Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1667 and GFF1668 are separated by 3 nucleotides GFF1668 and GFF1669 are separated by 31 nucleotides
GFF1667 - N-methylhydantoinase B (EC 3.5.2.14), at 312,046 to 313,728
GFF1667
GFF1668 - BUG/TctC family periplasmic protein, at 313,732 to 314,700
GFF1668
GFF1669 - hypothetical protein, at 314,732 to 316,075
GFF1669
Position (kb) 313
314
315 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 312.839 kb on + strand, within GFF1667 at 312.839 kb on + strand, within GFF1667 at 312.840 kb on - strand, within GFF1667 at 312.840 kb on - strand, within GFF1667 at 312.840 kb on - strand, within GFF1667 at 312.840 kb on - strand, within GFF1667 at 312.840 kb on - strand, within GFF1667 at 313.007 kb on + strand, within GFF1667 at 313.103 kb on + strand, within GFF1667 at 313.307 kb on + strand, within GFF1667 at 313.307 kb on + strand, within GFF1667 at 313.308 kb on - strand, within GFF1667 at 313.308 kb on - strand, within GFF1667 at 313.361 kb on + strand, within GFF1667 at 313.361 kb on + strand, within GFF1667 at 313.367 kb on + strand, within GFF1667 at 313.367 kb on + strand, within GFF1667 at 313.367 kb on + strand, within GFF1667 at 313.433 kb on + strand, within GFF1667 at 313.976 kb on + strand, within GFF1668 at 313.977 kb on - strand, within GFF1668 at 314.391 kb on - strand, within GFF1668 at 314.854 kb on - strand at 314.854 kb on - strand at 314.854 kb on - strand at 315.168 kb on + strand, within GFF1669 at 315.186 kb on + strand, within GFF1669 at 315.186 kb on + strand, within GFF1669 at 315.267 kb on + strand, within GFF1669 at 315.268 kb on - strand, within GFF1669 at 315.375 kb on + strand, within GFF1669 at 315.376 kb on - strand, within GFF1669 at 315.462 kb on + strand, within GFF1669 at 315.462 kb on + strand, within GFF1669 at 315.463 kb on - strand, within GFF1669 at 315.463 kb on - strand, within GFF1669 at 315.501 kb on + strand, within GFF1669 at 315.502 kb on - strand, within GFF1669
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 312,839 + GFF1667 0.47 +1.3 312,839 + GFF1667 0.47 -0.9 312,840 - GFF1667 0.47 -0.0 312,840 - GFF1667 0.47 +0.6 312,840 - GFF1667 0.47 -0.8 312,840 - GFF1667 0.47 -1.1 312,840 - GFF1667 0.47 -0.3 313,007 + GFF1667 0.57 +0.3 313,103 + GFF1667 0.63 -0.1 313,307 + GFF1667 0.75 -0.3 313,307 + GFF1667 0.75 +0.3 313,308 - GFF1667 0.75 -0.0 313,308 - GFF1667 0.75 +2.9 313,361 + GFF1667 0.78 -0.8 313,361 + GFF1667 0.78 -0.6 313,367 + GFF1667 0.78 -0.6 313,367 + GFF1667 0.78 +0.9 313,367 + GFF1667 0.78 -0.7 313,433 + GFF1667 0.82 -1.3 313,976 + GFF1668 0.25 -0.8 313,977 - GFF1668 0.25 -0.3 314,391 - GFF1668 0.68 -1.0 314,854 - -0.1 314,854 - -1.5 314,854 - -0.2 315,168 + GFF1669 0.32 +0.0 315,186 + GFF1669 0.34 -0.7 315,186 + GFF1669 0.34 -0.5 315,267 + GFF1669 0.40 +0.0 315,268 - GFF1669 0.40 +0.9 315,375 + GFF1669 0.48 +1.1 315,376 - GFF1669 0.48 -2.7 315,462 + GFF1669 0.54 -0.1 315,462 + GFF1669 0.54 +1.3 315,463 - GFF1669 0.54 +1.5 315,463 - GFF1669 0.54 +0.6 315,501 + GFF1669 0.57 -0.5 315,502 - GFF1669 0.57 -0.7
Or see this region's nucleotide sequence