Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3747 and GFF3748 are separated by 7 nucleotides GFF3748 and GFF3749 are separated by 72 nucleotides GFF3749 and GFF3750 overlap by 4 nucleotides GFF3750 and GFF3751 are separated by 46 nucleotides
GFF3747 - putative esterase, at 242,411 to 243,253
GFF3747
GFF3748 - KEGG: dihydrodipicolinate synthase , at 243,261 to 244,118
GFF3748
GFF3749 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 244,191 to 244,997
GFF3749
GFF3750 - no description, at 244,994 to 245,395
GFF3750
GFF3751 - Hydroxymethylpyrimidine ABC transporter, transmembrane component, at 245,442 to 246,209
GFF3751
Position (kb) 244
245 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 243.210 kb on + strand at 243.210 kb on + strand at 243.210 kb on + strand at 243.210 kb on + strand at 243.211 kb on - strand at 243.211 kb on - strand at 243.487 kb on + strand, within GFF3748 at 243.487 kb on + strand, within GFF3748 at 243.487 kb on + strand, within GFF3748 at 243.487 kb on + strand, within GFF3748 at 243.487 kb on + strand, within GFF3748 at 243.487 kb on + strand, within GFF3748 at 243.487 kb on + strand, within GFF3748 at 243.488 kb on - strand, within GFF3748 at 243.488 kb on - strand, within GFF3748 at 243.488 kb on - strand, within GFF3748 at 243.488 kb on - strand, within GFF3748 at 243.488 kb on - strand, within GFF3748 at 243.488 kb on - strand, within GFF3748 at 243.488 kb on - strand, within GFF3748 at 243.622 kb on + strand, within GFF3748 at 243.745 kb on + strand, within GFF3748 at 243.745 kb on + strand, within GFF3748 at 243.746 kb on - strand, within GFF3748 at 243.746 kb on - strand, within GFF3748 at 243.788 kb on - strand, within GFF3748 at 243.788 kb on - strand, within GFF3748 at 243.788 kb on - strand, within GFF3748 at 243.826 kb on + strand, within GFF3748 at 243.826 kb on + strand, within GFF3748 at 243.827 kb on - strand, within GFF3748 at 243.923 kb on - strand, within GFF3748 at 243.923 kb on - strand, within GFF3748 at 244.051 kb on + strand at 244.051 kb on + strand at 244.051 kb on + strand at 244.051 kb on + strand at 244.051 kb on + strand at 244.052 kb on - strand at 244.052 kb on - strand at 244.052 kb on - strand at 244.052 kb on - strand at 244.052 kb on - strand at 244.052 kb on - strand at 244.052 kb on - strand at 244.143 kb on - strand at 244.228 kb on + strand at 244.229 kb on - strand at 244.558 kb on + strand, within GFF3749 at 245.070 kb on + strand, within GFF3750 at 245.071 kb on - strand, within GFF3750 at 245.071 kb on - strand, within GFF3750 at 245.071 kb on - strand, within GFF3750 at 245.073 kb on + strand, within GFF3750 at 245.073 kb on + strand, within GFF3750 at 245.073 kb on + strand, within GFF3750 at 245.074 kb on - strand, within GFF3750 at 245.232 kb on + strand, within GFF3750 at 245.232 kb on + strand, within GFF3750 at 245.233 kb on - strand, within GFF3750 at 245.233 kb on - strand, within GFF3750 at 245.233 kb on - strand, within GFF3750 at 245.233 kb on - strand, within GFF3750 at 245.233 kb on - strand, within GFF3750 at 245.233 kb on - strand, within GFF3750 at 245.271 kb on + strand, within GFF3750 at 245.271 kb on + strand, within GFF3750 at 245.271 kb on + strand, within GFF3750 at 245.271 kb on + strand, within GFF3750 at 245.271 kb on + strand, within GFF3750 at 245.272 kb on - strand, within GFF3750 at 245.272 kb on - strand, within GFF3750 at 245.272 kb on - strand, within GFF3750 at 245.272 kb on - strand, within GFF3750 at 245.272 kb on - strand, within GFF3750 at 245.272 kb on - strand, within GFF3750 at 245.334 kb on + strand, within GFF3750 at 245.334 kb on + strand, within GFF3750 at 245.334 kb on + strand, within GFF3750 at 245.335 kb on - strand, within GFF3750 at 245.335 kb on - strand, within GFF3750 at 245.335 kb on - strand, within