Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2764 and GFF2765 are separated by 37 nucleotides GFF2765 and GFF2766 overlap by 4 nucleotides GFF2766 and GFF2767 are separated by 9 nucleotides GFF2767 and GFF2768 are separated by 38 nucleotides
GFF2764 - Carbonic anhydrase-like protein MJ0304, at 195,498 to 196,022
GFF2764
GFF2765 - FIG00005326: uncharacterized protein, at 196,060 to 196,872
GFF2765
GFF2766 - Pentapeptide repeat family protein, at 196,869 to 197,555
GFF2766
GFF2767 - MaoC domain protein dehydratase, at 197,565 to 198,044
GFF2767
GFF2768 - 3-ketoacyl-CoA thiolase 2 [fadN-fadA-fadE operon] (EC 2.3.1.16), at 198,083 to 199,216
GFF2768
Position (kb) 196
197
198 Strain fitness (log2 ratio) -2
-1
0
1
2 at 195.912 kb on + strand, within GFF2764 at 195.912 kb on + strand, within GFF2764 at 195.912 kb on + strand, within GFF2764 at 195.913 kb on - strand, within GFF2764 at 195.913 kb on - strand, within GFF2764 at 195.913 kb on - strand, within GFF2764 at 195.913 kb on - strand, within GFF2764 at 195.913 kb on - strand, within GFF2764 at 195.913 kb on - strand, within GFF2764 at 196.012 kb on + strand at 196.012 kb on + strand at 196.012 kb on + strand at 196.013 kb on - strand at 196.013 kb on - strand at 196.047 kb on + strand at 196.055 kb on + strand at 196.055 kb on + strand at 196.055 kb on + strand at 196.055 kb on + strand at 196.055 kb on + strand at 196.055 kb on + strand at 196.055 kb on + strand at 196.055 kb on + strand at 196.056 kb on - strand at 196.056 kb on - strand at 196.056 kb on - strand at 196.056 kb on - strand at 196.056 kb on - strand at 196.056 kb on - strand at 196.378 kb on + strand, within GFF2765 at 196.378 kb on + strand, within GFF2765 at 196.378 kb on + strand, within GFF2765 at 196.378 kb on + strand, within GFF2765 at 196.378 kb on + strand, within GFF2765 at 196.379 kb on - strand, within GFF2765 at 196.379 kb on - strand, within GFF2765 at 196.379 kb on - strand, within GFF2765 at 196.382 kb on - strand, within GFF2765 at 196.382 kb on - strand, within GFF2765 at 196.459 kb on + strand, within GFF2765 at 196.466 kb on - strand, within GFF2765 at 196.702 kb on + strand, within GFF2765 at 196.703 kb on - strand, within GFF2765 at 196.703 kb on - strand, within GFF2765 at 196.747 kb on + strand, within GFF2765 at 196.748 kb on - strand, within GFF2765 at 196.768 kb on + strand, within GFF2765 at 198.004 kb on + strand at 198.004 kb on + strand at 198.005 kb on - strand at 198.005 kb on - strand at 198.005 kb on - strand at 198.005 kb on - strand at 198.005 kb on - strand at 198.147 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 195,912 + GFF2764 0.79 -0.0 195,912 + GFF2764 0.79 -0.2 195,912 + GFF2764 0.79 +0.1 195,913 - GFF2764 0.79 +2.2 195,913 - GFF2764 0.79 +1.2 195,913 - GFF2764 0.79 -1.0 195,913 - GFF2764 0.79 +1.5 195,913 - GFF2764 0.79 +0.3 195,913 - GFF2764 0.79 +0.1 196,012 + +1.7 196,012 + -2.1 196,012 + +0.1 196,013 - -1.0 196,013 - +1.1 196,047 + -0.5 196,055 + -0.2 196,055 + -0.5 196,055 + -0.8 196,055 + +2.7 196,055 + +1.2 196,055 + +0.4 196,055 + -0.3 196,055 + +0.1 196,056 - +0.6 196,056 - +0.7 196,056 - +0.4 196,056 - +0.4 196,056 - +0.1 196,056 - +0.7 196,378 + GFF2765 0.39 +0.3 196,378 + GFF2765 0.39 -0.2 196,378 + GFF2765 0.39 -0.4 196,378 + GFF2765 0.39 -0.6 196,378 + GFF2765 0.39 -0.3 196,379 - GFF2765 0.39 +0.2 196,379 - GFF2765 0.39 +0.2 196,379 - GFF2765 0.39 -0.5 196,382 - GFF2765 0.40 +0.2 196,382 - GFF2765 0.40 +0.7 196,459 + GFF2765 0.49 +1.0 196,466 - GFF2765 0.50 +0.2 196,702 + GFF2765 0.79 +1.2 196,703 - GFF2765 0.79 -0.3 196,703 - GFF2765 0.79 -2.3 196,747 + GFF2765 0.85 -0.1 196,748 - GFF2765 0.85 +0.2 196,768 + GFF2765 0.87 +0.1 198,004 + +1.3 198,004 + +1.6 198,005 - -0.2 198,005 - +0.6 198,005 - -0.4 198,005 - -0.2 198,005 - -0.7 198,147 + -0.8
Or see this region's nucleotide sequence