Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3375 and GFF3376 are separated by 30 nucleotides GFF3376 and GFF3377 are separated by 58 nucleotides GFF3377 and GFF3378 are separated by 79 nucleotides
GFF3375 - DNA-binding transcriptional regulator, MocR family / aminotransferase domain, at 194,368 to 195,756
GFF3375
GFF3376 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 195,787 to 197,343
GFF3376
GFF3377 - Enoyl-CoA hydratase (EC 4.2.1.17) @ Enoyl-CoA hydratase EchA5 (EC 4.2.1.17), at 197,402 to 198,184
GFF3377
GFF3378 - hypothetical protein, at 198,264 to 199,076
GFF3378
Position (kb) 195
196
197
198 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 195.175 kb on + strand, within GFF3375 at 195.176 kb on - strand, within GFF3375 at 195.176 kb on - strand, within GFF3375 at 195.271 kb on + strand, within GFF3375 at 195.272 kb on - strand, within GFF3375 at 195.272 kb on - strand, within GFF3375 at 195.272 kb on - strand, within GFF3375 at 195.272 kb on - strand, within GFF3375 at 195.394 kb on + strand, within GFF3375 at 195.394 kb on + strand, within GFF3375 at 195.395 kb on - strand, within GFF3375 at 195.436 kb on + strand, within GFF3375 at 195.550 kb on + strand, within GFF3375 at 195.550 kb on + strand, within GFF3375 at 195.550 kb on + strand, within GFF3375 at 195.550 kb on + strand, within GFF3375 at 195.550 kb on + strand, within GFF3375 at 195.550 kb on + strand, within GFF3375 at 195.551 kb on - strand, within GFF3375 at 195.884 kb on + strand at 195.884 kb on + strand at 195.885 kb on - strand at 196.061 kb on + strand, within GFF3376 at 196.062 kb on - strand, within GFF3376 at 196.070 kb on + strand, within GFF3376 at 196.071 kb on - strand, within GFF3376 at 196.205 kb on + strand, within GFF3376 at 196.205 kb on + strand, within GFF3376 at 196.205 kb on + strand, within GFF3376 at 196.206 kb on - strand, within GFF3376 at 196.206 kb on - strand, within GFF3376 at 196.208 kb on + strand, within GFF3376 at 196.209 kb on - strand, within GFF3376 at 196.397 kb on + strand, within GFF3376 at 196.397 kb on + strand, within GFF3376 at 196.397 kb on + strand, within GFF3376 at 196.398 kb on - strand, within GFF3376 at 196.398 kb on - strand, within GFF3376 at 196.398 kb on - strand, within GFF3376 at 196.398 kb on - strand, within GFF3376 at 196.400 kb on + strand, within GFF3376 at 196.400 kb on + strand, within GFF3376 at 196.400 kb on + strand, within GFF3376 at 196.401 kb on - strand, within GFF3376 at 196.475 kb on + strand, within GFF3376 at 196.485 kb on - strand, within GFF3376 at 196.660 kb on + strand, within GFF3376 at 196.660 kb on + strand, within GFF3376 at 196.763 kb on + strand, within GFF3376 at 196.763 kb on + strand, within GFF3376 at 196.763 kb on + strand, within GFF3376 at 197.297 kb on + strand at 197.297 kb on + strand at 197.297 kb on + strand at 197.298 kb on - strand at 197.865 kb on + strand, within GFF3377 at 198.172 kb on - strand at 198.172 kb on - strand at 198.172 kb on - strand at 198.238 kb on - strand at 198.256 kb on - strand at 198.274 kb on + strand at 198.274 kb on + strand at 198.274 kb on + strand at 198.274 kb on + strand at 198.275 kb on - strand at 198.275 kb on - strand at 198.275 kb on - strand at 198.275 kb on - strand at 198.278 kb on + strand at 198.278 kb on + strand at 198.278 kb on + strand at 198.279 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 195,175 + GFF3375 0.58 -0.9 195,176 - GFF3375 0.58 +0.4 195,176 - GFF3375 0.58 -0.4 195,271 + GFF3375 0.65 -0.4 195,272 - GFF3375 0.65 -0.7 195,272 - GFF3375 0.65 -0.2 195,272 - GFF3375 0.65 -0.3 195,272 - GFF3375 0.65 -0.0 195,394 + GFF3375 0.74 +0.7 195,394 + GFF3375 0.74 -0.1 195,395 - GFF3375 0.74 -1.1 195,436 + GFF3375 0.77 -0.1 195,550 + GFF3375 0.85 -0.5 195,550 + GFF3375 0.85 +1.3 195,550 + GFF3375 0.85 +0.8 195,550 + GFF3375 0.85 -0.7 195,550 + GFF3375 0.85 -1.1 195,550 + GFF3375 0.85 -0.2 195,551 - GFF3375 0.85 -0.1 195,884 + -2.0 195,884 + -0.7 195,885 - -0.7 196,061 + GFF3376 0.18 +0.9 196,062 - GFF3376 0.18 +0.5 196,070 + GFF3376 0.18 -0.8 196,071 - GFF3376 0.18 -0.9 196,205 + GFF3376 0.27 -1.1 196,205 + GFF3376 0.27 -0.8 196,205 + GFF3376 0.27 -0.4 196,206 - GFF3376 0.27 -1.5 196,206 - GFF3376 0.27 +0.2 196,208 + GFF3376 0.27 -0.4 196,209 - GFF3376 0.27 -0.7 196,397 + GFF3376 0.39 -0.4 196,397 + GFF3376 0.39 -0.7 196,397 + GFF3376 0.39 -0.2 196,398 - GFF3376 0.39 -0.1 196,398 - GFF3376 0.39 +0.3 196,398 - GFF3376 0.39 -0.3 196,398 - GFF3376 0.39 -0.6 196,400 + GFF3376 0.39 +1.5 196,400 + GFF3376 0.39 +4.3 196,400 + GFF3376 0.39 -0.4 196,401 - GFF3376 0.39 -1.1 196,475 + GFF3376 0.44 -1.1 196,485 - GFF3376 0.45 -0.9 196,660 + GFF3376 0.56 -0.9 196,660 + GFF3376 0.56 -0.5 196,763 + GFF3376 0.63 -0.5 196,763 + GFF3376 0.63 +1.3 196,763 + GFF3376 0.63 -0.7 197,297 + -0.6 197,297 + -1.5 197,297 + +1.0 197,298 - -0.7 197,865 + GFF3377 0.59 -1.5 198,172 - -1.1 198,172 - -0.6 198,172 - -1.3 198,238 - -2.0 198,256 - -1.9 198,274 + -1.4 198,274 + +0.2 198,274 + +0.1 198,274 + -1.1 198,275 - -0.2 198,275 - -0.6 198,275 - -0.4 198,275 - -1.1 198,278 + -0.1 198,278 + -0.5 198,278 + -0.5 198,279 - +0.2
Or see this region's nucleotide sequence