Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2717 and GFF2718 overlap by 1 nucleotides GFF2718 and GFF2719 are separated by 36 nucleotides GFF2719 and GFF2720 are separated by 59 nucleotides GFF2720 and GFF2721 are separated by 28 nucleotides
GFF2717 - Epimerase KguE, at 150,761 to 151,510
GFF2717
GFF2718 - ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines), at 151,510 to 152,265
GFF2718
GFF2719 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 152,302 to 153,099
GFF2719
GFF2720 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 153,159 to 153,812
GFF2720
GFF2721 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 153,841 to 154,512
GFF2721
Position (kb) 152
153
154 Strain fitness (log2 ratio) -2
-1
0
1 at 151.329 kb on + strand, within GFF2717 at 151.329 kb on + strand, within GFF2717 at 151.330 kb on - strand, within GFF2717 at 151.853 kb on + strand, within GFF2718 at 151.853 kb on + strand, within GFF2718 at 151.892 kb on + strand, within GFF2718 at 151.892 kb on + strand, within GFF2718 at 151.892 kb on + strand, within GFF2718 at 152.219 kb on + strand at 152.219 kb on + strand at 152.220 kb on - strand at 152.292 kb on - strand at 152.303 kb on + strand at 152.304 kb on - strand at 152.471 kb on + strand, within GFF2719 at 152.711 kb on + strand, within GFF2719 at 152.711 kb on + strand, within GFF2719 at 152.910 kb on - strand, within GFF2719 at 152.945 kb on + strand, within GFF2719 at 153.104 kb on + strand at 153.104 kb on + strand at 153.104 kb on + strand at 153.104 kb on + strand at 153.105 kb on - strand at 153.105 kb on - strand at 153.105 kb on - strand at 153.105 kb on - strand at 153.105 kb on - strand at 153.105 kb on - strand at 153.235 kb on + strand, within GFF2720 at 153.235 kb on + strand, within GFF2720 at 153.235 kb on + strand, within GFF2720 at 153.236 kb on - strand, within GFF2720 at 153.367 kb on + strand, within GFF2720 at 153.367 kb on + strand, within GFF2720 at 153.368 kb on - strand, within GFF2720 at 153.368 kb on - strand, within GFF2720 at 153.368 kb on - strand, within GFF2720 at 153.368 kb on - strand, within GFF2720 at 153.436 kb on + strand, within GFF2720 at 153.436 kb on + strand, within GFF2720 at 153.436 kb on + strand, within GFF2720 at 153.436 kb on + strand, within GFF2720 at 153.436 kb on + strand, within GFF2720 at 153.437 kb on - strand, within GFF2720 at 153.437 kb on - strand, within GFF2720 at 153.437 kb on - strand, within GFF2720 at 153.437 kb on - strand, within GFF2720 at 153.437 kb on - strand, within GFF2720 at 153.437 kb on - strand, within GFF2720 at 153.437 kb on - strand, within GFF2720 at 153.437 kb on - strand, within GFF2720 at 153.806 kb on + strand at 153.806 kb on + strand at 153.806 kb on + strand at 153.806 kb on + strand at 153.806 kb on + strand at 153.807 kb on - strand at 153.807 kb on - strand at 153.807 kb on - strand at 153.857 kb on + strand at 153.876 kb on - strand at 153.920 kb on + strand, within GFF2721 at 153.920 kb on + strand, within GFF2721 at 153.920 kb on + strand, within GFF2721 at 153.920 kb on + strand, within GFF2721 at 153.920 kb on + strand, within GFF2721 at 153.920 kb on + strand, within GFF2721 at 153.920 kb on + strand, within GFF2721 at 153.920 kb on + strand, within GFF2721 at 153.921 kb on - strand, within GFF2721 at 153.921 kb on - strand, within GFF2721 at 153.921 kb on - strand, within GFF2721 at 153.921 kb on - strand, within GFF2721 at 153.941 kb on + strand, within GFF2721 at 153.942 kb on - strand, within GFF2721 at 153.942 kb on - strand, within GFF2721 at 154.070 kb on + strand, within GFF2721
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 151,329 + GFF2717 0.76 -0.5 151,329 + GFF2717 0.76 -0.7 151,330 - GFF2717 0.76 -1.7 151,853 + GFF2718 0.45 -0.4 151,853 + GFF2718 0.45 -1.4 151,892 + GFF2718 0.51 -0.9 151,892 + GFF2718 0.51 -0.8 151,892 + GFF2718 0.51 +0.1 152,219 + -0.9 152,219 + +1.0 152,220 - +0.4 152,292 - +1.2 152,303 + +1.3 152,304 - -0.1 152,471 + GFF2719 0.21 -1.8 152,711 + GFF2719 0.51 -1.8 152,711 + GFF2719 0.51 -1.1 152,910 - GFF2719 0.76 -0.1 152,945 + GFF2719 0.81 -2.6 153,104 + +1.0 153,104 + -0.6 153,104 + +0.7 153,104 + -1.7 153,105 - -1.1 153,105 - -1.0 153,105 - -0.2 153,105 - +1.1 153,105 - +1.1 153,105 - +0.3 153,235 + GFF2720 0.12 +0.4 153,235 + GFF2720 0.12 +0.5 153,235 + GFF2720 0.12 +1.1 153,236 - GFF2720 0.12 -1.2 153,367 + GFF2720 0.32 +0.1 153,367 + GFF2720 0.32 -0.0 153,368 - GFF2720 0.32 +0.1 153,368 - GFF2720 0.32 -0.4 153,368 - GFF2720 0.32 -0.8 153,368 - GFF2720 0.32 +0.1 153,436 + GFF2720 0.42 +0.4 153,436 + GFF2720 0.42 -0.3 153,436 + GFF2720 0.42 +0.4 153,436 + GFF2720 0.42 -2.3 153,436 + GFF2720 0.42 +0.5 153,437 - GFF2720 0.43 +0.9 153,437 - GFF2720 0.43 -0.6 153,437 - GFF2720 0.43 -0.1 153,437 - GFF2720 0.43 +1.6 153,437 - GFF2720 0.43 +0.6 153,437 - GFF2720 0.43 -0.2 153,437 - GFF2720 0.43 +1.1 153,437 - GFF2720 0.43 -0.6 153,806 + -1.4 153,806 + -0.7 153,806 + +0.4 153,806 + -0.2 153,806 + -0.6 153,807 - -0.1 153,807 - +0.3 153,807 - -0.2 153,857 + -0.8 153,876 - +0.1 153,920 + GFF2721 0.12 +0.3 153,920 + GFF2721 0.12 +0.9 153,920 + GFF2721 0.12 +0.0 153,920 + GFF2721 0.12 -0.2 153,920 + GFF2721 0.12 -0.7 153,920 + GFF2721 0.12 -0.4 153,920 + GFF2721 0.12 -1.0 153,920 + GFF2721 0.12 -0.6 153,921 - GFF2721 0.12 +0.2 153,921 - GFF2721 0.12 -0.3 153,921 - GFF2721 0.12 -0.6 153,921 - GFF2721 0.12 -0.7 153,941 + GFF2721 0.15 -1.7 153,942 - GFF2721 0.15 -0.2 153,942 - GFF2721 0.15 -0.1 154,070 + GFF2721 0.34 -0.7
Or see this region's nucleotide sequence