Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1164 and GFF1165 are separated by 63 nucleotides GFF1165 and GFF1166 are separated by 117 nucleotides
GFF1164 - 2-isopropylmalate synthase (EC 2.3.3.13), at 478,256 to 479,959
GFF1164
GFF1165 - 2-isopropylmalate synthase (EC 2.3.3.13), at 480,023 to 481,561
GFF1165
GFF1166 - Murein-DD-endopeptidase (EC 3.4.99.-), at 481,679 to 482,884
GFF1166
Position (kb) 480
481
482 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 479.126 kb on + strand, within GFF1164 at 479.126 kb on + strand, within GFF1164 at 479.126 kb on + strand, within GFF1164 at 479.127 kb on - strand, within GFF1164 at 479.127 kb on - strand, within GFF1164 at 479.127 kb on - strand, within GFF1164 at 479.228 kb on + strand, within GFF1164 at 479.228 kb on + strand, within GFF1164 at 479.228 kb on + strand, within GFF1164 at 479.228 kb on + strand, within GFF1164 at 479.228 kb on + strand, within GFF1164 at 479.228 kb on + strand, within GFF1164 at 479.228 kb on + strand, within GFF1164 at 479.228 kb on + strand, within GFF1164 at 479.229 kb on - strand, within GFF1164 at 479.229 kb on - strand, within GFF1164 at 479.229 kb on - strand, within GFF1164 at 479.229 kb on - strand, within GFF1164 at 479.229 kb on - strand, within GFF1164 at 479.246 kb on + strand, within GFF1164 at 479.246 kb on + strand, within GFF1164 at 479.246 kb on + strand, within GFF1164 at 479.246 kb on + strand, within GFF1164 at 479.246 kb on + strand, within GFF1164 at 479.247 kb on - strand, within GFF1164 at 479.247 kb on - strand, within GFF1164 at 479.247 kb on - strand, within GFF1164 at 479.247 kb on - strand, within GFF1164 at 479.247 kb on - strand, within GFF1164 at 479.330 kb on + strand, within GFF1164 at 479.331 kb on - strand, within GFF1164 at 479.331 kb on - strand, within GFF1164 at 479.331 kb on - strand, within GFF1164 at 479.331 kb on - strand, within GFF1164 at 479.331 kb on - strand, within GFF1164 at 479.366 kb on + strand, within GFF1164 at 479.366 kb on + strand, within GFF1164 at 479.366 kb on + strand, within GFF1164 at 479.366 kb on + strand, within GFF1164 at 479.367 kb on - strand, within GFF1164 at 479.367 kb on - strand, within GFF1164 at 479.367 kb on - strand, within GFF1164 at 479.417 kb on + strand, within GFF1164 at 479.417 kb on + strand, within GFF1164 at 479.418 kb on - strand, within GFF1164 at 479.418 kb on - strand, within GFF1164 at 479.418 kb on - strand, within GFF1164 at 479.435 kb on + strand, within GFF1164 at 479.435 kb on + strand, within GFF1164 at 479.436 kb on - strand, within GFF1164 at 479.436 kb on - strand, within GFF1164 at 479.555 kb on + strand, within GFF1164 at 479.555 kb on + strand, within GFF1164 at 479.756 kb on + strand, within GFF1164 at 479.756 kb on + strand, within GFF1164 at 479.756 kb on + strand, within GFF1164 at 479.757 kb on - strand, within GFF1164 at 479.757 kb on - strand, within GFF1164 at 479.757 kb on - strand, within GFF1164 at 479.757 kb on - strand, within GFF1164 at 