84 -0.3 166,786 - GFF4251 0.84 -0.8 166,893 + -0.0 166,893 + +0.2 166,894 - +0.8 167,044 + +0.0 167,044 + -0.0 167,045 - +0.8 167,045 - -1.6 167,101 + -0.1 167,101 + -0.8 167,101 + -0.4 167,102 - -0.5 167,102 - +0.1
Or see this region's nucleotide sequence