Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1152 and GFF1153 are separated by 31 nucleotides GFF1153 and GFF1154 overlap by 4 nucleotides GFF1154 and GFF1155 are separated by 14 nucleotides GFF1155 and GFF1156 are separated by 16 nucleotides
GFF1152 - Lysine decarboxylase family, at 469,555 to 470,145
GFF1152
GFF1153 - Diacylglycerol kinase (EC 2.7.1.107), at 470,177 to 470,584
GFF1153
GFF1154 - Putative transmembrane protein, at 470,581 to 471,147
GFF1154
GFF1155 - Putative transmembrane protein, at 471,162 to 471,986
GFF1155
GFF1156 - Putative transmembrane protein, at 472,003 to 472,425
GFF1156
Position (kb)
470
471
472 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 469.721 kb on + strand, within GFF1152 at 469.721 kb on + strand, within GFF1152 at 469.721 kb on + strand, within GFF1152 at 469.721 kb on + strand, within GFF1152 at 469.721 kb on + strand, within GFF1152 at 469.721 kb on + strand, within GFF1152 at 469.721 kb on + strand, within GFF1152 at 469.721 kb on + strand, within GFF1152 at 469.722 kb on - strand, within GFF1152 at 469.722 kb on - strand, within GFF1152 at 469.724 kb on + strand, within GFF1152 at 469.725 kb on - strand, within GFF1152 at 469.725 kb on - strand, within GFF1152 at 470.025 kb on - strand, within GFF1152 at 470.025 kb on - strand, within GFF1152 at 470.025 kb on - strand, within GFF1152 at 470.025 kb on - strand, within GFF1152 at 470.025 kb on - strand, within GFF1152 at 470.025 kb on - strand, within GFF1152 at 470.025 kb on - strand, within GFF1152 at 470.117 kb on + strand at 470.117 kb on + strand at 470.117 kb on + strand at 470.117 kb on + strand at 470.117 kb on + strand at 470.117 kb on + strand at 470.117 kb on + strand at 470.117 kb on + strand at 470.118 kb on - strand at 470.118 kb on - strand at 470.118 kb on - strand at 470.118 kb on - strand at 470.118 kb on - strand at 470.118 kb on - strand at 470.118 kb on - strand at 470.783 kb on + strand, within GFF1154 at 470.840 kb on - strand, within GFF1154 at 470.841 kb on + strand, within GFF1154 at 470.841 kb on + strand, within GFF1154 at 470.841 kb on + strand, within GFF1154 at 470.842 kb on - strand, within GFF1154 at 470.842 kb on - strand, within GFF1154 at 470.872 kb on + strand, within GFF1154 at 470.894 kb on + strand, within GFF1154 at 470.894 kb on + strand, within GFF1154 at 470.894 kb on + strand, within GFF1154 at 470.894 kb on + strand, within GFF1154 at 470.894 kb on + strand, within GFF1154 at 470.894 kb on + strand, within GFF1154 at 470.894 kb on + strand, within GFF1154 at 470.895 kb on - strand, within GFF1154 at 470.895 kb on - strand, within GFF1154 at 470.895 kb on - strand, within GFF1154 at 470.895 kb on - strand, within GFF1154 at 470.909 kb on - strand, within GFF1154 at 471.026 kb on + strand, within GFF1154 at 471.026 kb on + strand, within GFF1154 at 471.026 kb on + strand, within GFF1154 at 471.026 kb on + strand, within GFF1154 at 471.026 kb on + strand, within GFF1154 at 471.026 kb on + strand, within GFF1154 at 471.026 kb on + strand, within GFF1154 at 471.026 kb on + strand, within GFF1154 at 471.027 kb on - strand, within GFF1154 at 471.027 kb on - strand, within GFF1154 at 471.027 kb on - strand, within GFF1154 at 471.027 kb on - strand, within GFF1154 at 471.027 kb on - strand, within GFF1154 at 471.027 kb on - strand, within GFF1154 at 471.210 kb on + strand at 471.210 kb on + strand at 471.211 kb on - strand at 471.211 kb on - strand at 471.211 kb on - strand at 471.211 kb on - strand at 471.335 kb on + strand, within GFF1155 at 471.526 kb on + strand, within GFF1155 at 471.526 kb on + strand, within GFF1155 at 471.526 kb on + strand, within GFF1155 at 471.527 kb on - strand, within GFF1155 at 471.527 kb on - strand, within GFF1155 at 471.527 kb on - strand, within GFF1155 at 471.527 kb