Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2676 and GFF2677 are separated by 61 nucleotides GFF2677 and GFF2678 are separated by 189 nucleotides GFF2678 and GFF2679 are separated by 24 nucleotides
GFF2676 - Dialkylrecorsinol condensing enzyme, at 97,712 to 98,770
GFF2676
GFF2677 - Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3), at 98,832 to 100,415
GFF2677
GFF2678 - Glycerol-3-phosphate regulon repressor GlpR, at 100,605 to 101,375
GFF2678
GFF2679 - Glycerol kinase (EC 2.7.1.30), at 101,400 to 102,896
GFF2679
Position (kb) 98
99
100
101 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 97.852 kb on + strand, within GFF2676 at 97.856 kb on + strand, within GFF2676 at 97.856 kb on + strand, within GFF2676 at 97.856 kb on + strand, within GFF2676 at 97.875 kb on + strand, within GFF2676 at 97.875 kb on + strand, within GFF2676 at 97.875 kb on + strand, within GFF2676 at 97.875 kb on + strand, within GFF2676 at 97.875 kb on + strand, within GFF2676 at 97.875 kb on + strand, within GFF2676 at 97.875 kb on + strand, within GFF2676 at 97.875 kb on + strand, within GFF2676 at 97.876 kb on - strand, within GFF2676 at 97.876 kb on - strand, within GFF2676 at 97.876 kb on - strand, within GFF2676 at 97.876 kb on - strand, within GFF2676 at 97.876 kb on - strand, within GFF2676 at 97.876 kb on - strand, within GFF2676 at 97.947 kb on + strand, within GFF2676 at 97.948 kb on - strand, within GFF2676 at 97.948 kb on - strand, within GFF2676 at 98.401 kb on - strand, within GFF2676 at 98.746 kb on + strand at 98.747 kb on - strand at 98.986 kb on + strand at 98.986 kb on + strand at 98.986 kb on + strand at 98.987 kb on - strand at 99.097 kb on + strand, within GFF2677 at 99.098 kb on - strand, within GFF2677 at 99.358 kb on + strand, within GFF2677 at 99.359 kb on - strand, within GFF2677 at 99.359 kb on - strand, within GFF2677 at 99.359 kb on - strand, within GFF2677 at 99.359 kb on - strand, within GFF2677 at 99.422 kb on - strand, within GFF2677 at 99.422 kb on - strand, within GFF2677 at 99.422 kb on - strand, within GFF2677 at 99.430 kb on - strand, within GFF2677 at 99.464 kb on - strand, within GFF2677 at 99.487 kb on + strand, within GFF2677 at 99.487 kb on + strand, within GFF2677 at 99.488 kb on - strand, within GFF2677 at 99.625 kb on + strand, within GFF2677 at 99.625 kb on + strand, within GFF2677 at 99.625 kb on + strand, within GFF2677 at 99.625 kb on + strand, within GFF2677 at 99.625 kb on + strand, within GFF2677 at 99.625 kb on + strand, within GFF2677 at 99.626 kb on - strand, within GFF2677 at 99.626 kb on - strand, within GFF2677 at 99.626 kb on - strand, within GFF2677 at 99.626 kb on - strand, within GFF2677 at 99.626 kb on - strand, within GFF2677 at 99.626 kb on - strand, within GFF2677 at 99.626 kb