Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1374 and GFF1375 are separated by 75 nucleotides GFF1375 and GFF1376 are separated by 57 nucleotides
GFF1374 - hypothetical protein, at 720,322 to 721,572
GFF1374
GFF1375 - Transcriptional regulator, LysR family, at 721,648 to 722,565
GFF1375
GFF1376 - hypothetical protein, at 722,623 to 723,789
GFF1376
Position (kb)
721
722
723 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 720.767 kb on + strand, within GFF1374 at 720.833 kb on + strand, within GFF1374 at 720.833 kb on + strand, within GFF1374 at 720.833 kb on + strand, within GFF1374 at 720.833 kb on + strand, within GFF1374 at 720.833 kb on + strand, within GFF1374 at 720.834 kb on - strand, within GFF1374 at 720.834 kb on - strand, within GFF1374 at 720.834 kb on - strand, within GFF1374 at 720.868 kb on + strand, within GFF1374 at 720.889 kb on - strand, within GFF1374 at 721.701 kb on - strand at 721.701 kb on - strand at 721.701 kb on - strand at 721.736 kb on + strand at 721.736 kb on + strand at 721.736 kb on + strand at 721.736 kb on + strand at 721.736 kb on + strand at 721.736 kb on + strand at 721.737 kb on - strand at 721.737 kb on - strand at 721.737 kb on - strand at 721.739 kb on + strand at 721.739 kb on + strand at 721.739 kb on + strand at 721.739 kb on + strand at 721.740 kb on - strand, within GFF1375 at 721.740 kb on - strand, within GFF1375 at 721.740 kb on - strand, within GFF1375 at 721.742 kb on + strand, within GFF1375 at 721.742 kb on + strand, within GFF1375 at 721.743 kb on - strand, within GFF1375 at 721.743 kb on - strand, within GFF1375 at 721.743 kb on - strand, within GFF1375 at 722.576 kb on + strand at 722.577 kb on - strand at 722.577 kb on - strand at 722.725 kb on + strand at 722.726 kb on - strand at 722.726 kb on - strand at 722.743 kb on + strand, within GFF1376 at 722.743 kb on + strand, within GFF1376 at 722.743 kb on + strand, within GFF1376 at 722.743 kb on + strand, within GFF1376 at 722.743 kb on + strand, within GFF1376 at 722.744 kb on - strand, within GFF1376 at 722.744 kb on - strand, within GFF1376 at 722.744 kb on - strand, within GFF1376 at 722.744 kb on - strand, within GFF1376 at 722.744 kb on - strand, within GFF1376 at 722.842 kb on + strand, within GFF1376 at 722.843 kb on - strand, within GFF1376 at 722.843 kb on - strand, within GFF1376 at 722.911 kb on + strand, within GFF1376 at 722.911 kb on + strand, within GFF1376 at 723.304 kb on + strand, within GFF1376 at 723.304 kb on + strand, within GFF1376 at 723.304 kb on + strand, within GFF1376 at 723.305 kb on - strand, within GFF1376
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 720,767 + GFF1374 0.36 -1.5 720,833 + GFF1374 0.41 +0.4 720,833 + GFF1374 0.41 +0.5 720,833 + GFF1374 0.41 -0.2 720,833 + GFF1374 0.41 +0.9 720,833 + GFF1374 0.41 -0.9 720,834 - GFF1374 0.41 -0.3 720,834 - GFF1374 0.41 +0.2 720,834 - GFF1374 0.41 +0.1 720,868 + GFF1374 0.44 +0.2 720,889 - GFF1374 0.45 -0.7 721,701 - -0.2 721,701 - -2.2 721,701 - -0.1 721,736 + +0.5 721,736 + -1.4 721,736 + +0.2 721,736 + +0.7 721,736 + +0.0 721,736 + -0.1 721,737 - -0.6 721,737 - -0.4 721,737 - -0.1 721,739 + +0.3 721,739 + +0.6 721,739 + -1.9 721,739 + +0.1 721,740 - GFF1375 0.10 -0.6 721,740 - GFF1375 0.10 -0.5 721,740 - GFF1375 0.10 -0.7 721,742 + GFF1375 0.10 -1.1 721,742 + GFF1375 0.10 +0.0 721,743 - GFF1375 0.10 -2.4 721,743 - GFF1375 0.10 -0.1 721,743 - GFF1375 0.10 -0.8 722,576 + +1.0 722,577 - -2.4 722,577 - -0.4 722,725 + -0.3 722,726 - -0.5 722,726 - -2.1 722,743 + GFF1376 0.10 +0.5 722,743 + GFF1376 0.10 +3.8 722,743 + GFF1376 0.10 -0.2 722,743 + GFF1376 0.10 -0.6 722,743 + GFF1376 0.10 -0.3 722,744 - GFF1376 0.10 +0.5 722,744 - GFF1376 0.10 -0.7 722,744 - GFF1376 0.10 -0.5 722,744 - GFF1376 0.10 +0.5 722,744 - GFF1376 0.10 -1.4 722,842 + GFF1376 0.19 +0.1 722,843 - GFF1376 0.19 -0.3 722,843 - GFF1376 0.19 -0.4 722,911 + GFF1376 0.25 -1.0 722,911 + GFF1376 0.25 -0.7 723,304 + GFF1376 0.58 +1.0 723,304 + GFF1376 0.58 +0.1 723,304 + GFF1376 0.58 -0.7 723,305 - GFF1376 0.58 -0.1
Or see this region's nucleotide sequence