Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1489 and GFF1490 are separated by 3 nucleotides GFF1490 and GFF1491 are separated by 35 nucleotides GFF1491 and GFF1492 are separated by 1 nucleotides
GFF1489 - Tetrapartite efflux system, outer membrane factor lipoprotein FusA-like, at 81,910 to 83,373
GFF1489
GFF1490 - Transcriptional regulator, LysR family, at 83,377 to 84,273
GFF1490
GFF1491 - Gluconolactonase, at 84,309 to 85,241
GFF1491
GFF1492 - putative 2-pyrone-4,6-dicarboxylic acid hydrolase, at 85,243 to 86,142
GFF1492
Position (kb)
83
84
85 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 82.471 kb on + strand, within GFF1489 at 82.472 kb on - strand, within GFF1489 at 82.951 kb on + strand, within GFF1489 at 82.952 kb on - strand, within GFF1489 at 83.191 kb on + strand, within GFF1489 at 83.192 kb on - strand, within GFF1489 at 83.192 kb on - strand, within GFF1489 at 83.192 kb on - strand, within GFF1489 at 83.192 kb on - strand, within GFF1489 at 83.192 kb on - strand, within GFF1489 at 83.371 kb on + strand at 83.887 kb on + strand, within GFF1490 at 83.950 kb on + strand, within GFF1490 at 83.951 kb on - strand, within GFF1490 at 83.951 kb on - strand, within GFF1490 at 83.951 kb on - strand, within GFF1490 at 84.007 kb on + strand, within GFF1490 at 84.007 kb on + strand, within GFF1490 at 84.007 kb on + strand, within GFF1490 at 84.008 kb on - strand, within GFF1490 at 84.102 kb on - strand, within GFF1490 at 84.102 kb on - strand, within GFF1490 at 84.187 kb on + strand at 84.187 kb on + strand at 84.188 kb on - strand at 84.188 kb on - strand at 84.310 kb on + strand at 84.310 kb on + strand at 84.311 kb on - strand at 84.437 kb on + strand, within GFF1491 at 84.511 kb on + strand, within GFF1491 at 84.676 kb on + strand, within GFF1491 at 84.676 kb on + strand, within GFF1491 at 84.676 kb on + strand, within GFF1491 at 84.676 kb on + strand, within GFF1491 at 84.820 kb on + strand, within GFF1491 at 84.944 kb on - strand, within GFF1491 at 84.944 kb on - strand, within GFF1491 at 84.944 kb on - strand, within GFF1491 at 85.003 kb on + strand, within GFF1491 at 85.062 kb on + strand, within GFF1491 at 85.062 kb on + strand, within GFF1491 at 85.063 kb on - strand, within GFF1491 at 85.063 kb on - strand, within GFF1491 at 85.063 kb on - strand, within GFF1491 at 85.075 kb on + strand, within GFF1491 at 85.237 kb on + strand at 85.238 kb on - strand at 85.238 kb on - strand at 85.238 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 82,471 + GFF1489 0.38 +0.3 82,472 - GFF1489 0.38 -0.4 82,951 + GFF1489 0.71 -0.0 82,952 - GFF1489 0.71 +0.3 83,191 + GFF1489 0.88 +0.1 83,192 - GFF1489 0.88 -0.9 83,192 - GFF1489 0.88 -0.2 83,192 - GFF1489 0.88 -0.4 83,192 - GFF1489 0.88 -0.3 83,192 - GFF1489 0.88 +1.0 83,371 + +0.1 83,887 + GFF1490 0.57 -0.2 83,950 + GFF1490 0.64 -1.4 83,951 - GFF1490 0.64 +0.5 83,951 - GFF1490 0.64 +0.5 83,951 - GFF1490 0.64 -1.0 84,007 + GFF1490 0.70 -0.1 84,007 + GFF1490 0.70 +4.1 84,007 + GFF1490 0.70 +0.1 84,008 - GFF1490 0.70 -1.1 84,102 - GFF1490 0.81 +2.7 84,102 - GFF1490 0.81 -0.3 84,187 + -0.2 84,187 + -1.1 84,188 - -1.2 84,188 - -3.0 84,310 + +0.2 84,310 + -0.5 84,311 - -0.8 84,437 + GFF1491 0.14 -0.7 84,511 + GFF1491 0.22 -0.3 84,676 + GFF1491 0.39 -0.5 84,676 + GFF1491 0.39 +1.2 84,676 + GFF1491 0.39 +0.8 84,676 + GFF1491 0.39 -1.9 84,820 + GFF1491 0.55 +1.4 84,944 - GFF1491 0.68 -1.4 84,944 - GFF1491 0.68 -0.3 84,944 - GFF1491 0.68 +0.8 85,003 + GFF1491 0.74 -0.8 85,062 + GFF1491 0.81 -0.3 85,062 + GFF1491 0.81 -0.2 85,063 - GFF1491 0.81 -1.1 85,063 - GFF1491 0.81 -0.2 85,063 - GFF1491 0.81 +0.2 85,075 + GFF1491 0.82 +0.4 85,237 + -1.2 85,238 - +0.1 85,238 - +0.4 85,238 - +0.4
Or see this region's nucleotide sequence