Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3457 and GFF3458 overlap by 4 nucleotides GFF3458 and GFF3459 overlap by 4 nucleotides GFF3459 and GFF3460 are separated by 39 nucleotides GFF3460 and GFF3461 are separated by 18 nucleotides
GFF3457 - Nitric oxide reductase activation protein NorD, at 278,745 to 280,397
GFF3457
GFF3458 - Rubisco activation protein CbbQ, at 280,394 to 281,212
GFF3458
GFF3459 - Aliphatic amidase AmiE (EC 3.5.1.4), at 281,209 to 282,246
GFF3459
GFF3460 - Urea ABC transporter, ATPase protein UrtE, at 282,286 to 282,975
GFF3460
GFF3461 - Urea ABC transporter, ATPase protein UrtD, at 282,994 to 283,794
GFF3461
Position (kb) 281
282
283 Strain fitness (log2 ratio) -2
-1
0
1
2 at 280.525 kb on - strand, within GFF3458 at 280.752 kb on + strand, within GFF3458 at 280.753 kb on - strand, within GFF3458 at 280.817 kb on + strand, within GFF3458 at 280.817 kb on + strand, within GFF3458 at 280.817 kb on + strand, within GFF3458 at 280.890 kb on + strand, within GFF3458 at 281.483 kb on + strand, within GFF3459 at 281.562 kb on - strand, within GFF3459 at 281.562 kb on - strand, within GFF3459 at 281.607 kb on - strand, within GFF3459 at 281.666 kb on + strand, within GFF3459 at 281.673 kb on - strand, within GFF3459 at 281.673 kb on - strand, within GFF3459 at 281.795 kb on + strand, within GFF3459 at 281.796 kb on - strand, within GFF3459 at 281.796 kb on - strand, within GFF3459 at 281.900 kb on + strand, within GFF3459 at 282.029 kb on + strand, within GFF3459 at 282.029 kb on + strand, within GFF3459 at 282.029 kb on + strand, within GFF3459 at 282.030 kb on - strand, within GFF3459 at 282.047 kb on + strand, within GFF3459 at 282.047 kb on + strand, within GFF3459 at 282.047 kb on + strand, within GFF3459 at 282.047 kb on + strand, within GFF3459 at 282.048 kb on - strand, within GFF3459 at 282.287 kb on + strand at 282.287 kb on + strand at 282.288 kb on - strand at 282.765 kb on - strand, within GFF3460 at 283.007 kb on + strand at 283.008 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 280,525 - GFF3458 0.16 +0.9 280,752 + GFF3458 0.44 +0.6 280,753 - GFF3458 0.44 +0.1 280,817 + GFF3458 0.52 -2.6 280,817 + GFF3458 0.52 -0.1 280,817 + GFF3458 0.52 +0.2 280,890 + GFF3458 0.61 -0.3 281,483 + GFF3459 0.26 -1.7 281,562 - GFF3459 0.34 -0.4 281,562 - GFF3459 0.34 -0.4 281,607 - GFF3459 0.38 -1.9 281,666 + GFF3459 0.44 -1.2 281,673 - GFF3459 0.45 -0.5 281,673 - GFF3459 0.45 +0.1 281,795 + GFF3459 0.56 +0.9 281,796 - GFF3459 0.57 -0.9 281,796 - GFF3459 0.57 -0.7 281,900 + GFF3459 0.67 -0.5 282,029 + GFF3459 0.79 -0.5 282,029 + GFF3459 0.79 -0.3 282,029 + GFF3459 0.79 +0.8 282,030 - GFF3459 0.79 +1.9 282,047 + GFF3459 0.81 -1.9 282,047 + GFF3459 0.81 +0.4 282,047 + GFF3459 0.81 +0.9 282,047 + GFF3459 0.81 -0.3 282,048 - GFF3459 0.81 -0.6 282,287 + +0.3 282,287 + -0.2 282,288 - +0.9 282,765 - GFF3460 0.69 -0.5 283,007 + -0.2 283,008 - -0.4
Or see this region's nucleotide sequence