Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3836 and GFF3837 overlap by 4 nucleotides GFF3837 and GFF3838 are separated by 166 nucleotides GFF3838 and GFF3839 are separated by 115 nucleotides
GFF3836 - Cyclic beta-1,2-glucan synthase (EC 2.4.1.-), at 26,019 to 34,184
GFF3836
GFF3837 - no description, at 34,181 to 34,399
GFF3837
GFF3838 - Phosphoenolpyruvate carboxylase (EC 4.1.1.31), at 34,566 to 37,409
GFF3838
GFF3839 - Porphobilinogen deaminase (EC 2.5.1.61), at 37,525 to 38,418
GFF3839
Position (kb) 34
35
36
37
38 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 33.612 kb on + strand at 33.612 kb on + strand at 33.613 kb on - strand at 33.676 kb on + strand at 33.736 kb on + strand at 33.736 kb on + strand at 33.736 kb on + strand at 33.737 kb on + strand at 33.737 kb on - strand at 33.737 kb on - strand at 33.737 kb on - strand at 33.737 kb on - strand at 33.754 kb on + strand at 33.755 kb on - strand at 33.755 kb on - strand at 33.755 kb on - strand at 33.755 kb on - strand at 33.809 kb on - strand at 33.811 kb on + strand at 33.811 kb on + strand at 33.811 kb on + strand at 33.811 kb on + strand at 33.811 kb on + strand at 33.812 kb on - strand at 33.812 kb on - strand at 33.812 kb on - strand at 33.812 kb on - strand at 33.901 kb on + strand at 33.902 kb on - strand at 33.902 kb on - strand at 33.902 kb on - strand at 33.902 kb on - strand at 33.902 kb on - strand at 33.932 kb on - strand at 34.378 kb on + strand at 34.416 kb on + strand at 34.476 kb on + strand at 34.477 kb on - strand at 34.477 kb on - strand at 34.486 kb on + strand at 34.487 kb on - strand at 34.515 kb on + strand at 34.516 kb on - strand at 34.516 kb on - strand at 34.516 kb on - strand at 34.699 kb on + strand at 34.699 kb on + strand at 34.699 kb on + strand at 34.700 kb on - strand at 34.700 kb on - strand at 34.862 kb on - strand, within GFF3838 at 34.904 kb on - strand, within GFF3838 at 34.945 kb on + strand, within GFF3838 at 34.945 kb on + strand, within GFF3838 at 34.945 kb on + strand, within GFF3838 at 34.945 kb on + strand, within GFF3838 at 34.946 kb on - strand, within GFF3838 at 34.946 kb on - strand, within GFF3838 at 34.946 kb on - strand, within GFF3838 at 35.188 kb on + strand, within GFF3838 at 35.188 kb on + strand, within GFF3838 at 35.189 kb on - strand, within GFF3838 at 35.251 kb on - strand, within GFF3838 at 35.371 kb on + strand, within GFF3838 at 35.371 kb on + strand, within GFF3838 at 35.455 kb on + strand, within GFF3838 at 35.456 kb on - strand, within GFF3838 at 35.456 kb on - strand, within GFF3838 at 35.456 kb on - strand, within GFF3838 at 35.578 kb on + strand, within GFF3838 at 35.579 kb on - strand, within GFF3838 at 35.630 kb on - strand, within GFF3838 at 35.695 kb on + strand, within GFF3838 at 35.696 kb on - strand, within GFF3838 at 35.696 kb on - strand, within GFF3838 at 35.872 kb on + strand, within GFF3838 at 35.873 kb on - strand, within GFF3838 at 35.873 kb on - strand, within GFF3838 at 35.873 kb on - strand, within GFF3838 at 35.959 kb on + strand, within GFF3838 at 35.959 kb on + strand, within GFF3838 at 35.960 kb on - strand, within GFF3838 at 36.046 kb on + strand, within GFF3838 at 36.046 kb on + strand, within GFF3838 at 36.047 kb on - strand, within GFF3838 at 36.047 kb on - strand, within GFF3838 at 36.047 kb on - strand, within GFF3838 at 36.266 kb on - strand, within GFF3838 at 36.344 kb on - strand, within GFF3838 at 36.478 kb on + strand, within GFF3838 