Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2870 and GFF2871 are separated by 192 nucleotides GFF2871 and GFF2872 are separated by 51 nucleotides GFF2872 and GFF2873 are separated by 67 nucleotides GFF2873 and GFF2874 overlap by 4 nucleotides
GFF2870 - Nicotinate phosphoribosyltransferase (EC 6.3.4.21), at 294,653 to 295,873
GFF2870
GFF2871 - UPF0337 protein YjbJ, at 296,066 to 296,299
GFF2871
GFF2872 - Cell division protein FtsL, at 296,351 to 296,758
GFF2872
GFF2873 - Propionyl-CoA thioesterase activity, at 296,826 to 297,269
GFF2873
GFF2874 - Deacetylases, including yeast histone deacetylase and acetoin utilization protein, at 297,266 to 298,192
GFF2874
Position (kb) 296
297
298 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 295.839 kb on + strand at 295.839 kb on + strand at 295.839 kb on + strand at 295.839 kb on + strand at 295.839 kb on + strand at 295.839 kb on + strand at 295.840 kb on - strand at 295.840 kb on - strand at 295.840 kb on - strand at 295.881 kb on + strand at 295.881 kb on + strand at 295.881 kb on + strand at 295.881 kb on + strand at 295.881 kb on + strand at 295.882 kb on - strand at 295.958 kb on + strand at 295.959 kb on - strand at 296.294 kb on + strand at 296.294 kb on + strand at 296.432 kb on + strand, within GFF2872 at 296.432 kb on + strand, within GFF2872 at 296.432 kb on + strand, within GFF2872 at 296.432 kb on + strand, within GFF2872 at 296.432 kb on + strand, within GFF2872 at 296.432 kb on + strand, within GFF2872 at 296.432 kb on + strand, within GFF2872 at 296.432 kb on + strand, within GFF2872 at 296.432 kb on + strand, within GFF2872 at 296.432 kb on + strand, within GFF2872 at 296.433 kb on - strand, within GFF2872 at 296.433 kb on - strand, within GFF2872 at 296.433 kb on - strand, within GFF2872 at 296.433 kb on - strand, within GFF2872 at 296.433 kb on - strand, within GFF2872 at 296.433 kb on - strand, within GFF2872 at 296.433 kb on - strand, within GFF2872 at 296.433 kb on - strand, within GFF2872 at 296.465 kb on + strand, within GFF2872 at 296.465 kb on + strand, within GFF2872 at 296.465 kb on + strand, within GFF2872 at 296.465 kb on + strand, within GFF2872 at 296.465 kb on + strand, within GFF2872 at 296.466 kb on - strand, within GFF2872 at 296.466 kb on - strand, within GFF2872 at 296.466 kb on - strand, within GFF2872 at 296.576 kb on + strand, within GFF2872 at 296.576 kb on + strand, within GFF2872 at 296.576 kb on + strand, within GFF2872 at 296.576 kb on + strand, within GFF2872 at 296.577 kb on - strand, within GFF2872 at 296.577 kb on - strand, within GFF2872 at 296.577 kb on - strand, within GFF2872 at 296.577 kb on - strand, within GFF2872 at 296.633 kb on + strand, within GFF2872 at 296.633 kb on + strand, within GFF2872 at 296.633 kb on + strand, within GFF2872 at 296.633 kb on + strand, within GFF2872 at 296.633 kb on + strand, within GFF2872 at 296.633 kb on + strand, within GFF2872 at 296.633 kb on + strand, within GFF2872 at 296.633 kb on + strand, within GFF2872 at 296.633 kb on + strand, within GFF2872 at 296.634 kb on - strand, within GFF2872 at 296.634 kb on - strand, within GFF2872 at 296.634 kb on - strand, within GFF2872 at 296.634 kb on - strand, within GFF2872 at 296.648 kb on + strand, within GFF2872 at 296.756 kb on + strand at 296.756 kb on + strand at 296.756 kb on + strand at 296.756 kb on + strand at 296.756 kb on + strand at 296.756 kb on + strand at 296.756 kb on + strand at 296.756 kb on + strand at 296.756 kb on + strand at 296.756 kb on + strand at 296.756 kb on + strand at 296.756 kb on + strand at 296.756 kb on + strand at 296.756 kb on + strand at 296.756 kb on + strand at 296.757 kb on - strand at 296.757 kb on - strand at 296.757 kb on - strand at 296.757 kb on - strand at 296.757 kb on - strand at 296.757 kb on - strand at 296.757 kb on - strand at 296.757 kb on - strand at 296.757 kb on - strand at 296.757 kb on - strand at 296.757 kb on - strand at 296.757 kb on - strand at 296.757 kb on - strand at 296.757 kb on - strand at 296.758 kb on - strand at 296.763 kb on + strand at 296.763 kb on + strand at 296.763 kb on + strand at 296.771 kb on + strand at 296.771 kb on + strand at 296.902 kb on + strand, within GFF2873 at 296.902 kb on + strand, within GFF2873 at 296.902 kb on + strand, within GFF2873 at 296.902 kb on + strand, within GFF2873 at 296.902 kb on + strand, within GFF2873 at 296.902 kb on + strand, within GFF2873 at 296.903 kb on - strand, within GFF2873 at 296.903 kb on - strand, within GFF2873 at 296.903 kb on - strand, within GFF2873 at 296.968 kb on + strand, within GFF2873 at 296.968 kb on + strand, within GFF2873 at 297.046 kb on + strand, within GFF2873 at 297.046 kb on + strand, within GFF2873 at 297.046 kb on + strand, within GFF2873 at 297.047 kb on - strand, within GFF2873 at 297.151 kb on + strand, within GFF2873 at 297.151 kb on + strand, within GFF2873 at 297.151 kb on + strand, within GFF2873 at 297.151 kb on + strand, within GFF2873 at 297.151 kb on + strand, within GFF2873 at 297.151 kb on + strand, within GFF2873 at 297.151 kb on + strand, within GFF2873 at 297.151 kb on + strand, within GFF2873 at 297.152 kb on - strand, within GFF2873 at 297.152 kb on - strand, within GFF2873 at 297.152 kb on - strand, within GFF2873 at 297.152 kb on - strand, within GFF2873 at 297.152 kb on - strand, within GFF2873 at 297.152 kb on - strand, within GFF2873 at 297.154 kb on + strand, within GFF2873 at 297.155 kb on - strand, within GFF2873 at 297.155 kb on - strand, within GFF2873 at 297.155 kb on - strand, within GFF2873 at 297.155 kb on - strand, within GFF2873 at 297.155 kb on - strand, within GFF2873 at 297.164 kb on + strand, within GFF2873 at 297.164 kb on + strand, within GFF2873 at 297.164 kb on + strand, within GFF2873 at 297.165 kb on - strand, within GFF2873 at 297.165 kb on - strand, within GFF2873 at 297.165 kb on - strand, within GFF2873 at 297.178 kb on + strand, within GFF2873 at 297.178 kb on + strand, within GFF2873 at 297.178 kb on + strand, within GFF2873 at 297.178 kb on + strand, within GFF2873 at 297.178 kb on + strand, within GFF2873 at 297.178 kb on + strand, within GFF2873 at 297.178 kb on + strand, within GFF2873 at 297.178 kb on + strand, within GFF2873 at 297.178 kb on + strand, within GFF2873 at 297.178 kb on + strand, within GFF2873 