Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4137 and GFF4138 are separated by 46 nucleotides GFF4138 and GFF4139 are separated by 6 nucleotides
GFF4137 - Methionine ABC transporter substrate-binding protein, at 46,258 to 47,070
GFF4137
GFF4138 - ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines), at 47,117 to 48,709
GFF4138
GFF4139 - probable epoxide hydrolase, at 48,716 to 49,645
GFF4139
Position (kb) 47
48
49 Strain fitness (log2 ratio) -1
0
1 at 46.168 kb on + strand at 46.168 kb on + strand at 46.168 kb on + strand at 46.168 kb on + strand at 46.168 kb on + strand at 46.168 kb on + strand at 46.169 kb on - strand at 46.169 kb on - strand at 46.230 kb on + strand at 46.231 kb on - strand at 46.457 kb on - strand, within GFF4137 at 46.457 kb on - strand, within GFF4137 at 46.457 kb on - strand, within GFF4137 at 47.041 kb on + strand at 47.041 kb on + strand at 47.096 kb on - strand at 47.399 kb on + strand, within GFF4138 at 47.399 kb on + strand, within GFF4138 at 47.399 kb on + strand, within GFF4138 at 47.672 kb on + strand, within GFF4138 at 47.973 kb on - strand, within GFF4138 at 47.973 kb on - strand, within GFF4138 at 48.144 kb on - strand, within GFF4138 at 48.236 kb on + strand, within GFF4138 at 48.320 kb on + strand, within GFF4138 at 48.336 kb on - strand, within GFF4138 at 48.336 kb on - strand, within GFF4138 at 48.350 kb on + strand, within GFF4138 at 48.651 kb on - strand at 48.651 kb on - strand at 48.814 kb on - strand, within GFF4139 at 48.909 kb on - strand, within GFF4139 at 49.116 kb on + strand, within GFF4139 at 49.470 kb on + strand, within GFF4139 at 49.471 kb on - strand, within GFF4139
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 46,168 + +0.2 46,168 + -0.4 46,168 + +1.0 46,168 + +1.0 46,168 + -0.3 46,168 + -0.3 46,169 - -0.2 46,169 - -0.3 46,230 + +0.2 46,231 - +0.4 46,457 - GFF4137 0.24 +0.3 46,457 - GFF4137 0.24 -0.9 46,457 - GFF4137 0.24 +0.6 47,041 + +1.1 47,041 + +1.0 47,096 - +0.5 47,399 + GFF4138 0.18 -0.0 47,399 + GFF4138 0.18 -1.7 47,399 + GFF4138 0.18 -1.1 47,672 + GFF4138 0.35 -1.0 47,973 - GFF4138 0.54 +0.2 47,973 - GFF4138 0.54 -0.1 48,144 - GFF4138 0.64 +0.5 48,236 + GFF4138 0.70 -0.2 48,320 + GFF4138 0.76 -1.7 48,336 - GFF4138 0.77 -0.9 48,336 - GFF4138 0.77 +0.0 48,350 + GFF4138 0.77 +0.3 48,651 - -0.3 48,651 - -0.8 48,814 - GFF4139 0.11 +0.3 48,909 - GFF4139 0.21 -0.1 49,116 + GFF4139 0.43 +0.5 49,470 + GFF4139 0.81 +0.1 49,471 - GFF4139 0.81 -1.4
Or see this region's nucleotide sequence