Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3873 and GFF3874 are separated by 13 nucleotides GFF3874 and GFF3875 overlap by 4 nucleotides GFF3875 and GFF3876 are separated by 17 nucleotides GFF3876 and GFF3877 overlap by 1 nucleotides
GFF3873 - Pyridoxine 5'-phosphate synthase (EC 2.6.99.2), at 71,073 to 71,834
GFF3873
GFF3874 - Holo-[acyl-carrier-protein] synthase (EC 2.7.8.7), at 71,848 to 72,240
GFF3874
GFF3875 - beta-N-acetylglucosaminidase (EC 3.2.1.52), at 72,237 to 73,319
GFF3875
GFF3876 - UTP pyrophosphatase (EC 3.6.1.9), at 73,337 to 73,852
GFF3876
GFF3877 - Uncharacterized chaperone protein YegD, at 73,852 to 75,132
GFF3877
Position (kb)
72
73
74 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 71.854 kb on + strand at 71.854 kb on + strand at 71.854 kb on + strand at 71.855 kb on - strand at 71.856 kb on - strand at 72.004 kb on + strand, within GFF3874 at 72.004 kb on + strand, within GFF3874 at 72.005 kb on - strand, within GFF3874 at 72.005 kb on - strand, within GFF3874 at 72.205 kb on + strand at 72.235 kb on + strand at 72.236 kb on + strand at 72.236 kb on + strand at 72.236 kb on + strand at 72.236 kb on + strand at 72.236 kb on + strand at 72.236 kb on + strand at 72.236 kb on + strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.861 kb on + strand, within GFF3875 at 72.861 kb on + strand, within GFF3875 at 72.861 kb on + strand, within GFF3875 at 72.862 kb on - strand, within GFF3875 at 72.912 kb on + strand, within GFF3875 at 72.913 kb on - strand, within GFF3875 at 73.035 kb on + strand, within GFF3875 at 73.036 kb on - strand, within GFF3875 at 73.036 kb on - strand, within GFF3875 at 73.036 kb on - strand, within GFF3875 at 73.036 kb on - strand, within GFF3875 at 73.036 kb on - strand, within GFF3875 at 73.059 kb on + strand, within GFF3875 at 73.059 kb on + strand, within GFF3875 at 73.060 kb on - strand, within GFF3875 at 73.284 kb on + strand at 73.284 kb on + strand at 73.285 kb on - strand at 73.392 kb on + strand, within GFF3876 at 73.392 kb on + strand, within GFF3876 at 73.392 kb on + strand, within GFF3876 at 73.392 kb on + strand, within GFF3876 at 73.393 kb on - strand, within GFF3876 at 73.393 kb on - strand, within GFF3876 at 73.393 kb on - strand, within GFF3876 at 73.422 kb on + strand, within GFF3876 at 73.422 kb on + strand, within GFF3876 at 73.423 kb on - strand, within GFF3876 at 73.423 kb on - strand, within GFF3876 at 73.452 kb on + strand, within GFF3876 at 73.452 kb on + strand, within GFF3876 at 73.452 kb on + strand, within GFF3876 at 73.452 kb on + strand, within GFF3876 at 73.452 kb on + strand, within GFF3876 at 73.452 kb on + strand, within GFF3876 at 73.452 kb on + strand, within GFF3876 at 73.452 kb on + strand, within GFF3876 at 73.452 kb on + strand, within GFF3876 at 73.452 kb on + strand, within GFF3876 at 73.452 kb on + strand, within GFF3876 at 73.453 kb on - strand, within GFF3876 at 73.453 kb on - strand, within GFF3876 at 73.453 kb on - strand, within GFF3876 at 73.453 kb on - strand, within GFF3876 at 73.453 kb on - strand, within GFF3876 at 73.453 kb on - strand, within GFF3876 at 73.453 kb on - strand, within GFF3876 at 73.453 kb on - strand, within