Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3460 and GFF3461 are separated by 18 nucleotides GFF3461 and GFF3462 overlap by 4 nucleotides GFF3462 and GFF3463 are separated by 4 nucleotides GFF3463 and GFF3464 are separated by 5 nucleotides
GFF3460 - Urea ABC transporter, ATPase protein UrtE, at 282,286 to 282,975
GFF3460
GFF3461 - Urea ABC transporter, ATPase protein UrtD, at 282,994 to 283,794
GFF3461
GFF3462 - Urea ABC transporter, permease protein UrtC, at 283,791 to 284,846
GFF3462
GFF3463 - Urea ABC transporter, permease protein UrtB, at 284,851 to 285,753
GFF3463
GFF3464 - Urea ABC transporter, substrate-binding protein UrtA, at 285,759 to 286,952
GFF3464
Position (kb) 283
284
285 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 283.007 kb on + strand at 283.008 kb on - strand at 283.897 kb on + strand, within GFF3462 at 283.930 kb on + strand, within GFF3462 at 284.006 kb on - strand, within GFF3462 at 284.074 kb on + strand, within GFF3462 at 284.074 kb on + strand, within GFF3462 at 284.075 kb on - strand, within GFF3462 at 284.146 kb on + strand, within GFF3462 at 284.147 kb on - strand, within GFF3462 at 284.269 kb on + strand, within GFF3462 at 284.419 kb on + strand, within GFF3462 at 284.420 kb on - strand, within GFF3462 at 284.452 kb on + strand, within GFF3462 at 284.464 kb on + strand, within GFF3462 at 284.465 kb on - strand, within GFF3462 at 284.465 kb on - strand, within GFF3462 at 284.465 kb on - strand, within GFF3462 at 284.465 kb on - strand, within GFF3462 at 284.723 kb on - strand, within GFF3462 at 284.723 kb on - strand, within GFF3462 at 284.723 kb on - strand, within GFF3462 at 284.894 kb on + strand at 284.894 kb on + strand at 284.895 kb on - strand at 284.895 kb on - strand at 284.895 kb on - strand at 284.954 kb on + strand, within GFF3463 at 284.954 kb on + strand, within GFF3463 at 284.955 kb on - strand, within GFF3463 at 285.059 kb on + strand, within GFF3463 at 285.060 kb on - strand, within GFF3463 at 285.060 kb on - strand, within GFF3463 at 285.060 kb on - strand, within GFF3463 at 285.060 kb on - strand, within GFF3463 at 285.060 kb on - strand, within GFF3463 at 285.060 kb on - strand, within GFF3463 at 285.254 kb on + strand, within GFF3463 at 285.398 kb on + strand, within GFF3463 at 285.461 kb on + strand, within GFF3463 at 285.495 kb on - strand, within GFF3463 at 285.545 kb on + strand, within GFF3463 at 285.546 kb on - strand, within GFF3463 at 285.546 kb on - strand, within GFF3463 at 285.596 kb on + strand, within GFF3463 at 285.596 kb on + strand, within GFF3463 at 285.597 kb on - strand, within GFF3463 at 285.605 kb on + strand, within GFF3463 at 285.606 kb on - strand, within GFF3463
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 283,007 + -0.2 283,008 - -0.4 283,897 + GFF3462 0.10 +0.4 283,930 + GFF3462 0.13 +0.8 284,006 - GFF3462 0.20 +1.6 284,074 + GFF3462 0.27 -0.9 284,074 + GFF3462 0.27 +0.7 284,075 - GFF3462 0.27 -0.8 284,146 + GFF3462 0.34 +2.5 284,147 - GFF3462 0.34 -0.4 284,269 + GFF3462 0.45 -0.1 284,419 + GFF3462 0.59 -1.4 284,420 - GFF3462 0.60 -0.8 284,452 + GFF3462 0.63 -0.3 284,464 + GFF3462 0.64 -2.1 284,465 - GFF3462 0.64 -1.1 284,465 - GFF3462 0.64 -0.1 284,465 - GFF3462 0.64 -0.1 284,465 - GFF3462 0.64 -0.2 284,723 - GFF3462 0.88 -1.7 284,723 - GFF3462 0.88 -2.5 284,723 - GFF3462 0.88 -1.8 284,894 + -0.7 284,894 + +0.3 284,895 - -0.1 284,895 - -0.4 284,895 - -0.1 284,954 + GFF3463 0.11 -0.1 284,954 + GFF3463 0.11 -0.3 284,955 - GFF3463 0.12 -0.3 285,059 + GFF3463 0.23 -0.1 285,060 - GFF3463 0.23 -0.4 285,060 - GFF3463 0.23 -0.1 285,060 - GFF3463 0.23 -0.3 285,060 - GFF3463 0.23 -1.1 285,060 - GFF3463 0.23 +0.1 285,060 - GFF3463 0.23 +0.2 285,254 + GFF3463 0.45 -0.2 285,398 + GFF3463 0.61 -3.0 285,461 + GFF3463 0.68 -0.3 285,495 - GFF3463 0.71 -1.3 285,545 + GFF3463 0.77 -0.9 285,546 - GFF3463 0.77 -0.2 285,546 - GFF3463 0.77 +0.2 285,596 + GFF3463 0.83 -0.9 285,596 + GFF3463 0.83 +0.2 285,597 - GFF3463 0.83 -0.4 285,605 + GFF3463 0.83 +1.7 285,606 - GFF3463 0.84 -0.1
Or see this region's nucleotide sequence