Strain Fitness in Variovorax sp. SCN45 around GFF2130

Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2128 and GFF2129 are separated by 96 nucleotidesGFF2129 and GFF2130 are separated by 61 nucleotidesGFF2130 and GFF2131 overlap by 4 nucleotides GFF2128 - Enoyl-CoA hydratase (EC 4.2.1.17), at 177,643 to 178,422 GFF2128 GFF2129 - small multidrug resistance family (SMR) protein, at 178,519 to 178,854 GFF2129 GFF2130 - hypothetical protein, at 178,916 to 179,506 GFF2130 GFF2131 - Putative protease, at 179,503 to 181,353 GFF2131 Position (kb) 178 179 180Strain fitness (log2 ratio) -2 -1 0 1 2 3at 178.172 kb on + strand, within GFF2128at 178.172 kb on + strand, within GFF2128at 178.172 kb on + strand, within GFF2128at 178.172 kb on + strand, within GFF2128at 178.172 kb on + strand, within GFF2128at 178.172 kb on + strand, within GFF2128at 178.172 kb on + strand, within GFF2128at 178.184 kb on + strand, within GFF2128at 178.184 kb on + strand, within GFF2128at 178.184 kb on + strand, within GFF2128at 178.184 kb on + strand, within GFF2128at 178.184 kb on + strand, within GFF2128at 178.185 kb on - strand, within GFF2128at 178.185 kb on - strand, within GFF2128at 178.185 kb on - strand, within GFF2128at 178.185 kb on - strand, within GFF2128at 178.185 kb on - strand, within GFF2128at 178.202 kb on + strand, within GFF2128at 178.202 kb on + strand, within GFF2128at 178.202 kb on + strand, within GFF2128at 178.202 kb on + strand, within GFF2128at 178.202 kb on + strand, within GFF2128at 178.203 kb on - strand, within GFF2128at 178.203 kb on - strand, within GFF2128at 178.203 kb on - strand, within GFF2128at 178.203 kb on - strand, within GFF2128at 178.415 kb on + strandat 178.415 kb on + strandat 178.416 kb on - strandat 178.461 kb on - strandat 178.461 kb on - strandat 178.462 kb on + strandat 178.462 kb on + strandat 178.462 kb on + strandat 178.463 kb on - strandat 178.506 kb on + strandat 178.507 kb on - strandat 178.507 kb on - strandat 178.507 kb on - strandat 178.531 kb on + strandat 178.531 kb on + strandat 178.531 kb on + strandat 178.531 kb on + strandat 178.531 kb on + strandat 178.531 kb on + strandat 178.531 kb on + strandat 178.531 kb on + strandat 178.531 kb on + strandat 178.532 kb on - strandat 178.537 kb on + strandat 178.537 kb on + strandat 178.538 kb on - strandat 178.538 kb on - strandat 178.538 kb on - strandat 178.538 kb on - strandat 178.538 kb on - strandat 178.639 kb on + strand, within GFF2129at 178.640 kb on - strand, within GFF2129at 178.640 kb on - strand, within GFF2129at 178.699 kb on + strand, within GFF2129at 178.699 kb on + strand, within GFF2129at 178.946 kb on + strandat 178.946 kb on + strandat 178.946 kb on + strandat 178.947 kb on - strandat 178.947 kb on - strandat 178.947 kb on - strandat 178.947 kb on - strandat 178.947 kb on - strandat 