Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3533 and GFF3534 are separated by 161 nucleotides GFF3534 and GFF3535 are separated by 4 nucleotides GFF3535 and GFF3536 are separated by 186 nucleotides
GFF3533 - Two-component system sensor histidine kinase, at 39,285 to 40,685
GFF3533
GFF3534 - hypothetical protein, at 40,847 to 41,185
GFF3534
GFF3535 - Aquaporin Z, at 41,190 to 41,897
GFF3535
GFF3536 - Alcohol dehydrogenase (EC 1.1.1.1), at 42,084 to 43,094
GFF3536
Position (kb)
41
42 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 40.383 kb on + strand, within GFF3533 at 40.383 kb on + strand, within GFF3533 at 40.383 kb on + strand, within GFF3533 at 40.383 kb on + strand, within GFF3533 at 40.384 kb on - strand, within GFF3533 at 40.639 kb on + strand at 40.639 kb on + strand at 40.640 kb on - strand at 40.640 kb on - strand at 40.640 kb on - strand at 40.680 kb on + strand at 40.681 kb on - strand at 40.706 kb on + strand at 40.706 kb on + strand at 40.706 kb on + strand at 40.706 kb on + strand at 40.730 kb on + strand at 40.730 kb on + strand at 40.730 kb on + strand at 40.730 kb on + strand at 40.766 kb on + strand at 40.766 kb on + strand at 40.767 kb on - strand at 40.767 kb on - strand at 40.852 kb on + strand at 40.852 kb on + strand at 40.852 kb on + strand at 40.852 kb on + strand at 40.852 kb on + strand at 40.853 kb on - strand at 40.853 kb on - strand at 40.853 kb on - strand at 40.853 kb on - strand at 40.853 kb on - strand at 40.853 kb on - strand at 40.853 kb on - strand at 40.853 kb on - strand at 41.102 kb on + strand, within GFF3534 at 41.225 kb on - strand at 41.293 kb on + strand, within GFF3535 at 41.294 kb on - strand, within GFF3535 at 41.294 kb on - strand, within GFF3535 at 41.294 kb on - strand, within GFF3535 at 41.294 kb on - strand, within GFF3535 at 41.475 kb on + strand, within GFF3535 at 41.475 kb on + strand, within GFF3535 at 41.475 kb on + strand, within GFF3535 at 41.475 kb on + strand, within GFF3535 at 41.475 kb on + strand, within GFF3535 at 41.475 kb on + strand, within GFF3535 at 41.476 kb on - strand, within GFF3535 at 41.476 kb on - strand, within GFF3535 at 41.506 kb on + strand, within GFF3535 at 41.506 kb on + strand, within GFF3535 at 41.506 kb on + strand, within GFF3535 at 41.506 kb on + strand, within GFF3535 at 41.506 kb on + strand, within GFF3535 at 41.506 kb on + strand, within GFF3535 at 41.506 kb on + strand, within GFF3535 at 41.507 kb on - strand, within GFF3535 at 41.507 kb on - strand, within GFF3535 at 41.507 kb on - strand, within GFF3535 at 41.507 kb on - strand, within GFF3535 at 41.507 kb on - strand, within GFF3535 at 41.587 kb on + strand, within GFF3535 at 41.587 kb on + strand, within GFF3535 at 41.587 kb on + strand, within GFF3535 at 41.588 kb on - strand, within GFF3535 at 41.588 kb on - strand, within GFF3535 at 41.588 kb on - strand, within GFF3535 at 41.635 kb on + strand, within GFF3535 at 41.636 kb on - strand, within GFF3535 at 41.731 kb on + strand, within GFF3535 at 41.731 kb on + strand, within GFF3535 at 41.731 kb on + strand, within GFF3535 at 41.852 kb on + strand at 41.959 kb on + strand at 41.959 kb on + strand at 41.959 kb on + strand at 41.960 kb on - strand at 41.960 kb on - strand at 41.960 kb on - strand at 42.160 kb on + strand at 42.161 kb on - strand at 42.161 kb on - strand at 42.280 kb on + strand, within GFF3536 at 42.280 kb on + strand, within GFF3536 at 42.280 kb on + strand, within GFF3536 at 42.280 kb on + strand, within GFF3536 at 42.280 kb on + strand, within GFF3536 at 42.280 kb on + strand, within GFF3536 at 42.281 kb on - strand, within GFF3536 at 42.281 kb on - strand, within GFF3536 at 42.281 kb on - strand, within GFF3536 at 42.281 kb on - strand, within GFF3536 at 42.281 kb on - strand, within GFF3536 at 42.281 kb on - strand, within GFF3536 at 42.281 kb on - strand, within GFF3536
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 40,383 + GFF3533 0.78 -1.9 40,383 + GFF3533 0.78 +0.7 40,383 + GFF3533 0.78 +0.2 40,383 + GFF3533 0.78 -0.1 40,384 - GFF3533 0.78 +0.6 40,639 + -1.2 40,639 + +0.2 40,640 - -0.8 40,640 - +0.9 40,640 - -0.1 40,680 + -0.8 40,681 - +0.6 40,706 + -2.2 40,706 + +0.1 40,706 + +1.3 40,706 + -2.1 40,730 + -1.2 40,730 + -0.2 40,730 + -0.7 40,730 + -0.1 40,766 + +0.9 40,766 + -0.3 40,767 - -1.4 40,767 - -0.5 40,852 + +0.0 40,852 + -0.2 40,852 + -0.5 40,852 + -0.6 40,852 + -0.3 40,853 - -0.0 40,853 - -1.0 40,853 - -0.3 40,853 - +0.0 40,853 - -1.1 40,853 - -0.4 40,853 - -0.5 40,853 - -0.1 41,102 + GFF3534 0.75 -0.3 41,225 - -0.4 41,293 + GFF3535 0.15 +0.3 41,294 - GFF3535 0.15 -0.9 41,294 - GFF3535 0.15 +2.6 41,294 - GFF3535 0.15 +0.0 41,294 - GFF3535 0.15 -2.1 41,475 + GFF3535 0.40 +1.1 41,475 + GFF3535 0.40 -0.0 41,475 + GFF3535 0.40 +0.5 41,475 + GFF3535 0.40 +0.9 41,475 + GFF3535 0.40 +0.1 41,475 + GFF3535 0.40 -1.4 41,476 - GFF3535 0.40 -1.8 41,476 - GFF3535 0.40 +0.0 41,506 + GFF3535 0.45 -0.2 41,506 + GFF3535 0.45 -0.6 41,506 + GFF3535 0.45 +1.0 41,506 + GFF3535 0.45 -1.1 41,506 + GFF3535 0.45 +0.3 41,506 + GFF3535 0.45 +0.0 41,506 + GFF3535 0.45 -2.6 41,507 - GFF3535 0.45 -0.8 41,507 - GFF3535 0.45 -0.9 41,507 - GFF3535 0.45 -0.7 41,507 - GFF3535 0.45 -0.3 41,507 - GFF3535 0.45 -0.4 41,587 + GFF3535 0.56 +0.4 41,587 + GFF3535 0.56 +0.6 41,587 + GFF3535 0.56 -0.4 41,588 - GFF3535 0.56 -0.1 41,588 - GFF3535 0.56 +0.5 41,588 - GFF3535 0.56 -0.1 41,635 + GFF3535 0.63 +0.4 41,636 - GFF3535 0.63 -1.4 41,731 + GFF3535 0.76 -0.1 41,731 + GFF3535 0.76 +0.4 41,731 + GFF3535 0.76 +0.1 41,852 + +0.9 41,959 + -0.7 41,959 + +0.6 41,959 + -0.1 41,960 - -0.2 41,960 - -0.9 41,960 - -0.5 42,160 + -0.1 42,161 - +0.5 42,161 - -1.3 42,280 + GFF3536 0.19 -0.7 42,280 + GFF3536 0.19 -1.3 42,280 + GFF3536 0.19 +0.3 42,280 + GFF3536 0.19 -0.7 42,280 + GFF3536 0.19 -0.9 42,280 + GFF3536 0.19 -2.1 42,281 - GFF3536 0.19 +2.2 42,281 - GFF3536 0.19 +0.2 42,281 - GFF3536 0.19 -0.7 42,281 - GFF3536 0.19 -0.1 42,281 - GFF3536 0.19 -0.4 42,281 - GFF3536 0.19 -0.1 42,281 - GFF3536 0.19 -0.2
Or see this region's nucleotide sequence