at 36.478 kb on + strand, within GFF3838 at 36.478 kb on + strand, within GFF3838 at 36.479 kb on - strand, within GFF3838 at 36.479 kb on - strand, within GFF3838 at 36.496 kb on + strand, within GFF3838 at 36.496 kb on + strand, within GFF3838 at 36.496 kb on + strand, within GFF3838 at 36.496 kb on + strand, within GFF3838 at 36.497 kb on - strand, within GFF3838 at 36.497 kb on - strand, within GFF3838 at 36.497 kb on - strand, within GFF3838 at 36.497 kb on - strand, within GFF3838 at 36.665 kb on - strand, within GFF3838 at 36.665 kb on - strand, within GFF3838 at 36.665 kb on - strand, within GFF3838 at 36.700 kb on + strand, within GFF3838 at 36.700 kb on + strand, within GFF3838 at 36.700 kb on + strand, within GFF3838 at 36.701 kb on - strand, within GFF3838 at 36.701 kb on - strand, within GFF3838 at 36.703 kb on + strand, within GFF3838 at 36.703 kb on + strand, within GFF3838 at 36.703 kb on + strand, within GFF3838 at 36.870 kb on + strand, within GFF3838 at 36.973 kb on + strand, within GFF3838 at 36.973 kb on + strand, within GFF3838 at 36.973 kb on + strand, within GFF3838 at 36.973 kb on + strand, within GFF3838 at 36.973 kb on + strand, within GFF3838 at 36.973 kb on + strand, within GFF3838 at 36.973 kb on + strand, within GFF3838 at 36.973 kb on + strand, within GFF3838 at 36.973 kb on + strand, within GFF3838 at 36.973 kb on + strand, within GFF3838 at 36.973 kb on + strand, within GFF3838 at 36.973 kb on + strand, within GFF3838 at 36.973 kb on + strand, within GFF3838 at 36.973 kb on + strand, within GFF3838 at 36.974 kb on - strand, within GFF3838 at 36.974 kb on - strand, within GFF3838 at 36.974 kb on - strand, within GFF3838 at 36.974 kb on - strand, within GFF3838 at 36.974 kb on - strand, within GFF3838 at 36.974 kb on - strand, within GFF3838 at 36.974 kb on - strand, within GFF3838 at 36.974 kb on - strand, within GFF3838 at 36.974 kb on - strand, within GFF3838 at 36.974 kb on - strand, within GFF3838 at 36.974 kb on - strand, within GFF3838 at 36.974 kb on - strand, within GFF3838 at 36.974 kb on - strand, within GFF3838 at 36.974 kb on - strand, within GFF3838 at 36.974 kb on - strand, within GFF3838 at 36.974 kb on - strand, within GFF3838 at 36.974 kb on - strand, within GFF3838 at 36.974 kb on - strand, within GFF3838 at 36.974 kb on - strand, within GFF3838 at 37.141 kb on + strand at 37.141 kb on + strand at 37.142 kb on - strand at 37.142 kb on - strand at 37.142 kb on - strand at 37.142 kb on - strand at 37.142 kb on - strand at 37.142 kb on - strand at 37.279 kb on + strand at 37.279 kb on + strand at 37.280 kb on - strand at 37.280 kb on - strand at 37.280 kb on - strand at 37.280 kb on - strand at 37.280 kb on - strand at 37.341 kb on - strand at 37.496 kb on + strand at 37.496 kb on + strand at 37.496 kb on + strand at 37.496 kb on + strand at 37.496 kb on + strand at 37.496 kb on + strand at 37.496 kb on + strand at 37.496 kb on + strand at 37.496 kb on + strand at 37.497 kb on - strand at 37.497 kb on - strand at 37.497 kb on - strand at 37.497 kb on - strand at 37.497 kb on - strand at 37.498 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 33,612 + -0.3 33,612 + -0.9 33,613 - +1.7 33,676 + -0.2 33,736 + -0.4 33,736 + +0.5 33,736 + -0.3 33,737 + -0.4 33,737 - -0.1 33,737 - -0.8 33,737 - -0.9 33,737 - -0.2 33,754 + +0.0 33,755 - -1.2 33,755 - +1.1 33,755 - -0.2 33,755 - +0.1 33,809 - -1.8 33,811 + -1.8 33,811 + -0.8 33,811 + +0.2 33,811 + +0.1 33,811 + -1.0 