Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3983 and GFF3984 overlap by 4 nucleotides GFF3984 and GFF3985 overlap by 11 nucleotides GFF3985 and GFF3986 are separated by 4 nucleotides GFF3986 and GFF3987 are separated by 53 nucleotides
GFF3983 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate) / ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 186,602 to 188,389
GFF3983
GFF3984 - Branched-chain amino acid ABC transporter, ATP-binding protein LivF (TC 3.A.1.4.1), at 188,386 to 189,117
GFF3984
GFF3985 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 189,107 to 190,696
GFF3985
GFF3986 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 190,701 to 191,447
GFF3986
GFF3987 - 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30), at 191,501 to 192,277
GFF3987
Position (kb) 189
190
191 Strain fitness (log2 ratio) -2
-1
0
1 at 188.385 kb on + strand at 188.386 kb on - strand at 188.746 kb on + strand, within GFF3984 at 189.007 kb on + strand, within GFF3984 at 189.008 kb on - strand, within GFF3984 at 189.008 kb on - strand, within GFF3984 at 189.085 kb on + strand at 189.086 kb on - strand at 189.416 kb on + strand, within GFF3985 at 189.417 kb on - strand, within GFF3985 at 189.417 kb on - strand, within GFF3985 at 189.417 kb on - strand, within GFF3985 at 189.525 kb on + strand, within GFF3985 at 189.526 kb on - strand, within GFF3985 at 189.526 kb on - strand, within GFF3985 at 189.647 kb on + strand, within GFF3985 at 189.647 kb on + strand, within GFF3985 at 189.647 kb on + strand, within GFF3985 at 189.648 kb on - strand, within GFF3985 at 189.648 kb on - strand, within GFF3985 at 189.649 kb on + strand, within GFF3985 at 189.650 kb on - strand, within GFF3985 at 189.661 kb on + strand, within GFF3985 at 189.702 kb on - strand, within GFF3985 at 189.801 kb on - strand, within GFF3985 at 189.905 kb on + strand, within GFF3985 at 189.905 kb on + strand, within GFF3985 at 189.906 kb on - strand, within GFF3985 at 189.906 kb on - strand, within GFF3985 at 189.906 kb on - strand, within GFF3985 at 190.226 kb on + strand, within GFF3985 at 190.226 kb on + strand, within GFF3985 at 190.227 kb on - strand, within GFF3985 at 190.227 kb on - strand, within GFF3985 at 190.448 kb on + strand, within GFF3985 at 190.562 kb on + strand at 190.694 kb on + strand at 190.694 kb on + strand at 190.695 kb on - strand at 190.695 kb on - strand at 190.695 kb on - strand at 191.097 kb on - strand, within GFF3986 at 191.163 kb on + strand, within GFF3986 at 191.164 kb on - strand, within GFF3986 at 191.164 kb on - strand, within GFF3986 at 191.373 kb on + strand at 191.504 kb on + strand at 191.505 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 188,385 + -0.5 188,386 - -0.1 188,746 + GFF3984 0.49 +1.1 189,007 + GFF3984 0.85 -0.3 189,008 - GFF3984 0.85 +0.4 189,008 - GFF3984 0.85 +0.8 189,085 + +0.1 189,086 - -0.1 189,416 + GFF3985 0.19 -1.1 189,417 - GFF3985 0.19 +0.3 189,417 - GFF3985 0.19 -0.3 189,417 - GFF3985 0.19 +1.1 189,525 + GFF3985 0.26 -0.7 189,526 - GFF3985 0.26 -0.3 189,526 - GFF3985 0.26 +0.6 189,647 + GFF3985 0.34 -0.4 189,647 + GFF3985 0.34 +1.2 189,647 + GFF3985 0.34 -0.1 189,648 - GFF3985 0.34 -0.5 189,648 - GFF3985 0.34 -0.2 189,649 + GFF3985 0.34 +1.3 189,650 - GFF3985 0.34 +0.7 189,661 + GFF3985 0.35 +0.6 189,702 - GFF3985 0.37 -0.0 189,801 - GFF3985 0.44 -1.3 189,905 + GFF3985 0.50 +0.2 189,905 + GFF3985 0.50 -0.9 189,906 - GFF3985 0.50 -1.8 189,906 - GFF3985 0.50 -0.6 189,906 - GFF3985 0.50 -0.8 190,226 + GFF3985 0.70 +0.2 190,226 + GFF3985 0.70 +1.4 190,227 - GFF3985 0.70 -0.1 190,227 - GFF3985 0.70 -0.1 190,448 + GFF3985 0.84 +0.2 190,562 + -0.5 190,694 + -0.4 190,694 + -1.8 190,695 - +1.2 190,695 - -0.7 190,695 - -0.3 191,097 - GFF3986 0.53 -0.9 191,163 + GFF3986 0.62 -0.3 191,164 - GFF3986 0.62 -1.1 191,164 - GFF3986 0.62 -0.3 191,373 + -0.3 191,504 + -1.9 191,505 - -0.3
Or see this region's nucleotide sequence