179.282 kb on + strand, within GFF2130at 179.282 kb on + strand, within GFF2130at 179.283 kb on - strand, within GFF2130at 179.283 kb on - strand, within GFF2130at 179.283 kb on - strand, within GFF2130at 179.283 kb on - strand, within GFF2130at 179.283 kb on - strand, within GFF2130at 179.283 kb on - strand, within GFF2130at 179.396 kb on + strand, within GFF2130at 179.397 kb on - strand, within GFF2130at 179.504 kb on + strandat 179.505 kb on - strandat 179.648 kb on + strandat 179.648 kb on + strandat 179.648 kb on + strandat 179.649 kb on - strandat 179.649 kb on - strandat 179.954 kb on + strand, within GFF2131at 179.954 kb on + strand, within GFF2131at 179.955 kb on - strand, within GFF2131at 179.955 kb on - strand, within GFF2131at 179.955 kb on - strand, within GFF2131at 179.955 kb on - strand, within GFF2131at 180.119 kb on + strand, within GFF2131at 180.119 kb on + strand, within GFF2131at 180.119 kb on + strand, within GFF2131at 180.119 kb on + strand, within GFF2131at 180.120 kb on - strand, within GFF2131at 180.120 kb on - strand, within GFF2131at 180.120 kb on - strand, within GFF2131at 180.120 kb on - strand, within GFF2131at 180.120 kb on - strand, within GFF2131at 180.122 kb on + strand, within GFF2131at 180.122 kb on + strand, within GFF2131at 180.122 kb on + strand, within GFF2131at 180.122 kb on + strand, within GFF2131at 180.123 kb on - strand, within GFF2131at 180.123 kb on - strand, within GFF2131at 180.123 kb on - strand, within GFF2131at 180.123 kb on - strand, within GFF2131at 180.123 kb on - strand, within GFF2131at 180.123 kb on - strand, within GFF2131at 180.123 kb on - strand, within GFF2131at 180.123 kb on - strand, within GFF2131at 180.123 kb on - strand, within GFF2131at 180.123 kb on - strand, within GFF2131at 180.123 kb on - strand, within GFF2131at 180.123 kb on - strand, within GFF2131at 180.161 kb on + strand, within GFF2131at 180.161 kb on + strand, within GFF2131at 180.162 kb on - strand, within GFF2131at 180.162 kb on - strand, within GFF2131at 180.164 kb on + strand, within GFF2131at 180.164 kb on + strand, within GFF2131at 180.164 kb on + strand, within GFF2131at 180.164 kb on + strand, within GFF2131at 180.164 kb on + strand, within GFF2131at 180.164 kb on + strand, within GFF2131at 180.164 kb on + strand, within GFF2131at 180.165 kb on - strand, within GFF2131at 180.165 kb on - strand, within GFF2131at 180.263 kb on + strand, within GFF2131at 180.311 kb on + strand, within GFF2131at 180.312 kb on - strand, within GFF2131at 180.312 kb on - strand, within GFF2131at 180.312 kb on - strand, within GFF2131at 180.312 kb on - strand, within GFF2131at 180.314 kb on + strand, within GFF2131at 180.314 kb on + strand, within GFF2131at 180.314 kb on + strand, within GFF2131at 180.315 kb on - strand, within GFF2131at 180.315 kb on - strand, within