on - strand, within GFF2677 at 99.952 kb on + strand, within GFF2677 at 99.953 kb on - strand, within GFF2677 at 99.953 kb on - strand, within GFF2677 at 99.953 kb on - strand, within GFF2677 at 99.970 kb on + strand, within GFF2677 at 99.970 kb on + strand, within GFF2677 at 99.970 kb on + strand, within GFF2677 at 99.971 kb on - strand, within GFF2677 at 100.060 kb on + strand, within GFF2677 at 100.060 kb on + strand, within GFF2677 at 100.060 kb on + strand, within GFF2677 at 100.060 kb on + strand, within GFF2677 at 100.060 kb on + strand, within GFF2677 at 100.060 kb on + strand, within GFF2677 at 100.061 kb on - strand, within GFF2677 at 100.061 kb on - strand, within GFF2677 at 100.061 kb on - strand, within GFF2677 at 100.061 kb on - strand, within GFF2677 at 100.061 kb on - strand, within GFF2677 at 100.061 kb on - strand, within GFF2677 at 100.061 kb on - strand, within GFF2677 at 100.061 kb on - strand, within GFF2677 at 100.061 kb on - strand, within GFF2677 at 100.061 kb on - strand, within GFF2677 at 100.210 kb on + strand, within GFF2677 at 100.211 kb on - strand, within GFF2677 at 100.211 kb on - strand, within GFF2677 at 100.424 kb on + strand at 100.424 kb on + strand at 100.424 kb on + strand at 100.424 kb on + strand at 100.424 kb on + strand at 100.425 kb on - strand at 100.425 kb on - strand at 100.425 kb on - strand at 100.425 kb on - strand at 100.432 kb on - strand at 100.466 kb on + strand at 100.467 kb on - strand at 100.549 kb on + strand at 100.550 kb on - strand at 100.566 kb on + strand at 100.566 kb on + strand at 100.566 kb on + strand at 100.566 kb on + strand at 100.567 kb on - strand at 100.567 kb on - strand at 100.723 kb on + strand, within GFF2678 at 100.723 kb on + strand, within GFF2678 at 100.723 kb on + strand, within GFF2678 at 100.723 kb on + strand, within GFF2678 at 100.723 kb on + strand, within GFF2678 at 100.724 kb on - strand, within GFF2678 at 100.806 kb on + strand, within GFF2678 at 100.873 kb on + strand, within GFF2678 at 101.173 kb on + strand, within GFF2678 at 101.173 kb on + strand, within GFF2678 at 101.174 kb on - strand, within GFF2678 at 101.174 kb on - strand, within GFF2678 at 101.409 kb on + strand at 101.409 kb on + strand at 101.409 kb on + strand at 101.409 kb on + strand at 101.409 kb on + strand at 101.409 kb on + strand at 101.410 kb on - strand at 101.410 kb on - strand at 101.410 kb on - strand at 101.410 kb on - strand at 101.410 kb on - strand at 101.410 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 97,852 + GFF2676 0.13 +0.2 97,856 + GFF2676 0.14 +0.5 97,856 + GFF2676 0.14 -0.3 97,856 + GFF2676 0.14 -1.2 97,875 + GFF2676 0.15 -1.2 97,875 + GFF2676 0.15 +0.6 97,875 + GFF2676 0.15 -0.3 97,875 + GFF2676 0.15 -1.4 97,875 + GFF2676 0.15 +1.3 97,875 + GFF2676 0.15 -0.8 97,875 + GFF2676 0.15 -0.2 97,875 + GFF2676 0.15 -0.1 97,876 - GFF2676 0.15 -2.4 97,876 - GFF2676 0.15 -0.3 97,876 - GFF2676 0.15 -0.1 97,876 - GFF2676 0.15 +0.5 97,876 - GFF2676 0.15 -0.3 97,876 - GFF2676 0.15 -0.2 97,947 + GFF2676 0.22 +0.5 97,948 - GFF2676 0.22 +0.0 97,948 - GFF2676 0.22 +0.3 98,401 - GFF2676 0.65 +0.5 98,746 + +0.3 98,747 - -0.2 98,986 + -1.9 98,986 + -1.1 98,986 + -1.5 98,987 - -0.5 99,097 + GFF2677 0.17 +1.6 99,098 - GFF2677 0.17 -1.7 99,358 + GFF2677 0.33 -1.4 99,359 - GFF2677 0.33 -1.5 99,359 - GFF2677 0.33 +0.4 99,359 - GFF2677 0.33 +0.6 99,359 - GFF2677 0.33 +0.6 99,422 - GFF2677 0.37 -1.7 99,422 - GFF2677 0.37 +1.6 99,422 - GFF2677 0.37 -2.4 99,430 - GFF2677 0.38 -1.6 99,464 - GFF2677 0.40 -1.2 99,487 + GFF2677 0.41 -1.2 99,487 + GFF2677 0.41 +0.2 99,488 - GFF2677 0.41 -0.7 99,625 + GFF2677 0.50 -2.0 99,625 + GFF2677 0.50 +1.6 99,625 + GFF2677 0.50 -0.5 99,625 + GFF2677 0.50 -1.6 99,625 + GFF2677 0.50 -0.4 99,625 + GFF2677 0.50 -0.8 99,626 - GFF2677 0.50 -0.2 99,626 - GFF2677 0.50 +0.6 99,626 - GFF2677 0.50 -2.2 99,626 - GFF2677 0.50 -2.1 99,626 - GFF2677 0.50 -2.8 99,626 - GFF2677 0.50 -2.4 99,626 - GFF2677 0.50 +0.3 99,952 + GFF2677 0.71 +1.0 99,953 - GFF2677 0.71 -0.1 99,953 - GFF2677 0.71 -0.0 99,953 - GFF2677 0.71 -0.8 99,970 + GFF2677 0.72 -1.2 99,970 + GFF2677 0.72 +1.2 99,970 + GFF2677 0.72 -1.4 99,971 - GFF2677 0.72 -0.8 100,060 + GFF2677 0.78 -0.8 100,060 + GFF2677 0.78 -0.7 100,060 + GFF2677 0.78 +0.8 100,060 + GFF2677 0.78 -1.4 100,060 + GFF2677 0.78 -1.2 100,060 + GFF2677 0.78 -1.4 100,061 - GFF2677 0.78 +0.4 100,061 - GFF2677 0.78 -0.4 100,061 - GFF2677 0.78 -0.6 100,061 - GFF2677 0.78 -1.4 100,061 - GFF2677 0.78 +0.1 100,061 - GFF2677 0.78 -0.9 100,061 - GFF2677 0.78 -1.5 100,061 - GFF2677 0.78 +1.1 100,061 - GFF2677 0.78 -1.5 100,061 - GFF2677 0.78 +0.6 100,210 + GFF2677 0.87 -0.4 100,211 - GFF2677 0.87 -0.7 100,211 - GFF2677 0.87 -2.5 100,424 + -0.3 100,424 + +0.1 100,424 + -2.0 100,424 + -1.4 100,424 + -1.4 100,425 - +0.1 100,425 - -0.1 100,425 - +0.0 100,425 - +0.5 100,432 - -0.5 100,466 + -0.1 100,467 - +1.1 100,549 + -1.0 100,550 - +0.0 100,566 + -0.4 100,566 + +0.2 100,566 + -0.0 100,566 + -1.4 100,567 - +0.2 100,567 - -0.7 100,723 + GFF2678 0.15 -1.1 100,723 + GFF2678 0.15 +0.1 100,723 + GFF2678 0.15 +0.8 100,723 + GFF2678 0.15 -1.8 100,723 + GFF2678 0.15 -1.9 100,724 - GFF2678 0.15 +0.8 100,806 + GFF2678 0.26 -0.4 100,873 + GFF2678 0.35 +0.4 101,173 + GFF2678 0.74 +1.4 101,173 + GFF2678 0.74 -2.8 101,174 - GFF2678 0.74 -1.2 101,174 - GFF2678 0.74 -0.8 101,409 + +0.8 101,409 + -0.4 101,409 + +0.1 101,409 + -0.7 101,409 + -1.1 101,409 + +0.3 101,410 - -0.1 101,410 - +0.2 101,410 - +0.2 101,410 - +1.3 101,410 - -0.9 101,410 - +0.1
Or see this region's nucleotide sequence