GFF3876 at 73.453 kb on - strand, within GFF3876 at 73.453 kb on - strand, within GFF3876 at 73.453 kb on - strand, within GFF3876 at 73.453 kb on - strand, within GFF3876 at 73.529 kb on + strand, within GFF3876 at 73.529 kb on + strand, within GFF3876 at 73.529 kb on + strand, within GFF3876 at 73.529 kb on + strand, within GFF3876 at 73.529 kb on + strand, within GFF3876 at 73.530 kb on - strand, within GFF3876 at 73.530 kb on - strand, within GFF3876 at 73.530 kb on - strand, within GFF3876 at 73.631 kb on + strand, within GFF3876 at 73.632 kb on - strand, within GFF3876 at 73.632 kb on - strand, within GFF3876 at 73.632 kb on - strand, within GFF3876 at 73.632 kb on - strand, within GFF3876 at 73.655 kb on + strand, within GFF3876 at 73.655 kb on + strand, within GFF3876 at 73.656 kb on - strand, within GFF3876 at 73.710 kb on + strand, within GFF3876 at 73.710 kb on + strand, within GFF3876 at 73.710 kb on + strand, within GFF3876 at 73.710 kb on + strand, within GFF3876 at 73.710 kb on + strand, within GFF3876 at 73.710 kb on + strand, within GFF3876 at 73.710 kb on + strand, within GFF3876 at 73.710 kb on + strand, within GFF3876 at 73.710 kb on + strand, within GFF3876 at 73.710 kb on + strand, within GFF3876 at 73.711 kb on - strand, within GFF3876 at 73.711 kb on - strand, within GFF3876 at 73.711 kb on - strand, within GFF3876 at 73.711 kb on - strand, within GFF3876 at 73.711 kb on - strand, within GFF3876 at 73.711 kb on - strand, within GFF3876 at 73.711 kb on - strand, within GFF3876 at 73.711 kb on - strand, within GFF3876 at 73.711 kb on - strand, within GFF3876 at 73.711 kb on - strand, within GFF3876 at 73.716 kb on + strand, within GFF3876 at 73.716 kb on + strand, within GFF3876 at 73.716 kb on + strand, within GFF3876 at 73.716 kb on + strand, within GFF3876 at 73.716 kb on + strand, within GFF3876 at 73.717 kb on - strand, within GFF3876 at 73.717 kb on - strand, within GFF3876 at 73.717 kb on - strand, within GFF3876 at 73.717 kb on - strand, within GFF3876 at 73.717 kb on - strand, within GFF3876 at 73.845 kb on + strand at 73.845 kb on + strand at 73.846 kb on - strand at 73.846 kb on - strand at 73.846 kb on - strand at 73.846 kb on - strand at 73.846 kb on - strand at 74.000 kb on + strand, within GFF3877 at 74.000 kb on + strand, within GFF3877 at 74.001 kb on - strand, within GFF3877 at 74.001 kb on - strand, within GFF3877 at 74.001 kb on - strand, within GFF3877 at 74.001 kb on - strand, within GFF3877 at 74.001 kb on - strand, within GFF3877 at 74.001 kb on - strand, within GFF3877 at 74.276 kb on + strand, within GFF3877 at 74.276 kb on + strand, within GFF3877 at 74.277 kb on - strand, within GFF3877 at 74.277 kb on - strand, within GFF3877 at 74.277 kb on - strand, within GFF3877
