Strain Fitness in Variovorax sp. SCN45 around GFF3841

Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3839 and GFF3840 are separated by 16 nucleotidesGFF3840 and GFF3841 overlap by 4 nucleotidesGFF3841 and GFF3842 are separated by 7 nucleotides GFF3839 - Porphobilinogen deaminase (EC 2.5.1.61), at 37,525 to 38,418 GFF3839 GFF3840 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 38,435 to 39,205 GFF3840 GFF3841 - Uncharacterized protein EC-HemX, at 39,202 to 40,296 GFF3841 GFF3842 - Uncharacterized protein EC-HemY in Proteobacteria (unrelated to HemY-type PPO in GramPositives), at 40,304 to 41,587 GFF3842 Position (kb) 39 40 41Strain fitness (log2 ratio) -3 -2 -1 0 1at 39.189 kb on + strandat 39.189 kb on + strandat 39.189 kb on + strandat 39.189 kb on + strandat 39.189 kb on + strandat 39.189 kb on + strandat 39.190 kb on - strandat 39.190 kb on - strandat 39.190 kb on - strandat 39.190 kb on - strandat 39.190 kb on - strandat 39.378 kb on + strand, within GFF3841at 40.042 kb on + strand, within GFF3841at 40.042 kb on + strand, within GFF3841at 40.042 kb on + strand, within GFF3841at 40.042 kb on + strand, within GFF3841at 40.042 kb on + strand, within GFF3841at 40.042 kb on + strand, within GFF3841at 40.042 kb on + strand, within GFF3841at 40.042 kb on + strand, within GFF3841at 40.042 kb on + strand, within GFF3841at 40.042 kb on + strand, within GFF3841at 40.043 kb on - strand, within GFF3841at 40.043 kb on - strand, within GFF3841at 40.043 kb on - strand, within GFF3841at 40.043 kb on - strand, within GFF3841at 40.043 kb on - strand, within GFF3841at 40.043 kb on - strand, within GFF3841at 40.043 kb on - strand, within GFF3841at 40.043 kb on - strand, within GFF3841at 40.159 kb on + strand, within GFF3841at 40.160 kb on - strand, within GFF3841at 40.160 kb on - strand, within GFF3841at 40.160 kb on - strand, within GFF3841

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
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39,189 + +0.4
39,189 + -1.5
39,189 + -0.5
39,189 + +0.1
39,189 + -0.5
39,189 + -1.0
39,190 - -2.1
39,190 - +0.5
39,190 - +1.7
39,190 - +0.7
39,190 - -0.4
39,378 + GFF3841 0.16 -0.8
40,042 + GFF3841 0.77 +0.8
40,042 + GFF3841 0.77 -2.2
40,042 + GFF3841 0.77 -0.7
40,042 + GFF3841 0.77 -2.3
40,042 + GFF3841 0.77 -0.3
40,042 + GFF3841 0.77 -0.7
40,042 + GFF3841 0.77 -0.5
40,042 + GFF3841 0.77 -0.4
40,042 + GFF3841 0.77 -0.1
40,042 + GFF3841 0.77 -1.1
40,043 - GFF3841 0.77 +0.3
40,043 - GFF3841 0.77 -0.8
40,043 - GFF3841 0.77 -1.5
40,043 - GFF3841 0.77 -1.1
40,043 - GFF3841 0.77 -0.4
40,043 - GFF3841 0.77 -0.3
40,043 - GFF3841 0.77 -0.0
40,043 - GFF3841 0.77 -2.9
40,159 + GFF3841 0.87 -2.1
40,160 - GFF3841 0.87 -0.5
40,160 - GFF3841 0.87 -1.7
40,160 - GFF3841 0.87 -0.8

Or see this region's nucleotide sequence