479.757 kb on - strand, within GFF1164 at 479.757 kb on - strand, within GFF1164 at 479.757 kb on - strand, within GFF1164 at 479.804 kb on + strand at 479.804 kb on + strand at 479.805 kb on - strand at 479.805 kb on - strand at 479.805 kb on - strand at 479.805 kb on - strand at 479.901 kb on + strand at 479.901 kb on + strand at 479.901 kb on + strand at 479.901 kb on + strand at 479.901 kb on + strand at 479.901 kb on + strand at 479.902 kb on - strand at 480.040 kb on - strand at 480.560 kb on + strand, within GFF1165 at 480.560 kb on + strand, within GFF1165 at 480.560 kb on + strand, within GFF1165 at 480.560 kb on + strand, within GFF1165 at 480.560 kb on + strand, within GFF1165 at 480.561 kb on - strand, within GFF1165 at 480.561 kb on - strand, within GFF1165 at 480.785 kb on + strand, within GFF1165 at 480.785 kb on + strand, within GFF1165 at 480.785 kb on + strand, within GFF1165 at 480.785 kb on + strand, within GFF1165 at 480.785 kb on + strand, within GFF1165 at 480.785 kb on + strand, within GFF1165 at 480.786 kb on - strand, within GFF1165 at 480.786 kb on - strand, within GFF1165 at 480.786 kb on - strand, within GFF1165 at 480.786 kb on - strand, within GFF1165 at 480.956 kb on + strand, within GFF1165 at 480.956 kb on + strand, within GFF1165 at 480.956 kb on + strand, within GFF1165 at 480.957 kb on - strand, within GFF1165 at 480.957 kb on - strand, within GFF1165 at 480.957 kb on - strand, within GFF1165 at 480.957 kb on - strand, within GFF1165 at 481.126 kb on + strand, within GFF1165 at 481.126 kb on + strand, within GFF1165 at 481.126 kb on + strand, within GFF1165 at 481.127 kb on - strand, within GFF1165 at 481.127 kb on - strand, within GFF1165 at 481.373 kb on + strand, within GFF1165 at 481.373 kb on + strand, within GFF1165 at 481.373 kb on + strand, within GFF1165 at 481.373 kb on + strand, within GFF1165 at 481.373 kb on + strand, within GFF1165 at 481.373 kb on + strand, within GFF1165 at 481.374 kb on - strand, within GFF1165 at 481.374 kb on - strand, within GFF1165 at 481.374 kb on - strand, within GFF1165 at 481.374 kb on - strand, within GFF1165 at 481.374 kb on - strand, within GFF1165 at 481.380 kb on + strand, within GFF1165 at 481.381 kb on - strand, within GFF1165 at 481.420 kb on + strand at 481.421 kb on - strand at 481.421 kb on - strand at 481.421 kb on - strand at 481.421 kb on - strand at 481.432 kb on - strand at 481.502 kb on + strand at 481.502 kb on + strand at 481.503 kb on - strand at 481.503 kb on - strand at 481.503 kb on - strand at 481.503 kb on - strand at 481.524 kb on + strand at 481.525 kb on - strand at 481.525 kb on - strand at 481.525 kb on - strand at 481.559 kb on + strand at 481.559 kb on + strand at 481.559 kb on + strand at 481.560 kb on - strand at 481.560 kb on - strand at 481.560 kb on - strand at 481.560 kb on - strand at 481.607 kb on + strand at 481.607 kb on + strand at 481.615 kb on - strand at 481.615 kb on - strand at 481.639 kb on + strand at 481.674 kb on + strand