33,812 - +0.1 33,812 - -0.2 33,812 - -2.1 33,812 - -0.9 33,901 + -1.2 33,902 - -0.5 33,902 - -0.4 33,902 - -0.8 33,902 - -0.7 33,902 - -0.2 33,932 - -0.8 34,378 + -0.3 34,416 + +0.4 34,476 + +0.1 34,477 - +1.7 34,477 - -0.9 34,486 + -0.3 34,487 - -0.1 34,515 + -0.9 34,516 - -0.1 34,516 - -0.8 34,516 - -2.4 34,699 + -2.3 34,699 + +1.7 34,699 + -1.2 34,700 - -1.1 34,700 - -2.2 34,862 - GFF3838 0.10 -1.7 34,904 - GFF3838 0.12 -2.1 34,945 + GFF3838 0.13 -1.5 34,945 + GFF3838 0.13 -1.4 34,945 + GFF3838 0.13 -0.9 34,945 + GFF3838 0.13 -1.1 34,946 - GFF3838 0.13 -0.7 34,946 - GFF3838 0.13 -0.4 34,946 - GFF3838 0.13 -2.3 35,188 + GFF3838 0.22 -1.1 35,188 + GFF3838 0.22 -4.8 35,189 - GFF3838 0.22 -0.1 35,251 - GFF3838 0.24 -0.7 35,371 + GFF3838 0.28 -3.0 35,371 + GFF3838 0.28 -1.3 35,455 + GFF3838 0.31 -1.0 35,456 - GFF3838 0.31 -0.9 35,456 - GFF3838 0.31 -1.3 35,456 - GFF3838 0.31 -1.3 35,578 + GFF3838 0.36 -1.9 35,579 - GFF3838 0.36 -2.7 35,630 - GFF3838 0.37 -0.3 35,695 + GFF3838 0.40 -1.3 35,696 - GFF3838 0.40 -0.9 35,696 - GFF3838 0.40 -2.9 35,872 + GFF3838 0.46 -2.9 35,873 - GFF3838 0.46 -2.0 35,873 - GFF3838 0.46 -0.1 35,873 - GFF3838 0.46 -3.0 35,959 + GFF3838 0.49 -1.9 35,959 + GFF3838 0.49 +0.7 35,960 - GFF3838 0.49 -0.9 36,046 + GFF3838 0.52 -0.7 36,046 + GFF3838 0.52 -0.9 36,047 - GFF3838 0.52 -3.0 36,047 - GFF3838 0.52 -0.8 36,047 - GFF3838 0.52 -1.9 36,266 - GFF3838 0.60 -4.2 36,344 - GFF3838 0.63 -0.8 36,478 + GFF3838 0.67 -1.3 36,478 + GFF3838 0.67 -1.6 36,478 + GFF3838 0.67 -1.5 36,479 - GFF3838 0.67 -2.9 36,479 - GFF3838 0.67 -1.2 36,496 + GFF3838 0.68 -0.1 36,496 + GFF3838 0.68 -2.4 36,496 + GFF3838 0.68 +0.7 36,496 + GFF3838 0.68 -2.0 36,497 - GFF3838 0.68 -0.1 36,497 - GFF3838 0.68 -2.7 36,497 - GFF3838 0.68 -3.9 36,497 - GFF3838 0.68 -0.8 36,665 - GFF3838 0.74 -2.9 36,665 - GFF3838 0.74 -0.9 36,665 - GFF3838 0.74 -1.0 36,700 + GFF3838 0.75 -3.9 36,700 + GFF3838 0.75 -2.1 36,700 + GFF3838 0.75 -3.5 36,701 - GFF3838 0.75 -0.9 36,701 - GFF3838 0.75 -1.4 36,703 + GFF3838 0.75 -1.1 36,703 + GFF3838 0.75 -1.4 36,703 + GFF3838 0.75 -2.7 36,870 + GFF3838 0.81 -1.1 36,973 + GFF3838 0.85 +0.7 36,973 + GFF3838 0.85 -0.7 36,973 + GFF3838 0.85 -2.0 36,973 + GFF3838 0.85 -1.5 36,973 + GFF3838 0.85 -2.3 36,973 + GFF3838 0.85 -1.7 36,973 + GFF3838 0.85 -2.0 36,973 + GFF3838 0.85 -1.6 36,973 + GFF3838 0.85 +1.7 36,973 + GFF3838 0.85 -1.4 36,973 + GFF3838 0.85 -1.5 36,973 + GFF3838 0.85 -2.6 36,973 + GFF3838 0.85 -2.4 36,973 + GFF3838 0.85 -1.8 36,974 - GFF3838 0.85 -2.9 36,974 - GFF3838 0.85 -1.3 36,974 - GFF3838 0.85 -2.1 36,974 - GFF3838 0.85 -2.1 36,974 - GFF3838 0.85 -0.7 36,974 - GFF3838 0.85 -1.1 36,974 - GFF3838 0.85 -1.2 36,974 - GFF3838 0.85 -1.3 36,974 - GFF3838 0.85 -2.2 36,974 - GFF3838 0.85 +0.1 36,974 - GFF3838 0.85 -1.7 36,974 - GFF3838 0.85 -2.2 36,974 - GFF3838 0.85 -2.3 36,974 - GFF3838 0.85 -0.9 36,974 - GFF3838 0.85 -1.9 36,974 - GFF3838 0.85 -4.0 36,974 - GFF3838 0.85 -1.1 36,974 - GFF3838 0.85 -1.6 36,974 - GFF3838 0.85 -2.7 37,141 + -2.8 37,141 + -1.7 37,142 - -1.6 37,142 - -3.1 37,142 - -2.1 37,142 - -2.4 37,142 - -2.4 37,142 - -3.5 37,279 + -0.9 37,279 + -3.2 37,280 - -1.2 37,280 - -1.7 37,280 - +3.7 37,280 - -1.8 37,280 - -2.4 37,341 - -0.9 37,496 + -1.7 37,496 + +0.5 37,496 + +0.1 37,496 + -0.3 37,496 + +0.2 37,496 + +1.4 37,496 + -1.4 37,496 + -1.7 37,496 + +0.1 37,497 - -0.5 37,497 - +0.4 37,497 - -0.5 37,497 - -0.5 37,497 - -0.1 37,498 - -0.9
Or see this region's nucleotide sequence