at 297.178 kb on + strand, within GFF2873 at 297.178 kb on + strand, within GFF2873 at 297.178 kb on + strand, within GFF2873 at 297.178 kb on + strand, within GFF2873 at 297.178 kb on + strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.179 kb on - strand, within GFF2873 at 297.226 kb on + strand at 297.297 kb on + strand at 297.298 kb on - strand at 297.360 kb on + strand, within GFF2874 at 297.504 kb on + strand, within GFF2874 at 297.505 kb on - strand, within GFF2874 at 297.505 kb on - strand, within GFF2874 at 297.505 kb on - strand, within GFF2874 at 297.505 kb on - strand, within GFF2874 at 297.571 kb on - strand, within GFF2874 at 297.571 kb on - strand, within GFF2874 at 297.571 kb on - strand, within GFF2874 at 297.837 kb on + strand, within GFF2874 at 297.838 kb on - strand, within GFF2874 at 298.179 kb on + strand at 298.179 kb on + strand at 298.179 kb on + strand at 298.179 kb on + strand at 298.179 kb on + strand at 298.180 kb on - strand at 298.180 kb on - strand at 298.180 kb on - strand at 298.180 kb on - strand at 298.180 kb on - strand at 298.180 kb on - strand at 298.180 kb on - strand at 298.180 kb on - strand at 298.222 kb on + strand at 298.223 kb on - strand at 298.236 kb on + strand at 298.237 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 295,839 + -1.4 295,839 + -0.9 295,839 + +0.1 295,839 + +0.7 295,839 + -0.6 295,839 + +0.4 295,840 - -1.0 295,840 - +0.5 295,840 - -0.7 295,881 + -2.1 295,881 + +0.3 295,881 + -0.3 295,881 + -0.2 295,881 + +0.3 295,882 - -1.2 295,958 + +0.2 295,959 - -1.5 296,294 + -0.0 296,294 + -0.2 296,432 + GFF2872 0.20 +0.3 296,432 + GFF2872 0.20 -0.1 296,432 + GFF2872 0.20 -0.1 296,432 + GFF2872 0.20 +3.0 296,432 + GFF2872 0.20 -0.6 296,432 + GFF2872 0.20 -0.1 296,432 + GFF2872 0.20 +0.2 296,432 + GFF2872 0.20 -0.0 296,432 + GFF2872 0.20 -1.1 296,432 + GFF2872 0.20 +0.1 296,433 - GFF2872 0.20 +0.1 296,433 - GFF2872 0.20 +0.0 296,433 - GFF2872 0.20 -0.9 296,433 - GFF2872 0.20 -0.2 296,433 - GFF2872 0.20 +0.2 296,433 - GFF2872 0.20 +0.3 296,433 - GFF2872 0.20 -0.6 296,433 - GFF2872 0.20 +0.4 296,465 + GFF2872 0.28 +1.8 296,465 + GFF2872 0.28 -0.3 296,465 + GFF2872 0.28 -0.4 296,465 + GFF2872 0.28 +0.0 296,465 + GFF2872 0.28 -0.6 296,466 - GFF2872 0.28 -0.6 296,466 - GFF2872 0.28 +0.2 296,466 - GFF2872 0.28 +2.1 296,576 + GFF2872 0.55 +1.7 296,576 + GFF2872 0.55 -0.7 296,576 + GFF2872 0.55 -0.1 296,576 + GFF2872 0.55 -0.9 296,577 - GFF2872 0.55 -1.6 296,577 - GFF2872 0.55 +0.7 296,577 - GFF2872 0.55 +0.2 296,577 - GFF2872 0.55 +0.3 296,633 + GFF2872 0.69 -0.1 296,633 + GFF2872 0.69 +0.4 296,633 + GFF2872 0.69 +0.6 296,633 + GFF2872 0.69 +1.1 296,633 + GFF2872 0.69 +0.3 296,633 + GFF2872 0.69 +0.2 296,633 + GFF2872 0.69 +0.0 296,633 + GFF2872 0.69 -0.6 296,633 + GFF2872 0.69 -0.1 296,634 - GFF2872 0.69 +0.9 296,634 - GFF2872 0.69 +0.8 296,634 - GFF2872 0.69 -0.4 296,634 - GFF2872 0.69 +0.4 296,648 + GFF2872 0.73 +0.6 296,756 + -1.2 296,756 + +0.3 296,756 + +0.2 296,756 + -0.2 296,756 + +0.1 296,756 + +0.2 296,756 + +0.3 296,756 + -0.2 296,756 + +0.8 296,756 + -0.0 296,756 + +0.4 296,756 + +0.7 296,756 + -1.8 296,756 + -0.3 296,756 + +0.2 