Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3379 and GFF3380 are separated by 126 nucleotides GFF3380 and GFF3381 overlap by 4 nucleotides
GFF3379 - Aromatic--CoA ligase, at 199,083 to 200,894
GFF3379
GFF3380 - 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35), at 201,021 to 201,968
GFF3380
GFF3381 - Broad-specificity amino acid ABC transporter, substrate-binding protein, at 201,965 to 203,173
GFF3381
Position (kb) 201
202 Strain fitness (log2 ratio) -2
-1
0
1
2 at 200.050 kb on + strand, within GFF3379 at 200.050 kb on + strand, within GFF3379 at 200.051 kb on - strand, within GFF3379 at 200.255 kb on - strand, within GFF3379 at 200.420 kb on - strand, within GFF3379 at 200.440 kb on + strand, within GFF3379 at 200.440 kb on + strand, within GFF3379 at 200.440 kb on + strand, within GFF3379 at 200.441 kb on - strand, within GFF3379 at 200.677 kb on + strand, within GFF3379 at 200.677 kb on + strand, within GFF3379 at 200.785 kb on + strand at 200.785 kb on + strand at 200.785 kb on + strand at 200.786 kb on - strand at 200.844 kb on + strand at 200.942 kb on - strand at 201.023 kb on - strand at 201.023 kb on - strand at 201.023 kb on - strand at 201.023 kb on - strand at 201.063 kb on + strand at 201.454 kb on + strand, within GFF3380 at 201.454 kb on + strand, within GFF3380 at 201.686 kb on + strand, within GFF3380 at 202.047 kb on + strand at 202.047 kb on + strand at 202.047 kb on + strand at 202.047 kb on + strand at 202.048 kb on - strand at 202.048 kb on - strand at 202.048 kb on - strand at 202.048 kb on - strand at 202.048 kb on - strand at 202.048 kb on - strand at 202.050 kb on + strand at 202.050 kb on + strand at 202.051 kb on - strand at 202.233 kb on + strand, within GFF3381 at 202.252 kb on - strand, within GFF3381 at 202.342 kb on - strand, within GFF3381 at 202.359 kb on + strand, within GFF3381 at 202.360 kb on - strand, within GFF3381 at 202.671 kb on + strand, within GFF3381 at 202.671 kb on + strand, within GFF3381 at 202.672 kb on - strand, within GFF3381 at 202.737 kb on + strand, within GFF3381 at 202.738 kb on - strand, within GFF3381 at 202.738 kb on - strand, within GFF3381 at 202.743 kb on + strand, within GFF3381 at 202.743 kb on + strand, within GFF3381 at 202.743 kb on + strand, within GFF3381 at 202.755 kb on + strand, within GFF3381 at 202.756 kb on - strand, within GFF3381 at 202.809 kb on + strand, within GFF3381 at 202.967 kb on + strand, within GFF3381 at 202.967 kb on + strand, within GFF3381
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 200,050 + GFF3379 0.53 +0.0 200,050 + GFF3379 0.53 -0.9 200,051 - GFF3379 0.53 -1.7 200,255 - GFF3379 0.65 -0.9 200,420 - GFF3379 0.74 +0.3 200,440 + GFF3379 0.75 -0.9 200,440 + GFF3379 0.75 -1.2 200,440 + GFF3379 0.75 -1.2 200,441 - GFF3379 0.75 +0.8 200,677 + GFF3379 0.88 +0.6 200,677 + GFF3379 0.88 +1.1 200,785 + +0.2 200,785 + -0.3 200,785 + -0.4 200,786 - +0.2 200,844 + -1.0 200,942 - -0.4 201,023 - -1.0 201,023 - -0.7 201,023 - +1.2 201,023 - -2.7 201,063 + -0.3 201,454 + GFF3380 0.46 -0.6 201,454 + GFF3380 0.46 -0.8 201,686 + GFF3380 0.70 -1.5 202,047 + +0.7 202,047 + -0.7 202,047 + -1.0 202,047 + +1.3 202,048 - -0.2 202,048 - +0.3 202,048 - -0.9 202,048 - -1.5 202,048 - +0.5 202,048 - -0.3 202,050 + +0.3 202,050 + +1.9 202,051 - +0.0 202,233 + GFF3381 0.22 -0.6 202,252 - GFF3381 0.24 -0.4 202,342 - GFF3381 0.31 +0.1 202,359 + GFF3381 0.33 +0.3 202,360 - GFF3381 0.33 +1.2 202,671 + GFF3381 0.58 +1.3 202,671 + GFF3381 0.58 -0.4 202,672 - GFF3381 0.58 +0.0 202,737 + GFF3381 0.64 +0.9 202,738 - GFF3381 0.64 -1.9 202,738 - GFF3381 0.64 -0.8 202,743 + GFF3381 0.64 -1.4 202,743 + GFF3381 0.64 -0.8 202,743 + GFF3381 0.64 -0.3 202,755 + GFF3381 0.65 -0.9 202,756 - GFF3381 0.65 -1.1 202,809 + GFF3381 0.70 -0.4 202,967 + GFF3381 0.83 -0.7 202,967 + GFF3381 0.83 +0.0
Or see this region's nucleotide sequence