Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3719 and GFF3720 are separated by 217 nucleotides GFF3720 and GFF3721 are separated by 66 nucleotides GFF3721 and GFF3722 are separated by 19 nucleotides GFF3722 and GFF3723 are separated by 48 nucleotides
GFF3719 - Pyrimidine monooxygenase RutA (EC 1.14.99.46), at 216,176 to 217,288
GFF3719
GFF3720 - Alternative dihydrofolate reductase 3, at 217,506 to 217,958
GFF3720
GFF3721 - Hydrolase, alpha/beta fold family, at 218,025 to 219,002
GFF3721
GFF3722 - FIG00931152: hypothetical protein, at 219,022 to 219,582
GFF3722
GFF3723 - Cytochrome c5, at 219,631 to 220,320
GFF3723
Position (kb) 218
219
220 Strain fitness (log2 ratio) -2
-1
0
1
2 at 217.221 kb on + strand at 217.222 kb on - strand at 217.222 kb on - strand at 217.222 kb on - strand at 217.222 kb on - strand at 217.222 kb on - strand at 217.346 kb on - strand at 217.355 kb on + strand at 217.368 kb on + strand at 217.369 kb on - strand at 217.369 kb on - strand at 217.372 kb on - strand at 217.575 kb on + strand, within GFF3720 at 217.575 kb on + strand, within GFF3720 at 217.575 kb on + strand, within GFF3720 at 217.575 kb on + strand, within GFF3720 at 217.576 kb on - strand, within GFF3720 at 217.576 kb on - strand, within GFF3720 at 217.648 kb on + strand, within GFF3720 at 217.648 kb on + strand, within GFF3720 at 217.648 kb on + strand, within GFF3720 at 217.648 kb on + strand, within GFF3720 at 217.649 kb on - strand, within GFF3720 at 217.854 kb on + strand, within GFF3720 at 217.854 kb on + strand, within GFF3720 at 217.855 kb on - strand, within GFF3720 at 217.855 kb on - strand, within GFF3720 at 217.855 kb on - strand, within GFF3720 at 217.855 kb on - strand, within GFF3720 at 217.855 kb on - strand, within GFF3720 at 218.031 kb on + strand at 218.088 kb on + strand at 218.088 kb on + strand at 218.088 kb on + strand at 218.089 kb on - strand at 218.247 kb on + strand, within GFF3721 at 218.247 kb on + strand, within GFF3721 at 218.247 kb on + strand, within GFF3721 at 218.247 kb on + strand, within GFF3721 at 218.248 kb on - strand, within GFF3721 at 218.349 kb on + strand, within GFF3721 at 218.350 kb on - strand, within GFF3721 at 218.350 kb on - strand, within GFF3721 at 218.530 kb on + strand, within GFF3721 at 218.595 kb on + strand, within GFF3721 at 218.700 kb on + strand, within GFF3721 at 218.700 kb on + strand, within GFF3721 at 218.700 kb on + strand, within GFF3721 at 218.700 kb on + strand, within GFF3721 at 218.700 kb on + strand, within GFF3721 at 218.701 kb on - strand, within GFF3721 at 218.701 kb on - strand, within GFF3721 at 218.757 kb on + strand, within GFF3721 at 218.757 kb on + strand, within GFF3721 at 218.757 kb on + strand, within GFF3721 at 218.758 kb on - strand, within GFF3721 at 218.758 kb on - strand, within GFF3721 at 218.758 kb on - strand, within GFF3721 at 219.015 kb on + strand at 219.015 kb on + strand at 219.016 kb on - strand at 219.016 kb on - strand at 219.077 kb on - strand at 219.077 kb on - strand at 219.077 kb on - strand at 219.112 kb on + strand, within GFF3722 at 219.112 kb on + strand, within GFF3722 at 219.112 kb on + strand, within GFF3722 at 219.112 kb on + strand, within GFF3722 at 219.112 kb on + strand, within GFF3722 at 219.113 kb on - strand, within GFF3722 at 219.113 kb on - strand, within GFF3722 at 219.113 kb on - strand, within GFF3722 at 219.113 kb on - strand, within GFF3722 at 219.113 kb on - strand, within GFF3722 at 219.113 kb on - strand, within GFF3722 at 219.113 kb on - strand, within GFF3722 at 219.113 kb on - strand, within GFF3722 at 219.211 kb on + strand, within GFF3722 at 219.211 kb on + strand, within GFF3722 at 219.212 kb on - strand, within GFF3722 at 219.212 kb on - strand, within GFF3722 at 219.265 kb on + strand, within GFF3722 at 219.265 kb on + strand, within GFF3722 at 219.266 kb on - strand, within GFF3722 at 219.266 kb on - strand, within GFF3722 at 219.266 kb on - strand, within GFF3722 at 219.266 kb on - strand, within GFF3722 at 219.266 kb on - strand, within GFF3722 at 219.397 kb on + strand, within GFF3722 at 219.397 kb on + strand, within GFF3722 