Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF900 and GFF901 are separated by 13 nucleotides GFF901 and GFF902 are separated by 49 nucleotides GFF902 and GFF903 are separated by 65 nucleotides
GFF900 - hypothetical protein, at 211,660 to 212,676
GFF900
GFF901 - no description, at 212,690 to 213,325
GFF901
GFF902 - Transcriptional regulator, AsnC family, at 213,375 to 213,869
GFF902
GFF903 - Acetolactate synthase large subunit (EC 2.2.1.6), at 213,935 to 215,713
GFF903
Position (kb) 213
214 Strain fitness (log2 ratio) -2
-1
0
1
2 at 212.384 kb on - strand, within GFF900 at 213.069 kb on + strand, within GFF901 at 213.070 kb on - strand, within GFF901 at 213.070 kb on - strand, within GFF901 at 213.454 kb on + strand, within GFF902 at 213.455 kb on - strand, within GFF902 at 213.455 kb on - strand, within GFF902 at 213.481 kb on + strand, within GFF902 at 213.514 kb on + strand, within GFF902 at 213.514 kb on + strand, within GFF902 at 213.514 kb on + strand, within GFF902 at 213.514 kb on + strand, within GFF902 at 213.514 kb on + strand, within GFF902 at 213.514 kb on + strand, within GFF902 at 213.514 kb on + strand, within GFF902 at 213.515 kb on - strand, within GFF902 at 213.515 kb on - strand, within GFF902 at 213.515 kb on - strand, within GFF902 at 213.515 kb on - strand, within GFF902 at 213.515 kb on - strand, within GFF902 at 213.515 kb on - strand, within GFF902 at 213.515 kb on - strand, within GFF902 at 213.515 kb on - strand, within GFF902 at 213.515 kb on - strand, within GFF902 at 213.515 kb on - strand, within GFF902 at 213.517 kb on + strand, within GFF902 at 213.517 kb on + strand, within GFF902 at 213.517 kb on + strand, within GFF902 at 213.518 kb on - strand, within GFF902 at 213.518 kb on - strand, within GFF902 at 213.518 kb on - strand, within GFF902 at 213.523 kb on + strand, within GFF902 at 213.541 kb on + strand, within GFF902 at 213.541 kb on + strand, within GFF902 at 213.541 kb on + strand, within GFF902 at 213.541 kb on + strand, within GFF902 at 213.541 kb on + strand, within GFF902 at 213.542 kb on - strand, within GFF902 at 213.542 kb on - strand, within GFF902 at 213.542 kb on - strand, within GFF902 at 213.571 kb on - strand, within GFF902 at 213.571 kb on - strand, within GFF902 at 213.589 kb on + strand, within GFF902 at 213.590 kb on - strand, within GFF902 at 213.590 kb on - strand, within GFF902 at 213.590 kb on - strand, within GFF902 at 213.679 kb on + strand, within GFF902 at 213.680 kb on - strand, within GFF902 at 213.680 kb on - strand, within GFF902 at 213.903 kb on + strand at 213.903 kb on + strand at 213.904 kb on - strand at 213.951 kb on + strand at 213.951 kb on + strand at 213.951 kb on + strand at 213.952 kb on - strand at 213.952 kb on - strand at 213.952 kb on - strand at 213.952 kb on - strand at 214.065 kb on - strand at 214.092 kb on - strand at 214.092 kb on - strand at 214.172 kb on + strand, within GFF903 at 214.173 kb on - strand, within GFF903 at 214.173 kb on - strand, within GFF903 at 214.173 kb on - strand, within GFF903 at 214.173 kb on - strand, within GFF903 at 214.622 kb on + strand, within GFF903 at 214.622 kb on + strand, within GFF903 at 214.623 kb on - strand, within GFF903
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 212,384 - GFF900 0.71 +0.3 213,069 + GFF901 0.60 +0.6 213,070 - GFF901 0.60 -1.5 213,070 - GFF901 0.60 +1.5 213,454 + GFF902 0.16 -0.5 213,455 - GFF902 0.16 -0.7 213,455 - GFF902 0.16 -0.2 213,481 + GFF902 0.21 -0.8 213,514 + GFF902 0.28 +0.5 213,514 + GFF902 0.28 +0.9 213,514 + GFF902 0.28 +0.1 213,514 + GFF902 0.28 -1.2 213,514 + GFF902 0.28 +0.1 213,514 + GFF902 0.28 +0.7 213,514 + GFF902 0.28 +0.3 213,515 - GFF902 0.28 +0.5 213,515 - GFF902 0.28 +0.7 213,515 - GFF902 0.28 -0.8 213,515 - GFF902 0.28 -1.2 213,515 - GFF902 0.28 -0.4 213,515 - GFF902 0.28 -0.2 213,515 - GFF902 0.28 -0.3 213,515 - GFF902 0.28 -0.4 213,515 - GFF902 0.28 -0.5 213,515 - GFF902 0.28 +0.4 213,517 + GFF902 0.29 -0.5 213,517 + GFF902 0.29 +0.1 213,517 + GFF902 0.29 -0.5 213,518 - GFF902 0.29 -0.6 213,518 - GFF902 0.29 +0.9 213,518 - GFF902 0.29 +0.3 213,523 + GFF902 0.30 +0.6 213,541 + GFF902 0.34 +0.3 213,541 + GFF902 0.34 -0.5 213,541 + GFF902 0.34 -1.1 213,541 + GFF902 0.34 -0.7 213,541 + GFF902 0.34 -0.8 213,542 - GFF902 0.34 -0.9 213,542 - GFF902 0.34 -0.4 213,542 - GFF902 0.34 +0.6 213,571 - GFF902 0.40 +0.9 213,571 - GFF902 0.40 +0.8 213,589 + GFF902 0.43 -1.4 213,590 - GFF902 0.43 -1.1 213,590 - GFF902 0.43 -0.2 213,590 - GFF902 0.43 -0.7 213,679 + GFF902 0.61 +1.3 213,680 - GFF902 0.62 +0.4 213,680 - GFF902 0.62 -1.3 213,903 + +2.5 213,903 + -0.6 213,904 - -1.0 213,951 + +1.5 213,951 + -1.2 213,951 + -1.3 213,952 - -0.7 213,952 - +0.3 213,952 - -0.4 213,952 - -0.4 214,065 - -0.3 214,092 - -1.8 214,092 - +0.4 214,172 + GFF903 0.13 +0.0 214,173 - GFF903 0.13 -0.7 214,173 - GFF903 0.13 +0.1 214,173 - GFF903 0.13 +0.3 214,173 - GFF903 0.13 -0.3 214,622 + GFF903 0.39 -1.1 214,622 + GFF903 0.39 -0.4 214,623 - GFF903 0.39 +0.3
Or see this region's nucleotide sequence