Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2254 and GFF2255 are separated by 8 nucleotides GFF2255 and GFF2256 are separated by 16 nucleotides GFF2256 and GFF2257 overlap by 8 nucleotides
GFF2254 - HYPOTHETICAL/UNKNOWN PROTEIN, at 309,807 to 310,448
GFF2254
GFF2255 - Ribonuclease Z (EC 3.1.26.11), at 310,457 to 311,413
GFF2255
GFF2256 - Uncharacterized protein YcgL, at 311,430 to 312,281
GFF2256
GFF2257 - RNA 3'-terminal phosphate cyclase (EC 6.5.1.4), at 312,274 to 313,311
GFF2257
Position (kb) 311
312
313 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 310.664 kb on + strand, within GFF2255 at 310.793 kb on + strand, within GFF2255 at 310.794 kb on - strand, within GFF2255 at 310.857 kb on + strand, within GFF2255 at 310.858 kb on - strand, within GFF2255 at 310.858 kb on - strand, within GFF2255 at 311.042 kb on + strand, within GFF2255 at 311.043 kb on - strand, within GFF2255 at 311.330 kb on + strand at 311.369 kb on + strand at 311.613 kb on + strand, within GFF2256 at 311.634 kb on + strand, within GFF2256 at 311.635 kb on - strand, within GFF2256 at 311.635 kb on - strand, within GFF2256 at 311.862 kb on + strand, within GFF2256 at 311.863 kb on - strand, within GFF2256 at 311.928 kb on + strand, within GFF2256 at 311.929 kb on - strand, within GFF2256 at 311.934 kb on + strand, within GFF2256 at 311.934 kb on + strand, within GFF2256 at 311.935 kb on - strand, within GFF2256 at 312.222 kb on + strand at 312.526 kb on + strand, within GFF2257 at 312.526 kb on + strand, within GFF2257 at 312.997 kb on + strand, within GFF2257 at 312.997 kb on + strand, within GFF2257
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 310,664 + GFF2255 0.22 -0.9 310,793 + GFF2255 0.35 +1.1 310,794 - GFF2255 0.35 +0.3 310,857 + GFF2255 0.42 +0.4 310,858 - GFF2255 0.42 +0.8 310,858 - GFF2255 0.42 -0.5 311,042 + GFF2255 0.61 +0.3 311,043 - GFF2255 0.61 -0.6 311,330 + -0.9 311,369 + +0.4 311,613 + GFF2256 0.21 +0.0 311,634 + GFF2256 0.24 -0.4 311,635 - GFF2256 0.24 +1.3 311,635 - GFF2256 0.24 -0.0 311,862 + GFF2256 0.51 -0.0 311,863 - GFF2256 0.51 -0.4 311,928 + GFF2256 0.58 -0.1 311,929 - GFF2256 0.59 -2.3 311,934 + GFF2256 0.59 +1.8 311,934 + GFF2256 0.59 -0.8 311,935 - GFF2256 0.59 +0.0 312,222 + -0.9 312,526 + GFF2257 0.24 +2.8 312,526 + GFF2257 0.24 +0.1 312,997 + GFF2257 0.70 -0.3 312,997 + GFF2257 0.70 +0.3
Or see this region's nucleotide sequence