GFF2131at 180.315 kb on - strand, within GFF2131at 180.315 kb on - strand, within GFF2131at 180.315 kb on - strand, within GFF2131at 180.353 kb on + strand, within GFF2131at 180.353 kb on + strand, within GFF2131at 180.353 kb on + strand, within GFF2131at 180.368 kb on + strand, within GFF2131at 180.368 kb on + strand, within GFF2131at 180.369 kb on - strand, within GFF2131at 180.369 kb on - strand, within GFF2131at 180.369 kb on - strand, within GFF2131at 180.369 kb on - strand, within GFF2131at 180.369 kb on - strand, within GFF2131at 180.425 kb on + strand, within GFF2131at 180.425 kb on + strand, within GFF2131at 180.425 kb on + strand, within GFF2131at 180.425 kb on + strand, within GFF2131at 180.425 kb on + strand, within GFF2131at 180.425 kb on + strand, within GFF2131at 180.425 kb on + strand, within GFF2131at 180.426 kb on - strand, within GFF2131at 180.426 kb on - strand, within GFF2131at 180.458 kb on + strand, within GFF2131at 180.458 kb on + strand, within GFF2131at 180.458 kb on + strand, within GFF2131at 180.458 kb on + strand, within GFF2131at 180.459 kb on - strand, within GFF2131at 180.459 kb on - strand, within GFF2131at 180.459 kb on - strand, within GFF2131at 180.459 kb on - strand, within GFF2131at 180.459 kb on - strand, within GFF2131

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
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178,172 + GFF2128 0.68 +0.2
178,172 + GFF2128 0.68 -1.3
178,172 + GFF2128 0.68 +1.5
178,172 + GFF2128 0.68 -0.6
178,172 + GFF2128 0.68 +0.1
178,172 + GFF2128 0.68 -0.2
178,172 + GFF2128 0.68 +0.6
178,184 + GFF2128 0.69 -0.2
178,184 + GFF2128 0.69 -1.0
178,184 + GFF2128 0.69 -0.3
178,184 + GFF2128 0.69 +0.3
178,184 + GFF2128 0.69 +0.3
178,185 - GFF2128 0.69 +0.2
178,185 - GFF2128 0.69 -0.5
178,185 - GFF2128 0.69 -1.4
178,185 - GFF2128 0.69 -0.1
178,185 - GFF2128 0.69 -2.2
178,202 + GFF2128 0.72 -0.9
178,202 + GFF2128 0.72 -0.8
178,202 + GFF2128 0.72 +0.9
178,202 + GFF2128 0.72 -0.3
178,202 + GFF2128 0.72 -1.0
178,203 - GFF2128 0.72 -0.5
178,203 - GFF2128 0.72 +0.4
178,203 - GFF2128 0.72 -0.2
178,203 - GFF2128 0.72 -0.9
178,415 + +1.5
178,415 + +0.6
178,416 - +0.1
178,461 - -1.8
178,461 - -0.8
178,462 + -0.3
178,462 + -0.1
178,462 + -0.1
178,463 - +1.2
178,506 + +0.3
178,507 - -0.3
178,507 - -0.6
178,507 - +0.1
178,531 + -0.4
178,531 + +0.1
178,531 + -0.7
178,531 + -0.4
178,531 + +0.9
178,531 + -0.7
178,531 + -0.1
178,531 + -0.6
178,531 + -1.6
178,532 - -0.9
178,537 + -0.6
178,537 + -0.1
178,538 - -0.3
178,538 - -0.8
178,538 - +0.6
178,538 - -0.0
178,538 - +0.3
178,639 + GFF2129 0.36 +0.5
178,640 - GFF2129 0.36 -0.7
178,640 - GFF2129 0.36 -0.5
178,699 + GFF2129 0.54 +0.5
178,699 + GFF2129 0.54 -0.6
178,946 + -1.1
178,946 + -0.7
178,946 + +1.2
178,947 - -0.4
178,947 - +1.1
178,947 - +0.1
178,947 - -0.5
178,947 - +0.3