at 481.674 kb on + strand at 481.674 kb on + strand at 481.674 kb on + strand at 481.674 kb on + strand at 481.674 kb on + strand at 481.674 kb on + strand at 481.674 kb on + strand at 481.674 kb on + strand at 481.674 kb on + strand at 481.674 kb on + strand at 481.674 kb on + strand at 481.674 kb on + strand at 481.675 kb on - strand at 481.675 kb on - strand at 481.675 kb on - strand at 481.675 kb on - strand at 481.675 kb on - strand at 481.677 kb on + strand at 481.677 kb on + strand at 481.677 kb on + strand at 481.677 kb on + strand at 481.678 kb on - strand at 481.722 kb on + strand at 481.793 kb on - strand at 481.932 kb on + strand, within GFF1166 at 481.932 kb on + strand, within GFF1166 at 482.215 kb on + strand, within GFF1166 at 482.215 kb on + strand, within GFF1166 at 482.215 kb on + strand, within GFF1166 at 482.216 kb on - strand, within GFF1166 at 482.216 kb on - strand, within GFF1166 at 482.216 kb on - strand, within GFF1166 at 482.216 kb on - strand, within GFF1166 at 482.311 kb on + strand, within GFF1166 at 482.311 kb on + strand, within GFF1166 at 482.312 kb on - strand, within GFF1166 at 482.389 kb on + strand, within GFF1166 at 482.389 kb on + strand, within GFF1166 at 482.389 kb on + strand, within GFF1166 at 482.390 kb on - strand, within GFF1166 at 482.390 kb on - strand, within GFF1166 at 482.390 kb on - strand, within GFF1166 at 482.390 kb on - strand, within GFF1166 at 482.390 kb on - strand, within GFF1166 at 482.393 kb on + strand, within GFF1166 at 482.393 kb on + strand, within GFF1166 at 482.393 kb on + strand, within GFF1166 at 482.393 kb on + strand, within GFF1166 at 482.393 kb on + strand, within GFF1166 at 482.393 kb on + strand, within GFF1166 at 482.393 kb on + strand, within GFF1166 at 482.393 kb on + strand, within GFF1166 at 482.393 kb on + strand, within GFF1166 at 482.393 kb on + strand, within GFF1166 at 482.394 kb on - strand, within GFF1166 at 482.394 kb on - strand, within GFF1166 at 482.394 kb on - strand, within GFF1166 at 482.394 kb on - strand, within GFF1166 at 482.394 kb on - strand, within GFF1166 at 482.394 kb on - strand, within GFF1166 at 482.394 kb on - strand, within GFF1166 at 482.394 kb on - strand, within GFF1166 at 482.394 kb on - strand, within GFF1166 at 482.394 kb on - strand, within GFF1166 at 482.394 kb on - strand, within GFF1166 at 482.394 kb on - strand, within GFF1166 at 482.394 kb on - strand, within GFF1166 at 482.394 kb on - strand, within GFF1166 at 482.394 kb on - strand, within GFF1166 at 482.394 kb on - strand, within GFF1166 at 482.394 kb on - strand, within GFF1166 at 482.468 kb on + strand, within GFF1166 at 482.468 kb on + strand, within GFF1166 at 482.468 kb on + strand, within GFF1166 at 482.468 kb on + strand, within GFF1166 at 482.468 kb on + strand, within GFF1166 at 482.469 kb on - strand, within GFF1166 at 482.543 kb on + strand, within GFF1166 at 482.543 kb on + strand, within GFF1166 at 482.543 kb on + strand, within GFF1166 at 482.543 kb on + strand, within GFF1166 at 482.544 kb on - strand, within GFF1166 at 482.544 kb on - strand, within GFF1166 at 482.544 kb on - strand, within GFF1166 at 482.544 kb on - strand, within GFF1166 at 482.544 kb on - strand, within GFF1166