GFF3750 at 245.384 kb on + strand at 245.384 kb on + strand at 245.385 kb on - strand at 245.385 kb on - strand at 245.385 kb on - strand at 245.385 kb on - strand at 245.419 kb on + strand at 245.419 kb on + strand at 245.420 kb on - strand at 245.491 kb on + strand at 245.491 kb on + strand at 245.491 kb on + strand at 245.492 kb on - strand at 245.492 kb on - strand at 245.492 kb on - strand at 245.492 kb on - strand at 245.492 kb on - strand at 245.539 kb on + strand, within GFF3751 at 245.539 kb on + strand, within GFF3751 at 245.540 kb on - strand, within GFF3751 at 245.689 kb on + strand, within GFF3751 at 245.689 kb on + strand, within GFF3751 at 245.689 kb on + strand, within GFF3751 at 245.689 kb on + strand, within GFF3751 at 245.690 kb on - strand, within GFF3751 at 245.690 kb on - strand, within GFF3751 at 245.690 kb on - strand, within GFF3751 at 245.690 kb on - strand, within GFF3751 at 245.690 kb on - strand, within GFF3751 at 245.690 kb on - strand, within GFF3751 at 245.914 kb on + strand, within GFF3751
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 243,210 + -0.3 243,210 + -1.5 243,210 + -1.8 243,210 + -1.0 243,211 - -0.4 243,211 - +0.0 243,487 + GFF3748 0.26 -0.1 243,487 + GFF3748 0.26 +0.2 243,487 + GFF3748 0.26 -0.4 243,487 + GFF3748 0.26 +1.0 243,487 + GFF3748 0.26 +0.6 243,487 + GFF3748 0.26 +0.8 243,487 + GFF3748 0.26 -1.1 243,488 - GFF3748 0.26 -1.3 243,488 - GFF3748 0.26 +0.4 243,488 - GFF3748 0.26 -0.1 243,488 - GFF3748 0.26 -0.1 243,488 - GFF3748 0.26 -1.3 243,488 - GFF3748 0.26 -1.1 243,488 - GFF3748 0.26 -2.5 243,622 + GFF3748 0.42 +0.5 243,745 + GFF3748 0.56 -0.8 243,745 + GFF3748 0.56 -0.7 243,746 - GFF3748 0.57 -1.7 243,746 - GFF3748 0.57 -0.7 243,788 - GFF3748 0.61 -0.1 243,788 - GFF3748 0.61 -0.1 243,788 - GFF3748 0.61 -1.0 243,826 + GFF3748 0.66 -0.6 243,826 + GFF3748 0.66 -0.2 243,827 - GFF3748 0.66 +0.5 243,923 - GFF3748 0.77 +0.3 243,923 - GFF3748 0.77 -1.0 244,051 + -0.1 244,051 + +1.9 244,051 + -2.0 244,051 + -0.3 244,051 + +0.7 244,052 - -0.3 244,052 - -1.2 244,052 - -1.0 244,052 - -0.8 244,052 - +0.7 244,052 - -1.0 244,052 - -0.4 244,143 - -0.1 244,228 + -1.3 244,229 - -0.7 244,558 + GFF3749 0.45 -1.1 245,070 + GFF3750 0.19 -0.6 245,071 - GFF3750 0.19 -0.2 245,071 - GFF3750 0.19 -1.1 245,071 - GFF3750 0.19 -0.9 245,073 + GFF3750 0.20 +1.9 245,073 + GFF3750 0.20 -0.9 245,073 + GFF3750 0.20 +1.1 245,074 - GFF3750 0.20 -1.9 245,232 + GFF3750 0.59 -3.2 245,232 + GFF3750 0.59 -0.4 245,233 - GFF3750 0.59 -1.3 245,233 - GFF3750 0.59 -0.2 245,233 - GFF3750 0.59 -0.3 245,233 - GFF3750 0.59 -0.8 245,233 - GFF3750 0.59 +0.4 245,233 - GFF3750 0.59 +1.3 245,271 + GFF3750 0.69 +0.7 245,271 + GFF3750 0.69 +0.1 245,271 + GFF3750 0.69 -0.4 245,271 + GFF3750 0.69 +1.1 245,271 + GFF3750 0.69 -0.4 245,272 - GFF3750 0.69 -2.0 245,272 - GFF3750 0.69 +0.4 245,272 - GFF3750 0.69 +0.9 245,272 - GFF3750 0.69 +0.3 245,272 - GFF3750 0.69 -1.5 245,272 - GFF3750 0.69 +0.9 245,334 + GFF3750 0.85 -1.1 245,334 + GFF3750 0.85 -0.1 245,334 + GFF3750 0.85 -0.4 245,335 - GFF3750 0.85 -0.5 245,335 - GFF3750 0.85 +0.5 245,335 - GFF3750 0.85 -1.0 245,384 + -0.3 245,384 + -0.1 245,385 - -0.5 245,385 - -0.1 245,385 - -0.2 245,385 - +0.3 245,419 + -0.7 245,419 + +0.2 245,420 - +0.5 245,491 + -1.8 245,491 + -0.5 245,491 + -0.9 245,492 - +0.1 245,492 - -0.3 245,492 - +0.8 245,492 - -0.4 245,492 - +1.6 245,539 + GFF3751 0.13 +0.4 245,539 + GFF3751 0.13 -0.5 245,540 - GFF3751 0.13 +0.1 245,689 + GFF3751 0.32 -0.6 245,689 + GFF3751 0.32 -0.1 245,689 + GFF3751 0.32 -0.1 245,689 + GFF3751 0.32 +0.4 245,690 - GFF3751 0.32 -0.3 245,690 - GFF3751 0.32 +1.5 245,690 - GFF3751 0.32 +0.1 245,690 - GFF3751 0.32 -0.6 245,690 - GFF3751 0.32 -0.5 245,690 - GFF3751 0.32 -0.6 245,914 + GFF3751 0.61 +2.0
Or see this region's nucleotide sequence