296,757 - -1.3 296,757 - +0.2 296,757 - +0.3 296,757 - -0.5 296,757 - +0.2 296,757 - +0.8 296,757 - -0.3 296,757 - +0.4 296,757 - +0.5 296,757 - -1.3 296,757 - -1.3 296,757 - -0.2 296,757 - -0.2 296,757 - +0.3 296,758 - -0.7 296,763 + -0.5 296,763 + +1.1 296,763 + +0.8 296,771 + -0.5 296,771 + +0.9 296,902 + GFF2873 0.17 -0.9 296,902 + GFF2873 0.17 -0.1 296,902 + GFF2873 0.17 -0.9 296,902 + GFF2873 0.17 -0.4 296,902 + GFF2873 0.17 -0.2 296,902 + GFF2873 0.17 +0.5 296,903 - GFF2873 0.17 -0.7 296,903 - GFF2873 0.17 +1.7 296,903 - GFF2873 0.17 -2.7 296,968 + GFF2873 0.32 -0.4 296,968 + GFF2873 0.32 -1.6 297,046 + GFF2873 0.50 -0.0 297,046 + GFF2873 0.50 -0.8 297,046 + GFF2873 0.50 -0.7 297,047 - GFF2873 0.50 -2.0 297,151 + GFF2873 0.73 +0.8 297,151 + GFF2873 0.73 -0.7 297,151 + GFF2873 0.73 -0.4 297,151 + GFF2873 0.73 -2.2 297,151 + GFF2873 0.73 +0.5 297,151 + GFF2873 0.73 -0.1 297,151 + GFF2873 0.73 -0.1 297,151 + GFF2873 0.73 -0.5 297,152 - GFF2873 0.73 -0.1 297,152 - GFF2873 0.73 +0.3 297,152 - GFF2873 0.73 -0.0 297,152 - GFF2873 0.73 +2.0 297,152 - GFF2873 0.73 -0.1 297,152 - GFF2873 0.73 -0.1 297,154 + GFF2873 0.74 -0.1 297,155 - GFF2873 0.74 +1.0 297,155 - GFF2873 0.74 -2.0 297,155 - GFF2873 0.74 -0.3 297,155 - GFF2873 0.74 +1.0 297,155 - GFF2873 0.74 -0.4 297,164 + GFF2873 0.76 +0.0 297,164 + GFF2873 0.76 -0.4 297,164 + GFF2873 0.76 +1.8 297,165 - GFF2873 0.76 -0.0 297,165 - GFF2873 0.76 +3.1 297,165 - GFF2873 0.76 -0.9 297,178 + GFF2873 0.79 -0.6 297,178 + GFF2873 0.79 -0.2 297,178 + GFF2873 0.79 +0.1 297,178 + GFF2873 0.79 -1.1 297,178 + GFF2873 0.79 -0.9 297,178 + GFF2873 0.79 -1.8 297,178 + GFF2873 0.79 -1.1 297,178 + GFF2873 0.79 +0.3 297,178 + GFF2873 0.79 +0.4 297,178 + GFF2873 0.79 -1.2 297,178 + GFF2873 0.79 -0.5 297,178 + GFF2873 0.79 +0.0 297,178 + GFF2873 0.79 +0.1 297,178 + GFF2873 0.79 -0.4 297,178 + GFF2873 0.79 -0.5 297,179 - GFF2873 0.80 -2.4 297,179 - GFF2873 0.80 +0.9 297,179 - GFF2873 0.80 +0.7 297,179 - GFF2873 0.80 +0.3 297,179 - GFF2873 0.80 -0.3 297,179 - GFF2873 0.80 +0.1 297,179 - GFF2873 0.80 -0.1 297,179 - GFF2873 0.80 +0.5 297,179 - GFF2873 0.80 -0.0 297,179 - GFF2873 0.80 +0.3 297,179 - GFF2873 0.80 -0.2 297,179 - GFF2873 0.80 +2.1 297,179 - GFF2873 0.80 -0.1 297,179 - GFF2873 0.80 -0.9 297,179 - GFF2873 0.80 -0.3 297,179 - GFF2873 0.80 -0.6 297,179 - GFF2873 0.80 -0.2 297,179 - GFF2873 0.80 -0.8 297,179 - GFF2873 0.80 +0.6 297,179 - GFF2873 0.80 +0.2 297,179 - GFF2873 0.80 -0.6 297,179 - GFF2873 0.80 -1.0 297,179 - GFF2873 0.80 -1.1 297,226 + +0.1 297,297 + -0.3 297,298 - -0.0 297,360 + GFF2874 0.10 -1.1 297,504 + GFF2874 0.26 -0.0 297,505 - GFF2874 0.26 -0.0 297,505 - GFF2874 0.26 +0.4 297,505 - GFF2874 0.26 +0.1 297,505 - GFF2874 0.26 +0.9 297,571 - GFF2874 0.33 +0.6 297,571 - GFF2874 0.33 -0.2 297,571 - GFF2874 0.33 +2.1 297,837 + GFF2874 0.62 +0.1 297,838 - GFF2874 0.62 +1.7 298,179 + +0.4 298,179 + -0.5 298,179 + -0.0 298,179 + -1.1 298,179 + +1.1 298,180 - -0.1 298,180 - -0.2 298,180 - -0.9 298,180 - -0.1 298,180 - -1.1 298,180 - -1.3 298,180 - -0.8 298,180 - +0.3 298,222 + +0.2 298,223 - -0.4 298,236 + -0.5 298,237 - +0.5
Or see this region's nucleotide sequence