Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2576 and GFF2577 are separated by 40 nucleotides GFF2577 and GFF2578 overlap by 4 nucleotides GFF2578 and GFF2579 are separated by 19 nucleotides GFF2579 and GFF2580 are separated by 69 nucleotides
GFF2576 - 6-carboxy-5,6,7,8-tetrahydropterin synthase (EC 4.1.2.50), at 633,652 to 634,014
GFF2576
GFF2577 - 7-carboxy-7-deazaguanine synthase (EC 4.3.99.3), at 634,055 to 634,693
GFF2577
GFF2578 - Pantoate--beta-alanine ligase (EC 6.3.2.1), at 634,690 to 635,532
GFF2578
GFF2579 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11), at 635,552 to 636,445
GFF2579
GFF2580 - L-aspartate oxidase (EC 1.4.3.16), at 636,515 to 638,101
GFF2580
Position (kb) 634
635
636 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.915 kb on - strand, within GFF2576 at 633.915 kb on - strand, within GFF2576 at 633.915 kb on - strand, within GFF2576 at 633.915 kb on - strand, within GFF2576 at 633.915 kb on - strand, within GFF2576 at 633.930 kb on + strand, within GFF2576 at 633.930 kb on + strand, within GFF2576 at 633.931 kb on - strand, within GFF2576 at 634.665 kb on + strand at 634.665 kb on + strand at 634.665 kb on + strand at 634.665 kb on + strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.686 kb on + strand at 634.686 kb on + strand at 634.686 kb on + strand at 634.686 kb on + strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.823 kb on + strand, within GFF2578 at 634.823 kb on + strand, within GFF2578 at 634.823 kb on + strand, within GFF2578 at 634.823 kb on + strand, within GFF2578 at 634.824 kb on - strand, within GFF2578 at 634.824 kb on - strand, within GFF2578 at 634.824 kb on - strand, within GFF2578 at 634.824 kb on - strand, within GFF2578 at 634.824 kb on - strand, within GFF2578 at 634.824 kb on - strand, within GFF2578 at 634.873 kb on + strand, within GFF2578 at 634.874 kb on - strand, within GFF2578 at 634.874 kb on - strand, within GFF2578 at 634.874 kb on - strand, within GFF2578 at 634.973 kb on + strand, within GFF2578 at 634.974 kb on - strand, within GFF2578 at 635.090 kb on + strand, within GFF2578 at 635.090 kb on + strand, within GFF2578 at 635.090 kb on + strand, within GFF2578 at 635.090 kb on + strand, within GFF2578 at 635.090 kb on + strand, within GFF2578 at 635.091 kb on - strand, within GFF2578 at 635.091 kb on - strand, within GFF2578 at 635.091 kb on - strand, within GFF2578 at 635.091 kb on - strand, within GFF2578 at 635.091 kb on - strand, within GFF2578 at 635.334 kb on - strand, within GFF2578 at 635.334 kb on - strand, within GFF2578 at 635.334 kb on - strand, within GFF2578 at 635.334 kb on - strand, within GFF2578 at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.529 kb on - strand at 635.529 kb on - strand at 635.529 kb on - strand at 635.529 kb on - strand at 635.529 kb on - strand at 635.529 kb on - strand at 635.529 kb on - strand at 635.529 kb on - strand at 635.554 kb on - strand at 635.554 kb on - strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 636.084 kb on + strand, within GFF2579 at 636.084 kb on + strand, within GFF2579 at 636.085 kb on - strand, within GFF2579 at 636.165 kb on + strand, within GFF2579 at 636.165 kb on + strand, within GFF2579 at 636.165 kb on + strand, within GFF2579 at 636.166 kb on - strand, within GFF2579 at 636.291 kb on + strand, within GFF2579 at 636.291 kb on + strand, within GFF2579 at 636.292 kb on - strand, within GFF2579 at 636.292 kb on - strand, within