179,282 + GFF2130 0.62 +0.3
179,282 + GFF2130 0.62 +1.2
179,283 - GFF2130 0.62 -1.1
179,283 - GFF2130 0.62 +1.3
179,283 - GFF2130 0.62 +1.5
179,283 - GFF2130 0.62 +0.1
179,283 - GFF2130 0.62 +0.1
179,283 - GFF2130 0.62 +0.3
179,396 + GFF2130 0.81 -0.5
179,397 - GFF2130 0.81 -0.9
179,504 + -0.3
179,505 - -1.5
179,648 + +0.3
179,648 + -0.5
179,648 + +1.0
179,649 - -0.4
179,649 - -0.5
179,954 + GFF2131 0.24 +1.3
179,954 + GFF2131 0.24 +0.1
179,955 - GFF2131 0.24 +0.5
179,955 - GFF2131 0.24 -1.6
179,955 - GFF2131 0.24 +0.2
179,955 - GFF2131 0.24 -0.0
180,119 + GFF2131 0.33 -1.4
180,119 + GFF2131 0.33 +1.1
180,119 + GFF2131 0.33 -0.5
180,119 + GFF2131 0.33 -2.0
180,120 - GFF2131 0.33 -0.6
180,120 - GFF2131 0.33 -0.8
180,120 - GFF2131 0.33 +0.1
180,120 - GFF2131 0.33 +0.3
180,120 - GFF2131 0.33 -0.7
180,122 + GFF2131 0.33 -0.9
180,122 + GFF2131 0.33 +0.3
180,122 + GFF2131 0.33 +0.2
180,122 + GFF2131 0.33 -0.4
180,123 - GFF2131 0.33 +0.5
180,123 - GFF2131 0.33 +0.5
180,123 - GFF2131 0.33 +0.1
180,123 - GFF2131 0.33 -1.0
180,123 - GFF2131 0.33 +0.2
180,123 - GFF2131 0.33 -1.0
180,123 - GFF2131 0.33 +0.3
180,123 - GFF2131 0.33 -0.9
180,123 - GFF2131 0.33 +3.1
180,123 - GFF2131 0.33 -1.2
180,123 - GFF2131 0.33 -1.2
180,123 - GFF2131 0.33 -1.3
180,161 + GFF2131 0.36 -0.3
180,161 + GFF2131 0.36 +0.7
180,162 - GFF2131 0.36 -0.1
180,162 - GFF2131 0.36 -1.4
180,164 + GFF2131 0.36 -1.0
180,164 + GFF2131 0.36 -2.6
180,164 + GFF2131 0.36 -0.4
180,164 + GFF2131 0.36 +0.3
180,164 + GFF2131 0.36 -0.0
180,164 + GFF2131 0.36 -0.4
180,164 + GFF2131 0.36 +0.9
180,165 - GFF2131 0.36 +0.3
180,165 - GFF2131 0.36 -1.4
180,263 + GFF2131 0.41 +0.2
180,311 + GFF2131 0.44 -0.1
180,312 - GFF2131 0.44 -1.7
180,312 - GFF2131 0.44 -0.0
180,312 - GFF2131 0.44 -0.4
180,312 - GFF2131 0.44 -0.5
180,314 + GFF2131 0.44 +0.5
180,314 + GFF2131 0.44 +1.5
180,314 + GFF2131 0.44 -0.4
180,315 - GFF2131 0.44 +0.2
180,315 - GFF2131 0.44 -0.6
180,315 - GFF2131 0.44 -1.0
180,315 - GFF2131 0.44 +0.1
180,315 - GFF2131 0.44 -0.8
180,353 + GFF2131 0.46 -0.1
180,353 + GFF2131 0.46 +0.5
180,353 + GFF2131 0.46 +0.3
180,368 + GFF2131 0.47 +0.2
180,368 + GFF2131 0.47 -0.0
180,369 - GFF2131 0.47 +1.0
180,369 - GFF2131 0.47 -0.7
180,369 - GFF2131 0.47 +0.4
180,369 - GFF2131 0.47 -0.3
180,369 - GFF2131 0.47 +0.4
180,425 + GFF2131 0.50 -0.1
180,425 + GFF2131 0.50 -1.5
180,425 + GFF2131 0.50 +2.9
180,425 + GFF2131 0.50 -0.6
180,425 + GFF2131 0.50 +0.9
180,425 + GFF2131 0.50 -0.9
180,425 + GFF2131 0.50 -1.0
180,426 - GFF2131 0.50 -0.5
180,426 - GFF2131 0.50 -0.7
180,458 + GFF2131 0.52 +1.0
180,458 + GFF2131 0.52 -1.7
180,458 + GFF2131 0.52 +0.3
180,458 + GFF2131 0.52 +0.3
180,459 - GFF2131 0.52 +0.2
180,459 - GFF2131 0.52 +1.7
180,459 - GFF2131 0.52 +0.4
180,459 - GFF2131 0.52 -0.7
180,459 - GFF2131 0.52 +0.2

Or see this region's nucleotide sequence