at 219.397 kb on + strand, within GFF3722 at 219.398 kb on - strand, within GFF3722 at 219.398 kb on - strand, within GFF3722 at 219.398 kb on - strand, within GFF3722 at 219.398 kb on - strand, within GFF3722 at 219.620 kb on - strand at 219.620 kb on - strand at 219.624 kb on + strand at 219.625 kb on - strand at 219.632 kb on + strand at 219.632 kb on + strand at 219.633 kb on - strand at 219.633 kb on - strand at 219.821 kb on + strand, within GFF3723 at 219.821 kb on + strand, within GFF3723 at 219.821 kb on + strand, within GFF3723 at 219.821 kb on + strand, within GFF3723 at 219.821 kb on + strand, within GFF3723 at 219.821 kb on + strand, within GFF3723 at 219.821 kb on + strand, within GFF3723 at 219.821 kb on + strand, within GFF3723 at 219.822 kb on - strand, within GFF3723 at 219.822 kb on - strand, within GFF3723 at 219.822 kb on - strand, within GFF3723 at 219.822 kb on - strand, within GFF3723 at 219.822 kb on - strand, within GFF3723 at 219.921 kb on - strand, within GFF3723 at 219.921 kb on - strand, within GFF3723 at 219.921 kb on - strand, within GFF3723
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 217,221 + -0.5 217,222 - -1.0 217,222 - -0.1 217,222 - -0.6 217,222 - +0.1 217,222 - -0.3 217,346 - -1.5 217,355 + +0.4 217,368 + -1.2 217,369 - +0.8 217,369 - -0.6 217,372 - +0.6 217,575 + GFF3720 0.15 -0.3 217,575 + GFF3720 0.15 -0.9 217,575 + GFF3720 0.15 -0.9 217,575 + GFF3720 0.15 +0.2 217,576 - GFF3720 0.15 +0.0 217,576 - GFF3720 0.15 +1.1 217,648 + GFF3720 0.31 -0.8 217,648 + GFF3720 0.31 +0.3 217,648 + GFF3720 0.31 -0.4 217,648 + GFF3720 0.31 +1.3 217,649 - GFF3720 0.32 -0.1 217,854 + GFF3720 0.77 +0.1 217,854 + GFF3720 0.77 -0.1 217,855 - GFF3720 0.77 -1.8 217,855 - GFF3720 0.77 +1.1 217,855 - GFF3720 0.77 -1.4 217,855 - GFF3720 0.77 -0.2 217,855 - GFF3720 0.77 +0.5 218,031 + -0.8 218,088 + -0.8 218,088 + -2.1 218,088 + -1.3 218,089 - -0.1 218,247 + GFF3721 0.23 +0.0 218,247 + GFF3721 0.23 +0.9 218,247 + GFF3721 0.23 -0.4 218,247 + GFF3721 0.23 +0.6 218,248 - GFF3721 0.23 +0.4 218,349 + GFF3721 0.33 +0.1 218,350 - GFF3721 0.33 -1.7 218,350 - GFF3721 0.33 +0.1 218,530 + GFF3721 0.52 -0.9 218,595 + GFF3721 0.58 -0.0 218,700 + GFF3721 0.69 -1.1 218,700 + GFF3721 0.69 +0.1 218,700 + GFF3721 0.69 +0.6 218,700 + GFF3721 0.69 -1.1 218,700 + GFF3721 0.69 -0.5 218,701 - GFF3721 0.69 -0.6 218,701 - GFF3721 0.69 -1.2 218,757 + GFF3721 0.75 +0.3 218,757 + GFF3721 0.75 +0.2 218,757 + GFF3721 0.75 -0.9 218,758 - GFF3721 0.75 +1.9 218,758 - GFF3721 0.75 +1.3 218,758 - GFF3721 0.75 +1.4 219,015 + -0.9 219,015 + -0.6 219,016 - +1.6 219,016 - -0.8 219,077 - -0.9 219,077 - -0.1 219,077 - -1.0 219,112 + GFF3722 0.16 -0.3 219,112 + GFF3722 0.16 -0.1 219,112 + GFF3722 0.16 -0.1 219,112 + GFF3722 0.16 -0.8 219,112 + GFF3722 0.16 -0.6 219,113 - GFF3722 0.16 -1.1 219,113 - GFF3722 0.16 +0.9 219,113 - GFF3722 0.16 -0.1 219,113 - GFF3722 0.16 +0.4 219,113 - GFF3722 0.16 -0.1 219,113 - GFF3722 0.16 +0.0 219,113 - GFF3722 0.16 -0.1 219,113 - GFF3722 0.16 +0.0 219,211 + GFF3722 0.34 -0.8 219,211 + GFF3722 0.34 -0.3 219,212 - GFF3722 0.34 +1.0 219,212 - GFF3722 0.34 +0.9 219,265 + GFF3722 0.43 +0.1 219,265 + GFF3722 0.43 -0.9 219,266 - GFF3722 0.43 +0.5 219,266 - GFF3722 0.43 -0.6 219,266 - GFF3722 0.43 -1.5 219,266 - GFF3722 0.43 -0.2 219,266 - GFF3722 0.43 +0.3 219,397 + GFF3722 0.67 -0.7 219,397 + GFF3722 0.67 +0.7 219,397 + GFF3722 0.67 +0.6 219,398 - GFF3722 0.67 -0.4 219,398 - GFF3722 0.67 -0.2 219,398 - GFF3722 0.67 -0.5 219,398 - GFF3722 0.67 -0.3 219,620 - -0.2 219,620 - -0.1 219,624 + +0.7 219,625 - +0.4 219,632 + +0.2 219,632 + -0.4 219,633 - +0.2 219,633 - -1.5 219,821 + GFF3723 0.28 -1.2 219,821 + GFF3723 0.28 -2.4 219,821 + GFF3723 0.28 +0.8 219,821 + GFF3723 0.28 +0.0 219,821 + GFF3723 0.28 -1.5 219,821 + GFF3723 0.28 -1.7 219,821 + GFF3723 0.28 -0.9 219,821 + GFF3723 0.28 -0.1 219,822 - GFF3723 0.28 -1.0 219,822 - GFF3723 0.28 -1.3 219,822 - GFF3723 0.28 -2.1 219,822 - GFF3723 0.28 -0.9 219,822 - GFF3723 0.28 -1.2 219,921 - GFF3723 0.42 -1.7 219,921 - GFF3723 0.42 -1.4 219,921 - GFF3723 0.42 +0.9
Or see this region's nucleotide sequence