Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3417 and GFF3418 overlap by 8 nucleotides GFF3418 and GFF3419 are separated by 74 nucleotides GFF3419 and GFF3420 overlap by 4 nucleotides GFF3420 and GFF3421 are separated by 100 nucleotides
GFF3417 - Alkanesulfonates transport system permease protein, at 240,959 to 241,729
GFF3417
GFF3418 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 241,722 to 242,501
GFF3418
GFF3419 - ABC transporter, substrate-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 242,576 to 243,535
GFF3419
GFF3420 - Transcriptional regulator, AcrR family, at 243,532 to 244,284
GFF3420
GFF3421 - Acetoacetyl-CoA synthetase (EC 6.2.1.16), at 244,385 to 246,388
GFF3421
Position (kb) 242
243
244 Strain fitness (log2 ratio) -2
-1
0
1
2 at 241.653 kb on + strand at 241.654 kb on - strand at 241.670 kb on + strand at 241.775 kb on - strand at 241.900 kb on + strand, within GFF3418 at 241.900 kb on + strand, within GFF3418 at 241.901 kb on - strand, within GFF3418 at 241.901 kb on - strand, within GFF3418 at 241.940 kb on + strand, within GFF3418 at 242.334 kb on + strand, within GFF3418 at 242.334 kb on + strand, within GFF3418 at 242.730 kb on + strand, within GFF3419 at 242.730 kb on + strand, within GFF3419 at 242.730 kb on + strand, within GFF3419 at 242.730 kb on + strand, within GFF3419 at 242.730 kb on + strand, within GFF3419 at 242.731 kb on - strand, within GFF3419 at 242.731 kb on - strand, within GFF3419 at 242.731 kb on - strand, within GFF3419 at 242.905 kb on - strand, within GFF3419 at 243.042 kb on + strand, within GFF3419 at 243.117 kb on + strand, within GFF3419 at 243.369 kb on + strand, within GFF3419 at 243.369 kb on + strand, within GFF3419 at 243.370 kb on - strand, within GFF3419 at 243.370 kb on - strand, within GFF3419 at 243.797 kb on + strand, within GFF3420 at 243.798 kb on - strand, within GFF3420 at 243.798 kb on - strand, within GFF3420 at 243.798 kb on - strand, within GFF3420 at 243.821 kb on + strand, within GFF3420 at 243.860 kb on + strand, within GFF3420 at 243.860 kb on + strand, within GFF3420 at 243.860 kb on + strand, within GFF3420 at 243.887 kb on + strand, within GFF3420 at 243.888 kb on - strand, within GFF3420 at 243.891 kb on - strand, within GFF3420 at 244.070 kb on + strand, within GFF3420 at 244.070 kb on + strand, within GFF3420 at 244.071 kb on - strand, within GFF3420 at 244.073 kb on + strand, within GFF3420 at 244.073 kb on + strand, within GFF3420 at 244.073 kb on + strand, within GFF3420 at 244.073 kb on + strand, within GFF3420 at 244.073 kb on + strand, within GFF3420 at 244.073 kb on + strand, within GFF3420 at 244.073 kb on + strand, within GFF3420 at 244.074 kb on - strand, within GFF3420 at 244.074 kb on - strand, within GFF3420 at 244.074 kb on - strand, within GFF3420 at 244.074 kb on - strand, within GFF3420 at 244.074 kb on - strand, within GFF3420 at 244.074 kb on - strand, within GFF3420 at 244.074 kb on - strand, within GFF3420 at 244.074 kb on - strand, within GFF3420 at 244.076 kb on + strand, within GFF3420 at 244.077 kb on - strand, within GFF3420 at 244.373 kb on + strand at 244.374 kb on - strand at 244.384 kb on + strand at 244.384 kb on + strand at 244.384 kb on + strand at 244.384 kb on + strand at 244.418 kb on + strand at 244.419 kb on - strand at 244.463 kb on + strand at 244.463 kb on + strand at 244.464 kb on - strand at 244.464 kb on - strand at 244.464 kb on - strand at 244.464 kb on - strand at 244.526 kb on + strand at 244.526 kb on + strand at 244.526 kb on + strand at 244.526 kb on + strand at 244.527 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 241,653 + -0.8 241,654 - +0.3 241,670 + -0.6 241,775 - -0.2 241,900 + GFF3418 0.23 -1.2 241,900 + GFF3418 0.23 -1.0 241,901 - GFF3418 0.23 +1.9 241,901 - GFF3418 0.23 +0.1 241,940 + GFF3418 0.28 +1.3 242,334 + GFF3418 0.78 -0.2 242,334 + GFF3418 0.78 -0.4 242,730 + GFF3419 0.16 -1.0 242,730 + GFF3419 0.16 +0.9 242,730 + GFF3419 0.16 +1.6 242,730 + GFF3419 0.16 +0.9 242,730 + GFF3419 0.16 -0.4 242,731 - GFF3419 0.16 -1.0 242,731 - GFF3419 0.16 -0.7 242,731 - GFF3419 0.16 -0.9 242,905 - GFF3419 0.34 -1.0 243,042 + GFF3419 0.49 -0.2 243,117 + GFF3419 0.56 -0.7 243,369 + GFF3419 0.83 -0.1 243,369 + GFF3419 0.83 +0.5 243,370 - GFF3419 0.83 -1.5 243,370 - GFF3419 0.83 -0.1 243,797 + GFF3420 0.35 -0.4 243,798 - GFF3420 0.35 +0.5 243,798 - GFF3420 0.35 -0.6 243,798 - GFF3420 0.35 +0.7 243,821 + GFF3420 0.38 -0.7 243,860 + GFF3420 0.44 +0.2 243,860 + GFF3420 0.44 -0.1 243,860 + GFF3420 0.44 +2.2 243,887 + GFF3420 0.47 -0.2 243,888 - GFF3420 0.47 +0.7 243,891 - GFF3420 0.48 +0.1 244,070 + GFF3420 0.71 +0.5 244,070 + GFF3420 0.71 +0.1 244,071 - GFF3420 0.72 +0.6 244,073 + GFF3420 0.72 -0.1 244,073 + GFF3420 0.72 +0.1 244,073 + GFF3420 0.72 +0.5 244,073 + GFF3420 0.72 +0.3 244,073 + GFF3420 0.72 -0.4 244,073 + GFF3420 0.72 -1.2 244,073 + GFF3420 0.72 -0.8 244,074 - GFF3420 0.72 -0.6 244,074 - GFF3420 0.72 -1.1 244,074 - GFF3420 0.72 +0.3 244,074 - GFF3420 0.72 -1.0 244,074 - GFF3420 0.72 -1.0 244,074 - GFF3420 0.72 -1.8 244,074 - GFF3420 0.72 -0.2 244,074 - GFF3420 0.72 +0.3 244,076 + GFF3420 0.72 +1.0 244,077 - GFF3420 0.72 +1.5 244,373 + -0.4 244,374 - -1.0 244,384 + +0.9 244,384 + -0.6 244,384 + +0.1 244,384 + -0.1 244,418 + +0.5 244,419 - +0.3 244,463 + +1.9 244,463 + -0.9 244,464 - -0.8 244,464 - +0.2 244,464 - -0.7 244,464 - +0.5 244,526 + -1.6 244,526 + +1.6 244,526 + -1.1 244,526 + -1.3 244,527 - +0.3
Or see this region's nucleotide sequence