Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4068 and GFF4069 overlap by 4 nucleotides GFF4069 and GFF4070 are separated by 191 nucleotides GFF4070 and GFF4071 are separated by 67 nucleotides
GFF4068 - Heavy metal sensor histidine kinase, at 283,048 to 284,445
GFF4068
GFF4069 - DNA-binding heavy metal response regulator, at 284,442 to 285,125
GFF4069
GFF4070 - no description, at 285,317 to 285,712
GFF4070
GFF4071 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 285,780 to 286,205
GFF4071
Position (kb) 284
285
286 Strain fitness (log2 ratio) -2
-1
0
1 at 283.721 kb on + strand, within GFF4068 at 283.721 kb on + strand, within GFF4068 at 283.787 kb on + strand, within GFF4068 at 283.788 kb on - strand, within GFF4068 at 283.996 kb on + strand, within GFF4068 at 283.996 kb on + strand, within GFF4068 at 283.996 kb on + strand, within GFF4068 at 283.996 kb on + strand, within GFF4068 at 283.996 kb on + strand, within GFF4068 at 283.996 kb on + strand, within GFF4068 at 283.996 kb on + strand, within GFF4068 at 283.997 kb on - strand, within GFF4068 at 284.088 kb on - strand, within GFF4068 at 284.279 kb on + strand, within GFF4068 at 284.280 kb on - strand, within GFF4068 at 284.476 kb on + strand at 284.476 kb on + strand at 284.477 kb on - strand at 284.477 kb on - strand at 284.477 kb on - strand at 284.749 kb on + strand, within GFF4069 at 284.749 kb on + strand, within GFF4069 at 284.749 kb on + strand, within GFF4069 at 284.750 kb on - strand, within GFF4069 at 284.750 kb on - strand, within GFF4069 at 284.750 kb on - strand, within GFF4069 at 284.836 kb on + strand, within GFF4069 at 284.837 kb on - strand, within GFF4069 at 285.082 kb on + strand at 285.136 kb on + strand at 285.137 kb on - strand at 285.163 kb on - strand at 285.172 kb on + strand at 285.172 kb on + strand at 285.205 kb on - strand at 285.208 kb on + strand at 285.209 kb on - strand at 285.313 kb on + strand at 285.313 kb on + strand at 285.313 kb on + strand at 285.313 kb on + strand at 285.313 kb on + strand at 285.313 kb on + strand at 285.313 kb on + strand at 285.314 kb on - strand at 285.314 kb on - strand at 285.314 kb on - strand at 285.314 kb on - strand at 285.440 kb on + strand, within GFF4070 at 285.440 kb on + strand, within GFF4070 at 285.441 kb on - strand, within GFF4070 at 285.441 kb on - strand, within GFF4070 at 285.484 kb on - strand, within GFF4070 at 285.553 kb on + strand, within GFF4070 at 285.554 kb on - strand, within GFF4070 at 285.767 kb on + strand at 285.768 kb on - strand at 286.059 kb on - strand, within GFF4071
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 283,721 + GFF4068 0.48 +0.1 283,721 + GFF4068 0.48 +0.2 283,787 + GFF4068 0.53 -0.9 283,788 - GFF4068 0.53 -0.3 283,996 + GFF4068 0.68 -2.1 283,996 + GFF4068 0.68 +0.3 283,996 + GFF4068 0.68 +0.2 283,996 + GFF4068 0.68 -0.1 283,996 + GFF4068 0.68 -0.0 283,996 + GFF4068 0.68 +1.7 283,996 + GFF4068 0.68 -0.1 283,997 - GFF4068 0.68 -1.7 284,088 - GFF4068 0.74 -0.1 284,279 + GFF4068 0.88 +0.7 284,280 - GFF4068 0.88 -0.6 284,476 + +1.2 284,476 + +0.2 284,477 - -1.1 284,477 - +0.2 284,477 - +0.8 284,749 + GFF4069 0.45 -0.6 284,749 + GFF4069 0.45 +0.2 284,749 + GFF4069 0.45 +0.3 284,750 - GFF4069 0.45 -2.0 284,750 - GFF4069 0.45 -0.3 284,750 - GFF4069 0.45 -1.1 284,836 + GFF4069 0.58 -1.5 284,837 - GFF4069 0.58 -1.1 285,082 + -0.8 285,136 + -0.8 285,137 - +1.1 285,163 - -0.7 285,172 + +0.9 285,172 + +0.4 285,205 - +0.6 285,208 + -1.2 285,209 - -0.4 285,313 + -0.6 285,313 + -0.1 285,313 + -0.2 285,313 + +0.1 285,313 + -0.2 285,313 + +0.3 285,313 + +0.4 285,314 - -0.8 285,314 - -0.6 285,314 - -2.0 285,314 - +0.2 285,440 + GFF4070 0.31 +0.8 285,440 + GFF4070 0.31 -0.1 285,441 - GFF4070 0.31 -0.1 285,441 - GFF4070 0.31 -0.7 285,484 - GFF4070 0.42 -2.6 285,553 + GFF4070 0.60 +0.1 285,554 - GFF4070 0.60 -0.8 285,767 + -0.7 285,768 - +1.0 286,059 - GFF4071 0.65 -0.7
Or see this region's nucleotide sequence