Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1572 and GFF1573 are separated by 101 nucleotides GFF1573 and GFF1574 are separated by 201 nucleotides
GFF1572 - Transcriptional regulator, LysR family, at 172,545 to 173,438
GFF1572
GFF1573 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 173,540 to 174,274
GFF1573
GFF1574 - Alpha-aminoadipate aminotransferase (EC 2.6.1.39) @ Leucine transaminase (EC 2.6.1.6) @ Valine transaminase, at 174,476 to 175,687
GFF1574
Position (kb)
173
174
175 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 172.622 kb on - strand at 172.690 kb on + strand, within GFF1572 at 172.690 kb on + strand, within GFF1572 at 172.691 kb on - strand, within GFF1572 at 172.899 kb on + strand, within GFF1572 at 172.899 kb on + strand, within GFF1572 at 172.900 kb on - strand, within GFF1572 at 172.900 kb on - strand, within GFF1572 at 172.924 kb on + strand, within GFF1572 at 172.925 kb on - strand, within GFF1572 at 173.231 kb on + strand, within GFF1572 at 174.018 kb on - strand, within GFF1573 at 174.018 kb on - strand, within GFF1573 at 174.681 kb on - strand, within GFF1574 at 174.860 kb on + strand, within GFF1574 at 174.861 kb on - strand, within GFF1574 at 174.890 kb on + strand, within GFF1574 at 174.902 kb on + strand, within GFF1574 at 174.978 kb on - strand, within GFF1574 at 174.978 kb on - strand, within GFF1574 at 174.978 kb on - strand, within GFF1574 at 175.208 kb on + strand, within GFF1574 at 175.208 kb on + strand, within GFF1574 at 175.208 kb on + strand, within GFF1574 at 175.208 kb on + strand, within GFF1574 at 175.208 kb on + strand, within GFF1574 at 175.209 kb on - strand, within GFF1574 at 175.209 kb on - strand, within GFF1574 at 175.209 kb on - strand, within GFF1574 at 175.209 kb on - strand, within GFF1574
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 172,622 - -1.0 172,690 + GFF1572 0.16 +0.5 172,690 + GFF1572 0.16 -1.4 172,691 - GFF1572 0.16 -0.8 172,899 + GFF1572 0.40 +0.4 172,899 + GFF1572 0.40 +0.6 172,900 - GFF1572 0.40 -1.0 172,900 - GFF1572 0.40 +0.9 172,924 + GFF1572 0.42 +0.2 172,925 - GFF1572 0.43 +0.2 173,231 + GFF1572 0.77 +0.5 174,018 - GFF1573 0.65 -2.9 174,018 - GFF1573 0.65 -1.1 174,681 - GFF1574 0.17 -0.1 174,860 + GFF1574 0.32 -1.2 174,861 - GFF1574 0.32 +0.2 174,890 + GFF1574 0.34 +0.2 174,902 + GFF1574 0.35 -1.0 174,978 - GFF1574 0.41 -0.6 174,978 - GFF1574 0.41 +0.2 174,978 - GFF1574 0.41 -0.3 175,208 + GFF1574 0.60 -0.9 175,208 + GFF1574 0.60 -0.0 175,208 + GFF1574 0.60 -0.6 175,208 + GFF1574 0.60 -0.3 175,208 + GFF1574 0.60 -0.2 175,209 - GFF1574 0.60 +0.4 175,209 - GFF1574 0.60 -0.1 175,209 - GFF1574 0.60 +0.7 175,209 - GFF1574 0.60 -1.8
Or see this region's nucleotide sequence