Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1598 and GFF1599 overlap by 4 nucleotides GFF1599 and GFF1600 overlap by 4 nucleotides GFF1600 and GFF1601 are separated by 19 nucleotides
GFF1598 - no description, at 196,750 to 197,148
GFF1598
GFF1599 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 197,145 to 198,116
GFF1599
GFF1600 - Branched-chain amino acid ABC transporter, permease protein LivH (TC 3.A.1.4.1), at 198,113 to 199,000
GFF1600
GFF1601 - no description, at 199,020 to 200,219
GFF1601
Position (kb) 198
199
200 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 197.431 kb on + strand, within GFF1599 at 197.461 kb on + strand, within GFF1599 at 197.461 kb on + strand, within GFF1599 at 197.461 kb on + strand, within GFF1599 at 197.462 kb on - strand, within GFF1599 at 197.462 kb on - strand, within GFF1599 at 197.500 kb on + strand, within GFF1599 at 197.518 kb on + strand, within GFF1599 at 197.518 kb on + strand, within GFF1599 at 197.518 kb on + strand, within GFF1599 at 197.518 kb on + strand, within GFF1599 at 197.519 kb on - strand, within GFF1599 at 197.519 kb on - strand, within GFF1599 at 197.519 kb on - strand, within GFF1599 at 197.519 kb on - strand, within GFF1599 at 197.519 kb on - strand, within GFF1599 at 197.519 kb on - strand, within GFF1599 at 197.519 kb on - strand, within GFF1599 at 197.717 kb on - strand, within GFF1599 at 197.860 kb on + strand, within GFF1599 at 197.887 kb on + strand, within GFF1599 at 197.983 kb on + strand, within GFF1599 at 197.984 kb on - strand, within GFF1599 at 197.984 kb on - strand, within GFF1599 at 197.999 kb on - strand, within GFF1599 at 197.999 kb on - strand, within GFF1599 at 197.999 kb on - strand, within GFF1599 at 197.999 kb on - strand, within GFF1599 at 197.999 kb on - strand, within GFF1599 at 198.073 kb on + strand at 198.073 kb on + strand at 198.074 kb on - strand at 198.074 kb on - strand at 198.074 kb on - strand at 198.106 kb on + strand at 198.106 kb on + strand at 198.107 kb on - strand at 198.107 kb on - strand at 198.107 kb on - strand at 198.204 kb on + strand, within GFF1600 at 198.715 kb on + strand, within GFF1600 at 198.715 kb on + strand, within GFF1600 at 199.066 kb on + strand at 199.303 kb on + strand, within GFF1601 at 199.303 kb on + strand, within GFF1601 at 199.318 kb on + strand, within GFF1601 at 199.318 kb on + strand, within GFF1601 at 199.319 kb on - strand, within GFF1601 at 199.319 kb on - strand, within GFF1601 at 199.363 kb on + strand, within GFF1601 at 199.363 kb on + strand, within GFF1601 at 199.364 kb on - strand, within GFF1601 at 199.364 kb on - strand, within GFF1601 at 199.403 kb on - strand, within GFF1601 at 199.487 kb on - strand, within GFF1601 at 199.499 kb on - strand, within GFF1601 at 199.512 kb on + strand, within GFF1601 at 199.534 kb on + strand, within GFF1601 at 199.535 kb on - strand, within GFF1601 at 199.615 kb on + strand, within GFF1601 at 199.675 kb on + strand, within GFF1601 at 199.675 kb on + strand, within GFF1601 at 199.676 kb on - strand, within GFF1601 at 199.795 kb on + strand, within GFF1601 at 199.796 kb on - strand, within GFF1601 at 199.903 kb on + strand, within GFF1601 at 199.904 kb on - strand, within GFF1601 at 199.904 kb on - strand, within GFF1601 at 199.904 kb on - strand, within GFF1601
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 197,431 + GFF1599 0.29 +0.5 197,461 + GFF1599 0.33 -0.5 197,461 + GFF1599 0.33 +0.4 197,461 + GFF1599 0.33 +0.6 197,462 - GFF1599 0.33 +2.8 197,462 - GFF1599 0.33 +0.1 197,500 + GFF1599 0.37 -0.8 197,518 + GFF1599 0.38 +0.0 197,518 + GFF1599 0.38 -0.1 197,518 + GFF1599 0.38 +0.2 197,518 + GFF1599 0.38 -1.8 197,519 - GFF1599 0.38 -2.6 197,519 - GFF1599 0.38 -0.4 197,519 - GFF1599 0.38 -0.3 197,519 - GFF1599 0.38 -0.1 197,519 - GFF1599 0.38 -0.3 197,519 - GFF1599 0.38 -0.2 197,519 - GFF1599 0.38 +0.6 197,717 - GFF1599 0.59 +1.2 197,860 + GFF1599 0.74 -0.2 197,887 + GFF1599 0.76 -0.1 197,983 + GFF1599 0.86 -1.2 197,984 - GFF1599 0.86 -0.4 197,984 - GFF1599 0.86 -1.6 197,999 - GFF1599 0.88 -0.7 197,999 - GFF1599 0.88 -1.2 197,999 - GFF1599 0.88 +0.3 197,999 - GFF1599 0.88 +0.8 197,999 - GFF1599 0.88 -0.5 198,073 + -0.9 198,073 + -0.9 198,074 - -1.1 198,074 - +0.6 198,074 - -0.6 198,106 + -0.1 198,106 + +1.7 198,107 - -2.0 198,107 - -0.2 198,107 - -0.8 198,204 + GFF1600 0.10 -1.5 198,715 + GFF1600 0.68 -0.2 198,715 + GFF1600 0.68 -0.7 199,066 + -0.9 199,303 + GFF1601 0.24 +0.8 199,303 + GFF1601 0.24 -0.5 199,318 + GFF1601 0.25 -0.1 199,318 + GFF1601 0.25 +0.5 199,319 - GFF1601 0.25 +0.6 199,319 - GFF1601 0.25 -2.8 199,363 + GFF1601 0.29 -1.0 199,363 + GFF1601 0.29 -0.5 199,364 - GFF1601 0.29 +1.0 199,364 - GFF1601 0.29 +0.2 199,403 - GFF1601 0.32 +2.2 199,487 - GFF1601 0.39 +0.2 199,499 - GFF1601 0.40 -0.6 199,512 + GFF1601 0.41 -0.8 199,534 + GFF1601 0.43 -1.5 199,535 - GFF1601 0.43 -0.4 199,615 + GFF1601 0.50 +0.3 199,675 + GFF1601 0.55 +1.5 199,675 + GFF1601 0.55 +0.4 199,676 - GFF1601 0.55 -0.2 199,795 + GFF1601 0.65 -0.9 199,796 - GFF1601 0.65 +0.0 199,903 + GFF1601 0.74 -2.2 199,904 - GFF1601 0.74 +0.4 199,904 - GFF1601 0.74 -0.2 199,904 - GFF1601 0.74 -1.0
Or see this region's nucleotide sequence