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 479,126 + GFF1164 0.51 -1.3 479,126 + GFF1164 0.51 -0.5 479,126 + GFF1164 0.51 +0.0 479,127 - GFF1164 0.51 +0.4 479,127 - GFF1164 0.51 -1.7 479,127 - GFF1164 0.51 -0.8 479,228 + GFF1164 0.57 -2.1 479,228 + GFF1164 0.57 +0.0 479,228 + GFF1164 0.57 +0.2 479,228 + GFF1164 0.57 -0.1 479,228 + GFF1164 0.57 +0.6 479,228 + GFF1164 0.57 +0.6 479,228 + GFF1164 0.57 +0.5 479,228 + GFF1164 0.57 -0.1 479,229 - GFF1164 0.57 -1.3 479,229 - GFF1164 0.57 -0.0 479,229 - GFF1164 0.57 -1.3 479,229 - GFF1164 0.57 -0.5 479,229 - GFF1164 0.57 +0.1 479,246 + GFF1164 0.58 -0.7 479,246 + GFF1164 0.58 +0.8 479,246 + GFF1164 0.58 -0.0 479,246 + GFF1164 0.58 -1.0 479,246 + GFF1164 0.58 -0.1 479,247 - GFF1164 0.58 +0.9 479,247 - GFF1164 0.58 -0.4 479,247 - GFF1164 0.58 -0.1 479,247 - GFF1164 0.58 +0.1 479,247 - GFF1164 0.58 +0.8 479,330 + GFF1164 0.63 -0.1 479,331 - GFF1164 0.63 +0.2 479,331 - GFF1164 0.63 -1.5 479,331 - GFF1164 0.63 +0.5 479,331 - GFF1164 0.63 -0.1 479,331 - GFF1164 0.63 +0.9 479,366 + GFF1164 0.65 +0.1 479,366 + GFF1164 0.65 +0.1 479,366 + GFF1164 0.65 +0.4 479,366 + GFF1164 0.65 -2.0 479,367 - GFF1164 0.65 +0.2 479,367 - GFF1164 0.65 -2.2 479,367 - GFF1164 0.65 -0.3 479,417 + GFF1164 0.68 -1.0 479,417 + GFF1164 0.68 -0.5 479,418 - GFF1164 0.68 -1.3 479,418 - GFF1164 0.68 +0.7 479,418 - GFF1164 0.68 +0.1 479,435 + GFF1164 0.69 +0.1 479,435 + GFF1164 0.69 -0.8 479,436 - GFF1164 0.69 +0.6 479,436 - GFF1164 0.69 -0.8 479,555 + GFF1164 0.76 +0.4 479,555 + GFF1164 0.76 -0.5 479,756 + GFF1164 0.88 +0.8 479,756 + GFF1164 0.88 +0.7 479,756 + GFF1164 0.88 -0.9 479,757 - GFF1164 0.88 -0.1 479,757 - GFF1164 0.88 -0.1 479,757 - GFF1164 0.88 +0.8 479,757 - GFF1164 0.88 -0.1 479,757 - GFF1164 0.88 +1.5 479,757 - GFF1164 0.88 -0.8 479,757 - GFF1164 0.88 +0.1 479,804 + -2.2 479,804 + +0.7 479,805 - +0.5 479,805 - -0.5 479,805 - -1.5 479,805 - +0.7 479,901 + +1.1 479,901 + +0.1 479,901 + -0.2 479,901 + -0.5 479,901 + -0.1 479,901 + -0.2 479,902 - -0.3 480,040 - -0.6 480,560 + GFF1165 0.35 -0.5 480,560 + GFF1165 0.35 +0.0 480,560 + GFF1165 0.35 -0.2 480,560 + GFF1165 0.35 -0.8 480,560 + GFF1165 0.35 +0.8 480,561 - GFF1165 0.35 +1.4 480,561 - GFF1165 0.35 -0.2 480,785 + GFF1165 0.50 -0.4 480,785 + GFF1165 0.50 -0.2 480,785 + GFF1165 0.50 -2.1 480,785 + GFF1165 0.50 -0.1 480,785 + GFF1165 0.50 -1.1 480,785 + GFF1165 0.50 -0.7 480,786 - GFF1165 0.50 +0.7 480,786 - GFF1165 0.50 +0.0 480,786 - GFF1165 0.50 +2.5 480,786 - GFF1165 0.50 +0.2 480,956 + GFF1165 0.61 -0.2 480,956 + GFF1165 0.61 +0.3 480,956 + GFF1165 0.61 -0.5 480,957 - GFF1165 0.61 -0.4 480,957 - GFF1165 0.61 -0.5 480,957 - GFF1165 0.61 -0.3 480,957 - GFF1165 0.61 +0.5 481,126 + GFF1165 0.72 -0.6 481,126 + GFF1165 0.72 -0.1 481,126 + GFF1165 