GFF2579 at 636.292 kb on - strand, within GFF2579 at 636.399 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 633,914 + GFF2576 0.72 -0.1 633,914 + GFF2576 0.72 -0.5 633,914 + GFF2576 0.72 +0.1 633,914 + GFF2576 0.72 +0.7 633,914 + GFF2576 0.72 -0.6 633,914 + GFF2576 0.72 -0.2 633,914 + GFF2576 0.72 -0.5 633,914 + GFF2576 0.72 -0.2 633,914 + GFF2576 0.72 -0.1 633,915 - GFF2576 0.72 +1.6 633,915 - GFF2576 0.72 -0.2 633,915 - GFF2576 0.72 +0.6 633,915 - GFF2576 0.72 -0.6 633,915 - GFF2576 0.72 -1.6 633,930 + GFF2576 0.77 -0.2 633,930 + GFF2576 0.77 -0.8 633,931 - GFF2576 0.77 +0.5 634,665 + +1.4 634,665 + -2.1 634,665 + +1.2 634,665 + -0.4 634,666 - +0.9 634,666 - -0.2 634,666 - -0.2 634,666 - +0.3 634,666 - -0.2 634,666 - +0.7 634,666 - -0.3 634,666 - -1.0 634,666 - +2.2 634,666 - -0.3 634,686 + -0.5 634,686 + -0.1 634,686 + -0.4 634,686 + -0.3 634,687 - -0.8 634,687 - +1.2 634,687 - -0.1 634,687 - +0.6 634,687 - -1.0 634,687 - +0.2 634,687 - +1.3 634,687 - -0.5 634,687 - -0.7 634,823 + GFF2578 0.16 -1.8 634,823 + GFF2578 0.16 -1.1 634,823 + GFF2578 0.16 -1.1 634,823 + GFF2578 0.16 -0.7 634,824 - GFF2578 0.16 -2.4 634,824 - GFF2578 0.16 -1.0 634,824 - GFF2578 0.16 -1.2 634,824 - GFF2578 0.16 +0.2 634,824 - GFF2578 0.16 -1.3 634,824 - GFF2578 0.16 -1.2 634,873 + GFF2578 0.22 -2.7 634,874 - GFF2578 0.22 -0.4 634,874 - GFF2578 0.22 -0.5 634,874 - GFF2578 0.22 -0.8 634,973 + GFF2578 0.34 -1.0 634,974 - GFF2578 0.34 -0.3 635,090 + GFF2578 0.47 -1.5 635,090 + GFF2578 0.47 -2.6 635,090 + GFF2578 0.47 -2.3 635,090 + GFF2578 0.47 -1.0 635,090 + GFF2578 0.47 -3.0 635,091 - GFF2578 0.48 -1.0 635,091 - GFF2578 0.48 -2.0 635,091 - GFF2578 0.48 -1.8 635,091 - GFF2578 0.48 -1.4 635,091 - GFF2578 0.48 -1.4 635,334 - GFF2578 0.76 -1.0 635,334 - GFF2578 0.76 -1.6 635,334 - GFF2578 0.76 -0.8 635,334 - GFF2578 0.76 -1.8 635,528 + -2.2 635,528 + -1.8 635,528 + -1.3 635,528 + -1.8 635,528 + -1.9 635,528 + -1.1 635,528 + -2.4 635,528 + +0.9 635,528 + -1.0 635,528 + -0.5 635,529 - -1.6 635,529 - -0.5 635,529 - -1.3 635,529 - +1.8 635,529 - -2.4 635,529 - -1.2 635,529 - -2.0 635,529 - -0.3 635,554 - -1.0 635,554 - -0.9 635,607 + -0.1 635,607 + -0.6 635,607 + -0.3 635,607 + +0.5 635,607 + +0.1 635,607 + -1.3 635,607 + -1.2 635,607 + -0.4 635,607 + -1.0 635,607 + +0.9 635,607 + -0.8 635,607 + -0.6 635,608 - -0.6 635,608 - +0.4 635,608 - -0.8 635,608 - -1.1 635,608 - -0.2 635,608 - +0.2 635,608 - -1.8 635,608 - -1.0 635,608 - +0.3 635,918 + GFF2579 0.41 +0.8 635,918 + GFF2579 0.41 -1.5 635,918 + GFF2579 0.41 +0.1 635,918 + GFF2579 0.41 -0.8 635,918 + GFF2579 0.41 -1.2 635,918 + GFF2579 0.41 -0.5 635,918 + GFF2579 0.41 +0.6 635,918 + GFF2579 0.41 -0.2 635,918 + GFF2579 0.41 -0.1 635,919 - GFF2579 0.41 -1.3 635,919 - GFF2579 0.41 -0.7 635,919 - GFF2579 0.41 -0.5 635,919 - GFF2579 0.41 -0.0 635,919 - GFF2579 0.41 -0.5 635,919 - GFF2579 0.41 +0.5 635,919 - GFF2579 0.41 +0.2 635,919 - GFF2579 0.41 -1.8 635,919 - GFF2579 0.41 +0.2 635,919 - GFF2579 0.41 +0.1 635,919 - GFF2579 0.41 -1.9 635,919 - GFF2579 0.41 -0.2 635,919 - GFF2579 0.41 -1.6 636,084 + GFF2579 0.60 +1.2 636,084 + GFF2579 0.60 +1.7 636,085 - GFF2579 0.60 +0.9 636,165 + GFF2579 0.69 -0.5 636,165 + GFF2579 0.69 -1.1 636,165 + GFF2579 0.69 -0.1 636,166 - GFF2579 0.69 -2.0 636,291 + GFF2579 0.83 -0.3 636,291 + GFF2579 0.83 -0.2 636,292 - GFF2579 0.83 -0.9 636,292 - GFF2579 0.83 -1.2 636,292 - GFF2579 0.83 -0.8 636,399 + -0.4
Or see this region's nucleotide sequence