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 71,854 + -0.5 71,854 + -0.4 71,854 + -0.4 71,855 - -0.1 71,856 - -0.7 72,004 + GFF3874 0.40 +0.4 72,004 + GFF3874 0.40 +0.1 72,005 - GFF3874 0.40 -1.9 72,005 - GFF3874 0.40 +0.8 72,205 + +0.9 72,235 + -0.9 72,236 + +0.8 72,236 + -0.1 72,236 + -1.9 72,236 + +0.1 72,236 + -1.7 72,236 + +0.0 72,236 + -0.0 72,237 - +0.5 72,237 - -0.5 72,237 - +0.9 72,237 - -0.8 72,237 - +0.2 72,237 - -0.2 72,237 - -1.1 72,237 - +0.1 72,237 - -0.5 72,237 - -0.9 72,237 - -0.3 72,237 - -0.1 72,237 - -2.2 72,861 + GFF3875 0.58 -0.4 72,861 + GFF3875 0.58 +0.5 72,861 + GFF3875 0.58 -2.4 72,862 - GFF3875 0.58 -0.8 72,912 + GFF3875 0.62 -0.0 72,913 - GFF3875 0.62 -0.7 73,035 + GFF3875 0.74 -1.2 73,036 - GFF3875 0.74 +0.3 73,036 - GFF3875 0.74 -0.8 73,036 - GFF3875 0.74 +0.3 73,036 - GFF3875 0.74 -1.2 73,036 - GFF3875 0.74 -0.7 73,059 + GFF3875 0.76 +0.7 73,059 + GFF3875 0.76 -0.9 73,060 - GFF3875 0.76 -1.6 73,284 + +0.1 73,284 + -0.7 73,285 - +0.3 73,392 + GFF3876 0.11 +0.2 73,392 + GFF3876 0.11 -1.4 73,392 + GFF3876 0.11 -0.0 73,392 + GFF3876 0.11 -0.9 73,393 - GFF3876 0.11 -0.8 73,393 - GFF3876 0.11 +0.3 73,393 - GFF3876 0.11 -1.0 73,422 + GFF3876 0.16 +1.1 73,422 + GFF3876 0.16 -0.4 73,423 - GFF3876 0.17 -0.7 73,423 - GFF3876 0.17 +0.2 73,452 + GFF3876 0.22 +0.4 73,452 + GFF3876 0.22 +0.6 73,452 + GFF3876 0.22 -0.2 73,452 + GFF3876 0.22 -0.9 73,452 + GFF3876 0.22 +2.0 73,452 + GFF3876 0.22 -1.6 73,452 + GFF3876 0.22 -0.3 73,452 + GFF3876 0.22 +0.1 73,452 + GFF3876 0.22 +0.2 73,452 + GFF3876 0.22 -0.3 73,452 + GFF3876 0.22 +0.1 73,453 - GFF3876 0.22 -0.3 73,453 - GFF3876 0.22 -0.7 73,453 - GFF3876 0.22 +0.7 73,453 - GFF3876 0.22 +0.7 73,453 - GFF3876 0.22 +1.5 73,453 - GFF3876 0.22 +1.4 73,453 - GFF3876 0.22 -1.7 73,453 - GFF3876 0.22 -0.9 73,453 - GFF3876 0.22 -2.6 73,453 - GFF3876 0.22 +0.2 73,453 - GFF3876 0.22 +0.5 73,453 - GFF3876 0.22 +0.2 73,529 + GFF3876 0.37 +1.0 73,529 + GFF3876 0.37 +1.2 73,529 + GFF3876 0.37 -0.9 73,529 + GFF3876 0.37 +0.4 73,529 + GFF3876 0.37 -0.1 73,530 - GFF3876 0.37 +1.4 73,530 - GFF3876 0.37 +0.1 73,530 - GFF3876 0.37 -0.0 73,631 + GFF3876 0.57 -0.3 73,632 - GFF3876 0.57 +1.2 73,632 - GFF3876 0.57 -1.8 73,632 - GFF3876 0.57 +0.1 73,632 - GFF3876 0.57 -2.5 73,655 + GFF3876 0.62 -1.9 73,655 + GFF3876 0.62 -0.5 73,656 - GFF3876 0.62 +0.2 73,710 + GFF3876 0.72 +0.4 73,710 + GFF3876 0.72 -1.1 73,710 + GFF3876 0.72 +1.4 73,710 + GFF3876 0.72 +0.1 73,710 + GFF3876 0.72 +0.7 73,710 + GFF3876 0.72 -0.4 73,710 + GFF3876 0.72 +1.0 73,710 + GFF3876 0.72 -0.9 73,710 + GFF3876 0.72 -0.4 73,710 + GFF3876 0.72 +0.4 73,711 - GFF3876 0.72 +0.4 73,711 - GFF3876 0.72 +0.7 73,711 - GFF3876 0.72 -1.3 73,711 - GFF3876 0.72 -1.2 73,711 - GFF3876 0.72 -0.1 73,711 - GFF3876 0.72 +0.3 73,711 - GFF3876 0.72 +0.4 73,711 - GFF3876 0.72 +0.3 73,711 - GFF3876 0.72 -0.3 73,711 - GFF3876 0.72 +1.1 73,716 + GFF3876 0.73 +0.1 73,716 + GFF3876 0.73 -0.6 73,716 + GFF3876 0.73 +0.3 73,716 + GFF3876 0.73 -0.7 73,716 + GFF3876 0.73 +0.2 73,717 - GFF3876 0.74 -0.4 73,717 - GFF3876 0.74 -0.3 73,717 - GFF3876 0.74 -0.6 73,717 - GFF3876 0.74 +0.1 73,717 - GFF3876 0.74 -1.8 73,845 + +0.3 73,845 + -1.4 73,846 - -0.7 73,846 - +0.5 73,846 - +0.6 73,846 - +0.1 73,846 - +0.5 74,000 + GFF3877 0.12 +0.1 74,000 + GFF3877 0.12 -2.1 74,001 - GFF3877 0.12 -0.5 74,001 - GFF3877 0.12 -0.1 74,001 - GFF3877 0.12 +0.5 74,001 - GFF3877 0.12 -0.7 74,001 - GFF3877 0.12 +0.9 74,001 - GFF3877 0.12 -0.9 74,276 + GFF3877 0.33 +0.1 74,276 + GFF3877 0.33 +0.5 74,277 - GFF3877 0.33 -0.5 74,277 - GFF3877 0.33 -1.5 74,277 - GFF3877 0.33 +0.8
Or see this region's nucleotide sequence