Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1983 and GFF1984 overlap by 1 nucleotides GFF1984 and GFF1985 are separated by 17 nucleotides GFF1985 and GFF1986 are separated by 21 nucleotides GFF1986 and GFF1987 are separated by 68 nucleotides
GFF1983 - D-glycero-beta-D-manno-heptose-1,7-bisphosphate 7-phosphatase (EC 3.1.3.82); Histidinol-phosphatase (EC 3.1.3.15), at 25,408 to 25,986
GFF1983
GFF1984 - Phospholipid and glycerol acyltransferase (EC 2.3.1.51), at 25,986 to 26,756
GFF1984
GFF1985 - Zinc metalloprotease, at 26,774 to 27,754
GFF1985
GFF1986 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 27,776 to 28,546
GFF1986
GFF1987 - Pseudouridine synthase (EC 4.2.1.70), PA2043 type, at 28,615 to 29,535
GFF1987
Position (kb) 26
27
28 Strain fitness (log2 ratio) -2
-1
0
1 at 26.283 kb on + strand, within GFF1984 at 26.284 kb on - strand, within GFF1984 at 26.307 kb on + strand, within GFF1984 at 26.307 kb on + strand, within GFF1984 at 26.307 kb on + strand, within GFF1984 at 26.308 kb on - strand, within GFF1984 at 26.308 kb on - strand, within GFF1984 at 26.484 kb on + strand, within GFF1984 at 26.713 kb on - strand at 26.900 kb on - strand, within GFF1985 at 27.056 kb on + strand, within GFF1985 at 27.614 kb on + strand, within GFF1985 at 27.614 kb on + strand, within GFF1985 at 27.615 kb on - strand, within GFF1985 at 27.615 kb on - strand, within GFF1985 at 27.701 kb on + strand at 27.701 kb on + strand at 27.828 kb on + strand at 27.828 kb on + strand at 27.829 kb on - strand at 27.829 kb on - strand at 27.829 kb on - strand at 27.829 kb on - strand at 27.852 kb on + strand at 27.852 kb on + strand at 27.852 kb on + strand at 27.852 kb on + strand at 27.852 kb on + strand at 27.852 kb on + strand at 27.852 kb on + strand at 27.852 kb on + strand at 27.853 kb on - strand, within GFF1986 at 27.853 kb on - strand, within GFF1986 at 27.853 kb on - strand, within GFF1986 at 28.059 kb on + strand, within GFF1986 at 28.065 kb on + strand, within GFF1986 at 28.066 kb on - strand, within GFF1986 at 28.066 kb on - strand, within GFF1986 at 28.066 kb on - strand, within GFF1986 at 28.305 kb on + strand, within GFF1986 at 28.306 kb on - strand, within GFF1986 at 28.431 kb on + strand, within GFF1986 at 28.432 kb on - strand, within GFF1986 at 28.432 kb on - strand, within GFF1986 at 28.432 kb on - strand, within GFF1986 at 28.590 kb on + strand at 28.590 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 26,283 + GFF1984 0.39 -2.1 26,284 - GFF1984 0.39 -0.5 26,307 + GFF1984 0.42 -0.9 26,307 + GFF1984 0.42 -1.3 26,307 + GFF1984 0.42 -0.8 26,308 - GFF1984 0.42 -0.4 26,308 - GFF1984 0.42 +1.5 26,484 + GFF1984 0.65 -0.4 26,713 - -1.9 26,900 - GFF1985 0.13 -0.8 27,056 + GFF1985 0.29 -0.6 27,614 + GFF1985 0.86 +0.6 27,614 + GFF1985 0.86 +0.1 27,615 - GFF1985 0.86 +1.5 27,615 - GFF1985 0.86 -0.9 27,701 + +0.4 27,701 + -0.8 27,828 + -0.4 27,828 + -0.2 27,829 - -1.6 27,829 - -0.5 27,829 - +0.9 27,829 - +0.5 27,852 + +0.2 27,852 + +0.0 27,852 + +0.5 27,852 + +0.1 27,852 + -0.3 27,852 + -0.4 27,852 + +0.2 27,852 + -0.3 27,853 - GFF1986 0.10 -0.9 27,853 - GFF1986 0.10 -0.6 27,853 - GFF1986 0.10 -0.2 28,059 + GFF1986 0.37 +0.7 28,065 + GFF1986 0.37 +0.5 28,066 - GFF1986 0.38 -0.6 28,066 - GFF1986 0.38 +1.0 28,066 - GFF1986 0.38 -0.8 28,305 + GFF1986 0.69 -1.5 28,306 - GFF1986 0.69 -1.2 28,431 + GFF1986 0.85 -0.9 28,432 - GFF1986 0.85 -0.6 28,432 - GFF1986 0.85 -0.6 28,432 - GFF1986 0.85 -0.6 28,590 + -0.5 28,590 + -0.5
Or see this region's nucleotide sequence