Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1688 and GFF1689 are separated by 39 nucleotides GFF1689 and GFF1690 are separated by 21 nucleotides GFF1690 and GFF1691 are separated by 101 nucleotides
GFF1688 - FIG00828468: hypothetical protein, at 332,813 to 334,240
GFF1688
GFF1689 - BUG/TctC family periplasmic protein, at 334,280 to 335,272
GFF1689
GFF1690 - KEGG: DNA-binding protein H-NS, at 335,294 to 335,647
GFF1690
GFF1691 - Two component, Sigma-54 Specific, central transcriptional regulator of acidic amino acid uptake, at 335,749 to 337,110
GFF1691
Position (kb)
334
335
336 Strain fitness (log2 ratio)
-2
-1
0
1 at 333.434 kb on + strand, within GFF1688 at 333.488 kb on + strand, within GFF1688 at 333.489 kb on - strand, within GFF1688 at 333.515 kb on + strand, within GFF1688 at 333.515 kb on + strand, within GFF1688 at 333.656 kb on + strand, within GFF1688 at 333.774 kb on - strand, within GFF1688 at 333.839 kb on + strand, within GFF1688 at 333.839 kb on + strand, within GFF1688 at 333.839 kb on + strand, within GFF1688 at 334.101 kb on - strand at 334.101 kb on - strand at 334.538 kb on + strand, within GFF1689 at 334.565 kb on + strand, within GFF1689 at 334.567 kb on + strand, within GFF1689 at 334.567 kb on + strand, within GFF1689 at 334.567 kb on + strand, within GFF1689 at 334.567 kb on + strand, within GFF1689 at 334.567 kb on + strand, within GFF1689 at 334.567 kb on + strand, within GFF1689 at 334.567 kb on + strand, within GFF1689 at 334.567 kb on + strand, within GFF1689 at 334.567 kb on + strand, within GFF1689 at 334.567 kb on + strand, within GFF1689 at 334.567 kb on + strand, within GFF1689 at 334.567 kb on + strand, within GFF1689 at 334.567 kb on + strand, within GFF1689 at 334.567 kb on + strand, within GFF1689 at 334.567 kb on + strand, within GFF1689 at 334.567 kb on + strand, within GFF1689 at 334.567 kb on + strand, within GFF1689 at 334.567 kb on + strand, within GFF1689 at 334.567 kb on + strand, within GFF1689 at 334.567 kb on + strand, within GFF1689 at 334.567 kb on + strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.568 kb on - strand, within GFF1689 at 334.628 kb on + strand, within GFF1689 at 334.828 kb on - strand, within GFF1689 at 334.847 kb on + strand, within GFF1689 at 334.847 kb on + strand, within GFF1689 at 334.847 kb on + strand, within GFF1689 at 334.848 kb on - strand, within GFF1689 at 334.848 kb on - strand, within GFF1689 at 334.848 kb on - strand, within GFF1689 at 334.848 kb on - strand, within GFF1689 at 334.848 kb on - strand, within GFF1689 at 334.848 kb on - strand, within GFF1689 at 335.271 kb on - strand at 335.271 kb on - strand at 335.295 kb on + strand at 335.296 kb on - strand at 335.523 kb on + strand, within GFF1690 at 335.533 kb on + strand, within GFF1690 at 335.534 kb on - strand, within GFF1690 at 335.631 kb on + strand at 335.631 kb on + strand at 335.671 kb on - strand at 335.686 kb on + strand at 335.686 kb on + strand at 335.686 kb on + strand at 335.686 kb on + strand at 335.686 kb on + strand at 335.686 kb on + strand at 335.686 kb on + strand at 335.686 kb on + strand at 335.686 kb on + strand at 335.686 kb on + strand at 335.686 kb on + strand at 335.687 kb on - strand at 335.687 kb on - strand at 335.687 kb on - strand at 335.687 kb on - strand at 335.687 kb on - strand at 335.687 kb on - strand at 335.687 kb on - strand at 335.750 kb on + strand at 336.035 kb on + strand, within GFF1691 at 336.035 kb on + strand, within GFF1691 at 336.035 kb on + strand, within GFF1691 at 336.035 kb on + strand, within GFF1691 at 336.035 kb on + strand, within GFF1691 at 336.036 kb on - strand, within GFF1691 at 336.036 kb on - strand, within GFF1691 at 336.036 kb on - strand, within GFF1691 at 336.036 kb on - strand, within GFF1691 at 336.090 kb on - strand, within GFF1691 at 336.191 kb on + strand, within GFF1691 at 336.192 kb on - strand, within GFF1691 at 336.194 kb on + strand, within GFF1691 at 336.194 kb on + strand, within GFF1691 at 336.194 kb on + strand, within GFF1691 at 336.194 kb on + strand, within GFF1691 at 336.194 kb on + strand, within GFF1691 at 336.195 kb on - strand, within GFF1691 at 336.195 kb on - strand, within GFF1691 at 336.195 kb on - strand, within GFF1691 at 336.195 kb on - strand, within GFF1691 at 336.195 kb on - strand, within GFF1691
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 333,434 + GFF1688 0.43 +0.7 333,488 + GFF1688 0.47 +0.1 333,489 - GFF1688 0.47 +1.0 333,515 + GFF1688 0.49 -0.8 333,515 + GFF1688 0.49 -0.2 333,656 + GFF1688 0.59 +0.5 333,774 - GFF1688 0.67 +0.0 333,839 + GFF1688 0.72 -1.8 333,839 + GFF1688 0.72 +0.4 333,839 + GFF1688 0.72 +0.3 334,101 - +0.5 334,101 - -1.8 334,538 + GFF1689 0.26 +0.9 334,565 + GFF1689 0.29 -1.0 334,567 + GFF1689 0.29 +1.6 334,567 + GFF1689 0.29 +1.0 334,567 + GFF1689 0.29 +1.6 334,567 + GFF1689 0.29 +0.6 334,567 + GFF1689 0.29 -0.3 334,567 + GFF1689 0.29 -0.4 334,567 + GFF1689 0.29 +0.4 334,567 + GFF1689 0.29 +1.1 334,567 + GFF1689 0.29 -2.1 334,567 + GFF1689 0.29 -0.1 334,567 + GFF1689 0.29 -1.6 334,567 + GFF1689 0.29 +0.8 334,567 + GFF1689 0.29 -0.5 334,567 + GFF1689 0.29 -0.3 334,567 + GFF1689 0.29 -0.3 334,567 + GFF1689 0.29 -0.0 334,567 + GFF1689 0.29 -0.7 334,567 + GFF1689 0.29 -1.3 334,567 + GFF1689 0.29 +0.3 334,567 + GFF1689 0.29 -0.3 334,567 + GFF1689 0.29 +0.3 334,568 - GFF1689 0.29 +1.0 334,568 - GFF1689 0.29 -0.3 334,568 - GFF1689 0.29 +0.3 334,568 - GFF1689 0.29 +0.3 334,568 - GFF1689 0.29 -0.6 334,568 - GFF1689 0.29 +1.2 334,568 - GFF1689 0.29 -0.8 334,568 - GFF1689 0.29 +1.1 334,568 - GFF1689 0.29 +0.5 334,568 - GFF1689 0.29 +0.3 334,568 - GFF1689 0.29 -1.6 334,568 - GFF1689 0.29 -0.3 334,568 - GFF1689 0.29 +0.9 334,568 - GFF1689 0.29 +0.5 334,568 - GFF1689 0.29 -1.2 334,568 - GFF1689 0.29 -0.5 334,568 - GFF1689 0.29 -0.3 334,568 - GFF1689 0.29 -0.1 334,568 - GFF1689 0.29 +0.0 334,568 - GFF1689 0.29 -0.3 334,568 - GFF1689 0.29 -2.0 334,568 - GFF1689 0.29 +0.7 334,568 - GFF1689 0.29 +0.1 334,568 - GFF1689 0.29 +1.2 334,628 + GFF1689 0.35 +0.2 334,828 - GFF1689 0.55 -0.6 334,847 + GFF1689 0.57 -0.3 334,847 + GFF1689 0.57 +0.0 334,847 + GFF1689 0.57 +0.6 334,848 - GFF1689 0.57 +0.4 334,848 - GFF1689 0.57 +0.9 334,848 - GFF1689 0.57 +0.6 334,848 - GFF1689 0.57 +0.3 334,848 - GFF1689 0.57 +0.7 334,848 - GFF1689 0.57 -0.1 335,271 - -0.4 335,271 - -0.2 335,295 + -2.1 335,296 - -2.6 335,523 + GFF1690 0.65 -0.1 335,533 + GFF1690 0.68 -0.2 335,534 - GFF1690 0.68 +0.8 335,631 + +1.1 335,631 + -1.2 335,671 - +0.3 335,686 + -0.7 335,686 + +0.0 335,686 + -0.2 335,686 + +0.3 335,686 + +0.0 335,686 + +0.1 335,686 + -0.7 335,686 + +0.8 335,686 + +0.8 335,686 + -1.3 335,686 + -0.4 335,687 - +0.0 335,687 - +0.3 335,687 - -1.0 335,687 - -1.5 335,687 - -0.4 335,687 - +0.5 335,687 - +0.8 335,750 + -0.3 336,035 + GFF1691 0.21 -2.0 336,035 + GFF1691 0.21 +0.8 336,035 + GFF1691 0.21 +0.1 336,035 + GFF1691 0.21 -0.0 336,035 + GFF1691 0.21 -0.2 336,036 - GFF1691 0.21 -0.0 336,036 - GFF1691 0.21 -1.2 336,036 - GFF1691 0.21 +0.4 336,036 - GFF1691 0.21 -0.1 336,090 - GFF1691 0.25 +0.3 336,191 + GFF1691 0.32 -0.2 336,192 - GFF1691 0.33 -0.7 336,194 + GFF1691 0.33 +0.8 336,194 + GFF1691 0.33 +1.0 336,194 + GFF1691 0.33 -0.3 336,194 + GFF1691 0.33 +1.3 336,194 + GFF1691 0.33 +0.2 336,195 - GFF1691 0.33 -1.5 336,195 - GFF1691 0.33 -1.0 336,195 - GFF1691 0.33 +0.0 336,195 - GFF1691 0.33 -1.7 336,195 - GFF1691 0.33 -2.7
Or see this region's nucleotide sequence