Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1828 and GFF1829 are separated by 112 nucleotides GFF1829 and GFF1830 overlap by 1 nucleotides GFF1830 and GFF1831 overlap by 4 nucleotides GFF1831 and GFF1832 are separated by 80 nucleotides
GFF1828 - phosphodiesterase I, at 493,174 to 495,015
GFF1828
GFF1829 - 4-hydroxybenzoyl-CoA thioesterase (EC 3.1.2.23), at 495,128 to 495,556
GFF1829
GFF1830 - TRAP dicarboxylate transporter, DctM subunit, unknown substrate 3, at 495,556 to 496,857
GFF1830
GFF1831 - TRAP dicarboxylate transporter, DctQ subunit, unknown substrate 3, at 496,854 to 497,354
GFF1831
GFF1832 - TRAP transporter solute receptor, unknown substrate 3, at 497,435 to 498,490
GFF1832
Position (kb) 495
496
497 Strain fitness (log2 ratio) -2
-1
0
1
2 at 494.847 kb on - strand at 494.904 kb on + strand at 494.905 kb on - strand at 494.979 kb on - strand at 494.979 kb on - strand at 495.256 kb on - strand, within GFF1829 at 495.322 kb on - strand, within GFF1829 at 495.869 kb on + strand, within GFF1830 at 496.286 kb on + strand, within GFF1830 at 496.745 kb on + strand at 496.751 kb on + strand at 496.806 kb on + strand at 496.810 kb on + strand at 496.811 kb on - strand at 496.811 kb on - strand at 496.811 kb on - strand at 496.975 kb on + strand, within GFF1831 at 496.975 kb on + strand, within GFF1831 at 496.976 kb on - strand, within GFF1831 at 496.976 kb on - strand, within GFF1831 at 497.005 kb on + strand, within GFF1831 at 497.173 kb on + strand, within GFF1831 at 497.357 kb on - strand at 497.451 kb on + strand at 497.589 kb on + strand, within GFF1832 at 497.590 kb on - strand, within GFF1832 at 497.730 kb on + strand, within GFF1832 at 497.731 kb on - strand, within GFF1832 at 497.731 kb on - strand, within GFF1832 at 497.731 kb on - strand, within GFF1832 at 497.769 kb on + strand, within GFF1832 at 497.769 kb on + strand, within GFF1832 at 497.770 kb on - strand, within GFF1832 at 497.770 kb on - strand, within GFF1832 at 497.791 kb on - strand, within GFF1832
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 494,847 - -0.8 494,904 + -0.7 494,905 - -0.7 494,979 - +0.4 494,979 - -0.4 495,256 - GFF1829 0.30 +1.5 495,322 - GFF1829 0.45 -0.1 495,869 + GFF1830 0.24 -0.9 496,286 + GFF1830 0.56 -1.0 496,745 + -0.1 496,751 + +1.3 496,806 + -0.3 496,810 + +0.4 496,811 - +0.7 496,811 - -1.2 496,811 - -0.2 496,975 + GFF1831 0.24 -1.0 496,975 + GFF1831 0.24 -2.0 496,976 - GFF1831 0.24 -0.1 496,976 - GFF1831 0.24 +1.1 497,005 + GFF1831 0.30 +1.8 497,173 + GFF1831 0.64 +1.3 497,357 - -0.8 497,451 + +1.0 497,589 + GFF1832 0.15 +1.2 497,590 - GFF1832 0.15 +0.5 497,730 + GFF1832 0.28 +0.6 497,731 - GFF1832 0.28 +0.3 497,731 - GFF1832 0.28 +0.1 497,731 - GFF1832 0.28 +0.1 497,769 + GFF1832 0.32 -1.2 497,769 + GFF1832 0.32 +0.5 497,770 - GFF1832 0.32 -0.4 497,770 - GFF1832 0.32 +0.7 497,791 - GFF1832 0.34 -0.7
Or see this region's nucleotide sequence