0.72 -0.6 481,127 - GFF1165 0.72 -0.4 481,127 - GFF1165 0.72 -0.4 481,373 + GFF1165 0.88 -0.6 481,373 + GFF1165 0.88 +0.4 481,373 + GFF1165 0.88 -0.2 481,373 + GFF1165 0.88 +0.8 481,373 + GFF1165 0.88 +0.2 481,373 + GFF1165 0.88 +0.3 481,374 - GFF1165 0.88 -0.4 481,374 - GFF1165 0.88 +0.1 481,374 - GFF1165 0.88 +0.1 481,374 - GFF1165 0.88 +0.7 481,374 - GFF1165 0.88 -0.1 481,380 + GFF1165 0.88 -0.7 481,381 - GFF1165 0.88 -0.1 481,420 + -3.4 481,421 - -2.1 481,421 - -0.3 481,421 - -0.1 481,421 - -0.5 481,432 - +0.5 481,502 + -0.6 481,502 + +0.1 481,503 - -1.1 481,503 - -0.2 481,503 - +0.2 481,503 - -1.2 481,524 + +0.1 481,525 - +1.0 481,525 - -0.1 481,525 - -1.3 481,559 + -2.6 481,559 + -2.0 481,559 + -0.0 481,560 - -0.1 481,560 - +0.5 481,560 - -0.5 481,560 - -1.6 481,607 + +0.1 481,607 + +0.2 481,615 - +0.9 481,615 - -1.8 481,639 + +0.1 481,674 + -0.4 481,674 + +0.3 481,674 + +0.2 481,674 + -0.0 481,674 + -0.8 481,674 + -0.4 481,674 + -1.8 481,674 + +0.2 481,674 + +0.0 481,674 + -1.0 481,674 + +0.6 481,674 + +0.5 481,674 + +0.0 481,675 - -0.6 481,675 - +0.4 481,675 - +0.0 481,675 - +0.1 481,675 - -0.2 481,677 + +0.2 481,677 + +0.8 481,677 + -0.2 481,677 + -0.3 481,678 - -0.5 481,722 + +0.3 481,793 - -1.1 481,932 + GFF1166 0.21 +0.5 481,932 + GFF1166 0.21 +0.6 482,215 + GFF1166 0.44 -0.7 482,215 + GFF1166 0.44 +1.2 482,215 + GFF1166 0.44 -0.3 482,216 - GFF1166 0.45 -0.0 482,216 - GFF1166 0.45 -2.9 482,216 - GFF1166 0.45 -0.4 482,216 - GFF1166 0.45 -0.1 482,311 + GFF1166 0.52 +0.9 482,311 + GFF1166 0.52 +1.6 482,312 - GFF1166 0.52 -0.4 482,389 + GFF1166 0.59 +0.3 482,389 + GFF1166 0.59 -0.3 482,389 + GFF1166 0.59 +0.6 482,390 - GFF1166 0.59 -0.5 482,390 - GFF1166 0.59 -0.1 482,390 - GFF1166 0.59 +0.6 482,390 - GFF1166 0.59 -0.1 482,390 - GFF1166 0.59 +0.3 482,393 + GFF1166 0.59 +0.3 482,393 + GFF1166 0.59 -0.7 482,393 + GFF1166 0.59 -0.2 482,393 + GFF1166 0.59 +0.5 482,393 + GFF1166 0.59 -0.4 482,393 + GFF1166 0.59 -0.3 482,393 + GFF1166 0.59 -2.2 482,393 + GFF1166 0.59 -0.2 482,393 + GFF1166 0.59 -0.8 482,393 + GFF1166 0.59 -1.1 482,394 - GFF1166 0.59 -0.1 482,394 - GFF1166 0.59 -0.9 482,394 - GFF1166 0.59 -0.3 482,394 - GFF1166 0.59 -1.0 482,394 - GFF1166 0.59 -0.5 482,394 - GFF1166 0.59 -0.3 482,394 - GFF1166 0.59 +0.7 482,394 - GFF1166 0.59 -0.2 482,394 - GFF1166 0.59 -0.8 482,394 - GFF1166 0.59 -0.0 482,394 - GFF1166 0.59 -0.1 482,394 - GFF1166 0.59 -0.4 482,394 - GFF1166 0.59 -0.3 482,394 - GFF1166 0.59 -1.0 482,394 - GFF1166 0.59 -0.2 482,394 - GFF1166 0.59 -0.1 482,394 - GFF1166 0.59 -0.5 482,468 + GFF1166 0.65 -0.8 482,468 + GFF1166 0.65 -0.9 482,468 + GFF1166 0.65 +0.2 482,468 + GFF1166 0.65 +0.2 482,468 + GFF1166 0.65 -1.1 482,469 - GFF1166 0.66 -0.9 482,543 + GFF1166 0.72 -1.7 482,543 + GFF1166 0.72 -1.3 482,543 + GFF1166 0.72 -0.5 482,543 + GFF1166 0.72 -1.2 482,544 - GFF1166 0.72 -0.8 482,544 - GFF1166 0.72 -1.8 482,544 - GFF1166 0.72 +1.1 482,544 - GFF1166 0.72 -0.3 482,544 - GFF1166 0.72 +0.3
Or see this region's nucleotide sequence