Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2906 and GFF2907 are separated by 13 nucleotides GFF2907 and GFF2908 are separated by 2 nucleotides
GFF2906 - 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26), at 333,085 to 334,521
GFF2906
GFF2907 - Glucarate dehydratase (EC 4.2.1.40), at 334,535 to 335,893
GFF2907
GFF2908 - BUG/TctC family periplasmic protein, at 335,896 to 336,897
GFF2908
Position (kb) 334
335
336 Strain fitness (log2 ratio) -2
-1
0
1 at 333.642 kb on - strand, within GFF2906 at 333.698 kb on + strand, within GFF2906 at 333.698 kb on + strand, within GFF2906 at 333.699 kb on - strand, within GFF2906 at 333.699 kb on - strand, within GFF2906 at 334.421 kb on + strand at 334.469 kb on + strand at 334.469 kb on + strand at 334.470 kb on - strand at 334.470 kb on - strand at 334.470 kb on - strand at 334.470 kb on - strand at 334.470 kb on - strand at 334.587 kb on + strand at 334.636 kb on - strand at 334.636 kb on - strand at 335.100 kb on + strand, within GFF2907 at 335.101 kb on - strand, within GFF2907 at 335.382 kb on + strand, within GFF2907 at 335.382 kb on + strand, within GFF2907 at 335.415 kb on + strand, within GFF2907 at 335.415 kb on + strand, within GFF2907 at 335.416 kb on - strand, within GFF2907 at 335.438 kb on + strand, within GFF2907 at 335.887 kb on + strand at 335.887 kb on + strand at 335.888 kb on - strand at 336.122 kb on + strand, within GFF2908 at 336.122 kb on + strand, within GFF2908 at 336.123 kb on - strand, within GFF2908 at 336.123 kb on - strand, within GFF2908 at 336.123 kb on - strand, within GFF2908 at 336.317 kb on + strand, within GFF2908 at 336.318 kb on - strand, within GFF2908 at 336.318 kb on - strand, within GFF2908 at 336.318 kb on - strand, within GFF2908 at 336.518 kb on + strand, within GFF2908 at 336.518 kb on + strand, within GFF2908 at 336.518 kb on + strand, within GFF2908 at 336.518 kb on + strand, within GFF2908 at 336.519 kb on - strand, within GFF2908 at 336.519 kb on - strand, within GFF2908 at 336.543 kb on - strand, within GFF2908 at 336.608 kb on + strand, within GFF2908 at 336.609 kb on - strand, within GFF2908 at 336.609 kb on - strand, within GFF2908 at 336.874 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 333,642 - GFF2906 0.39 -0.2 333,698 + GFF2906 0.43 -0.7 333,698 + GFF2906 0.43 -0.7 333,699 - GFF2906 0.43 -1.1 333,699 - GFF2906 0.43 -0.0 334,421 + -0.4 334,469 + +0.2 334,469 + -0.1 334,470 - +0.0 334,470 - +0.1 334,470 - +0.5 334,470 - +0.6 334,470 - +0.1 334,587 + -0.0 334,636 - -1.3 334,636 - -0.3 335,100 + GFF2907 0.42 -0.5 335,101 - GFF2907 0.42 -1.5 335,382 + GFF2907 0.62 -2.1 335,382 + GFF2907 0.62 -0.8 335,415 + GFF2907 0.65 +0.2 335,415 + GFF2907 0.65 +0.2 335,416 - GFF2907 0.65 -0.8 335,438 + GFF2907 0.66 +0.5 335,887 + +0.3 335,887 + -1.1 335,888 - +1.2 336,122 + GFF2908 0.23 +1.4 336,122 + GFF2908 0.23 -0.9 336,123 - GFF2908 0.23 -0.3 336,123 - GFF2908 0.23 -0.5 336,123 - GFF2908 0.23 -1.2 336,317 + GFF2908 0.42 +0.8 336,318 - GFF2908 0.42 -0.5 336,318 - GFF2908 0.42 +0.2 336,318 - GFF2908 0.42 +0.6 336,518 + GFF2908 0.62 +0.6 336,518 + GFF2908 0.62 -0.5 336,518 + GFF2908 0.62 -0.6 336,518 + GFF2908 0.62 +0.9 336,519 - GFF2908 0.62 +0.4 336,519 - GFF2908 0.62 +0.5 336,543 - GFF2908 0.65 +1.0 336,608 + GFF2908 0.71 -1.4 336,609 - GFF2908 0.71 +0.4 336,609 - GFF2908 0.71 -0.6 336,874 - -1.2
Or see this region's nucleotide sequence