on - strand, within GFF1155 at 471.527 kb on - strand, within GFF1155 at 471.679 kb on + strand, within GFF1155 at 471.679 kb on + strand, within GFF1155 at 471.679 kb on + strand, within GFF1155 at 471.680 kb on - strand, within GFF1155 at 471.680 kb on - strand, within GFF1155 at 471.680 kb on - strand, within GFF1155 at 471.680 kb on - strand, within GFF1155 at 471.956 kb on + strand at 471.956 kb on + strand at 471.956 kb on + strand at 471.956 kb on + strand at 471.956 kb on + strand at 471.956 kb on + strand at 471.956 kb on + strand at 471.957 kb on - strand at 471.957 kb on - strand at 471.957 kb on - strand at 471.957 kb on - strand at 472.053 kb on + strand, within GFF1156 at 472.054 kb on - strand, within GFF1156 at 472.054 kb on - strand, within GFF1156 at 472.135 kb on + strand, within GFF1156 at 472.135 kb on + strand, within GFF1156 at 472.135 kb on + strand, within GFF1156 at 472.136 kb on - strand, within GFF1156 at 472.136 kb on - strand, within GFF1156 at 472.136 kb on - strand, within GFF1156
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 469,721 + GFF1152 0.28 +0.9 469,721 + GFF1152 0.28 -1.1 469,721 + GFF1152 0.28 -1.1 469,721 + GFF1152 0.28 +1.2 469,721 + GFF1152 0.28 -0.2 469,721 + GFF1152 0.28 +0.1 469,721 + GFF1152 0.28 +0.3 469,721 + GFF1152 0.28 -0.0 469,722 - GFF1152 0.28 +0.3 469,722 - GFF1152 0.28 +0.6 469,724 + GFF1152 0.29 +0.1 469,725 - GFF1152 0.29 -1.0 469,725 - GFF1152 0.29 -0.2 470,025 - GFF1152 0.80 -0.1 470,025 - GFF1152 0.80 -1.6 470,025 - GFF1152 0.80 -0.4 470,025 - GFF1152 0.80 -0.3 470,025 - GFF1152 0.80 -0.4 470,025 - GFF1152 0.80 +0.9 470,025 - GFF1152 0.80 +0.5 470,117 + -0.6 470,117 + +1.5 470,117 + -1.7 470,117 + +0.1 470,117 + -0.0 470,117 + -1.3 470,117 + -0.9 470,117 + +0.1 470,118 - +0.4 470,118 - -2.2 470,118 - -0.1 470,118 - -0.6 470,118 - -0.7 470,118 - -0.3 470,118 - +1.0 470,783 + GFF1154 0.36 -4.0 470,840 - GFF1154 0.46 -0.0 470,841 + GFF1154 0.46 -1.1 470,841 + GFF1154 0.46 -1.6 470,841 + GFF1154 0.46 -0.7 470,842 - GFF1154 0.46 -0.5 470,842 - GFF1154 0.46 -0.6 470,872 + GFF1154 0.51 -0.8 470,894 + GFF1154 0.55 -0.8 470,894 + GFF1154 0.55 -0.9 470,894 + GFF1154 0.55 -1.3 470,894 + GFF1154 0.55 -1.0 470,894 + GFF1154 0.55 -1.0 470,894 + GFF1154 0.55 -0.8 470,894 + GFF1154 0.55 -0.1 470,895 - GFF1154 0.55 +0.8 470,895 - GFF1154 0.55 +0.1 470,895 - GFF1154 0.55 -1.6 470,895 - GFF1154 0.55 -1.0 470,909 - GFF1154 0.58 -1.0 471,026 + GFF1154 0.78 -0.5 471,026 + GFF1154 0.78 -1.1 471,026 + GFF1154 0.78 -0.6 471,026 + GFF1154 0.78 +0.2 471,026 + GFF1154 0.78 -1.0 471,026 + GFF1154 0.78 -0.6 471,026 + GFF1154 0.78 -0.3 471,026 + GFF1154 0.78 -1.7 471,027 - GFF1154 0.79 -2.7 471,027 - GFF1154 0.79 -2.1 471,027 - GFF1154 0.79 -0.8 471,027 - GFF1154 0.79 -1.0 471,027 - GFF1154 0.79 -0.3 471,027 - GFF1154 0.79 -1.5 471,210 + -0.7 471,210 + -0.3 471,211 - +0.1 471,211 - +0.5 471,211 - -0.3 471,211 - +0.1 471,335 + GFF1155 0.21 -1.0 471,526 + GFF1155 0.44 -1.0 471,526 + GFF1155 0.44 -0.2 471,526 + GFF1155 0.44 -0.2 471,527 - GFF1155 0.44 -0.1 471,527 - GFF1155 0.44 -0.5 471,527 - GFF1155 0.44 -1.7 471,527 - GFF1155 0.44 -1.5 471,527 - GFF1155 0.44 +0.3 471,679 + GFF1155 0.63 -0.1 471,679 + GFF1155 0.63 +1.5 471,679 + GFF1155 0.63 -1.2 471,680 - GFF1155 0.63 -0.8 471,680 - GFF1155 0.63 -1.1 471,680 - GFF1155 0.63 -1.1 471,680 - GFF1155 0.63 -0.1 471,956 + -1.0 471,956 + +0.9 471,956 + -0.2 471,956 + +0.3 471,956 + +0.6 471,956 + -0.4 471,956 + +0.6 471,957 - -1.6 471,957 - +0.6 471,957 - +0.1 471,957 - +0.2 472,053 + GFF1156 0.12 +1.1 472,054 - GFF1156 0.12 -0.8 472,054 - GFF1156 0.12 +1.9 472,135 + GFF1156 0.31 -0.3 472,135 + GFF1156 0.31 -0.5 472,135 + GFF1156 0.31 +1.2 472,136 - GFF1156 0.31 +0.5 472,136 - GFF1156 0.31 +0.5 472,136 - GFF1156 0.31 -0.8
Or see this region's nucleotide sequence