Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2976 and GFF2977 overlap by 4 nucleotides GFF2977 and GFF2978 are separated by 7 nucleotides GFF2978 and GFF2979 are separated by 0 nucleotides GFF2979 and GFF2980 are separated by 81 nucleotides
GFF2976 - hypothetical protein, at 407,552 to 408,604
GFF2976
GFF2977 - hypothetical protein, at 408,601 to 409,242
GFF2977
GFF2978 - Aromatic ring-cleaving dioxygenase, at 409,250 to 409,597
GFF2978
GFF2979 - Glutathione S-transferase (EC 2.5.1.18), at 409,598 to 410,254
GFF2979
GFF2980 - Intracellular PHB depolymerase PhaZ2, at 410,336 to 411,556
GFF2980
Position (kb) 409
410 Strain fitness (log2 ratio) -2
-1
0
1 at 408.395 kb on + strand, within GFF2976 at 408.395 kb on + strand, within GFF2976 at 408.395 kb on + strand, within GFF2976 at 408.419 kb on + strand, within GFF2976 at 408.419 kb on + strand, within GFF2976 at 408.419 kb on + strand, within GFF2976 at 408.419 kb on + strand, within GFF2976 at 408.419 kb on + strand, within GFF2976 at 408.419 kb on + strand, within GFF2976 at 408.420 kb on - strand, within GFF2976 at 408.420 kb on - strand, within GFF2976 at 408.420 kb on - strand, within GFF2976 at 408.497 kb on + strand, within GFF2976 at 408.557 kb on + strand at 408.874 kb on + strand, within GFF2977 at 408.970 kb on + strand, within GFF2977 at 408.971 kb on - strand, within GFF2977 at 408.971 kb on - strand, within GFF2977 at 409.046 kb on - strand, within GFF2977 at 409.108 kb on + strand, within GFF2977 at 409.108 kb on + strand, within GFF2977 at 409.108 kb on + strand, within GFF2977 at 409.109 kb on - strand, within GFF2977 at 409.275 kb on - strand at 409.275 kb on - strand at 409.283 kb on + strand at 409.298 kb on + strand, within GFF2978 at 409.298 kb on + strand, within GFF2978 at 409.299 kb on - strand, within GFF2978 at 409.299 kb on - strand, within GFF2978 at 409.299 kb on - strand, within GFF2978 at 409.595 kb on + strand at 409.599 kb on + strand at 409.599 kb on + strand at 409.599 kb on + strand at 409.599 kb on + strand at 409.600 kb on - strand at 409.600 kb on - strand at 409.600 kb on - strand at 409.695 kb on + strand, within GFF2979 at 409.728 kb on + strand, within GFF2979 at 409.729 kb on - strand, within GFF2979 at 409.729 kb on - strand, within GFF2979 at 409.758 kb on + strand, within GFF2979 at 409.758 kb on + strand, within GFF2979 at 409.758 kb on + strand, within GFF2979 at 409.759 kb on - strand, within GFF2979 at 409.759 kb on - strand, within GFF2979 at 409.809 kb on + strand, within GFF2979 at 409.809 kb on + strand, within GFF2979 at 409.810 kb on - strand, within GFF2979 at 409.810 kb on - strand, within GFF2979 at 410.031 kb on + strand, within GFF2979 at 410.217 kb on + strand at 410.217 kb on + strand at 410.217 kb on + strand at 410.218 kb on - strand at 410.218 kb on - strand at 410.351 kb on + strand at 410.399 kb on + strand at 410.399 kb on + strand at 410.399 kb on + strand at 410.399 kb on + strand at 410.400 kb on - strand at 410.400 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 408,395 + GFF2976 0.80 -0.8 408,395 + GFF2976 0.80 +0.0 408,395 + GFF2976 0.80 +0.5 408,419 + GFF2976 0.82 -0.5 408,419 + GFF2976 0.82 +0.2 408,419 + GFF2976 0.82 -0.3 408,419 + GFF2976 0.82 -0.1 408,419 + GFF2976 0.82 -1.6 408,419 + GFF2976 0.82 +0.4 408,420 - GFF2976 0.82 -1.0 408,420 - GFF2976 0.82 +0.1 408,420 - GFF2976 0.82 -0.6 408,497 + GFF2976 0.90 -0.1 408,557 + -0.3 408,874 + GFF2977 0.43 -0.3 408,970 + GFF2977 0.57 +0.4 408,971 - GFF2977 0.58 +0.2 408,971 - GFF2977 0.58 +0.1 409,046 - GFF2977 0.69 -1.3 409,108 + GFF2977 0.79 +0.2 409,108 + GFF2977 0.79 -0.8 409,108 + GFF2977 0.79 -1.2 409,109 - GFF2977 0.79 -0.6 409,275 - +0.4 409,275 - +1.0 409,283 + -1.1 409,298 + GFF2978 0.14 -0.8 409,298 + GFF2978 0.14 -0.8 409,299 - GFF2978 0.14 +1.3 409,299 - GFF2978 0.14 -2.0 409,299 - GFF2978 0.14 -0.1 409,595 + +0.9 409,599 + +0.7 409,599 + +0.1 409,599 + -0.4 409,599 + +0.6 409,600 - +0.2 409,600 - -0.7 409,600 - -0.2 409,695 + GFF2979 0.15 -0.5 409,728 + GFF2979 0.20 -1.3 409,729 - GFF2979 0.20 -0.8 409,729 - GFF2979 0.20 +0.9 409,758 + GFF2979 0.24 -0.1 409,758 + GFF2979 0.24 -0.9 409,758 + GFF2979 0.24 -0.4 409,759 - GFF2979 0.25 -0.0 409,759 - GFF2979 0.25 +0.8 409,809 + GFF2979 0.32 +1.1 409,809 + GFF2979 0.32 -1.0 409,810 - GFF2979 0.32 -1.0 409,810 - GFF2979 0.32 -0.4 410,031 + GFF2979 0.66 +0.5 410,217 + -0.1 410,217 + -0.4 410,217 + +0.1 410,218 - +0.6 410,218 - -0.5 410,351 + -1.0 410,399 + +0.4 410,399 + -0.6 410,399 + +0.7 410,399 + -1.6 410,400 - -1.2 410,400 - +0.3
Or see this region's nucleotide sequence