Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF836 and GFF837 are separated by 34 nucleotides GFF837 and GFF838 overlap by 4 nucleotides GFF838 and GFF839 are separated by 317 nucleotides
GFF836 - Vitamin B12 ABC transporter, substrate-binding protein BtuF, at 141,628 to 142,569
GFF836
GFF837 - FIG01002808: hypothetical protein, at 142,604 to 143,455
GFF837
GFF838 - hypothetical protein, at 143,452 to 144,552
GFF838
GFF839 - Retron-type RNA-directed DNA polymerase (EC 2.7.7.49), at 144,870 to 146,816
GFF839
Position (kb)
143
144
145 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 142.605 kb on + strand at 142.605 kb on + strand at 142.605 kb on + strand at 142.606 kb on - strand at 142.758 kb on + strand, within GFF837 at 142.758 kb on + strand, within GFF837 at 142.759 kb on - strand, within GFF837 at 142.759 kb on - strand, within GFF837 at 142.782 kb on + strand, within GFF837 at 142.782 kb on + strand, within GFF837 at 143.278 kb on - strand, within GFF837 at 143.319 kb on + strand, within GFF837 at 143.319 kb on + strand, within GFF837 at 143.319 kb on + strand, within GFF837 at 143.319 kb on + strand, within GFF837 at 143.320 kb on - strand, within GFF837 at 143.361 kb on + strand, within GFF837 at 143.361 kb on + strand, within GFF837 at 143.361 kb on + strand, within GFF837 at 143.361 kb on + strand, within GFF837 at 143.362 kb on - strand, within GFF837 at 143.362 kb on - strand, within GFF837 at 143.362 kb on - strand, within GFF837 at 143.495 kb on + strand at 143.496 kb on - strand at 143.525 kb on + strand at 143.804 kb on + strand, within GFF838 at 143.849 kb on + strand, within GFF838 at 143.849 kb on + strand, within GFF838 at 143.849 kb on + strand, within GFF838 at 143.850 kb on - strand, within GFF838 at 143.864 kb on + strand, within GFF838 at 143.865 kb on - strand, within GFF838 at 144.269 kb on + strand, within GFF838 at 144.531 kb on - strand at 144.599 kb on + strand at 144.763 kb on + strand at 144.763 kb on + strand at 144.763 kb on + strand at 144.764 kb on - strand at 144.783 kb on + strand at 144.784 kb on - strand at 145.036 kb on + strand at 145.036 kb on + strand at 145.036 kb on + strand at 145.036 kb on + strand at 145.036 kb on + strand at 145.037 kb on - strand at 145.037 kb on - strand at 145.037 kb on - strand at 145.037 kb on - strand at 145.427 kb on - strand, within GFF839 at 145.427 kb on - strand, within GFF839
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 142,605 + +1.4 142,605 + -1.2 142,605 + -1.8 142,606 - -0.9 142,758 + GFF837 0.18 -0.4 142,758 + GFF837 0.18 +0.4 142,759 - GFF837 0.18 -0.9 142,759 - GFF837 0.18 +1.3 142,782 + GFF837 0.21 +0.5 142,782 + GFF837 0.21 -0.6 143,278 - GFF837 0.79 -1.2 143,319 + GFF837 0.84 -0.7 143,319 + GFF837 0.84 -1.1 143,319 + GFF837 0.84 +0.7 143,319 + GFF837 0.84 -2.2 143,320 - GFF837 0.84 -1.1 143,361 + GFF837 0.89 +1.0 143,361 + GFF837 0.89 -0.1 143,361 + GFF837 0.89 -0.7 143,361 + GFF837 0.89 +0.3 143,362 - GFF837 0.89 -0.1 143,362 - GFF837 0.89 -0.8 143,362 - GFF837 0.89 -1.5 143,495 + +0.1 143,496 - -0.4 143,525 + +1.9 143,804 + GFF838 0.32 +0.7 143,849 + GFF838 0.36 +0.2 143,849 + GFF838 0.36 +2.8 143,849 + GFF838 0.36 -0.1 143,850 - GFF838 0.36 +0.3 143,864 + GFF838 0.37 +0.3 143,865 - GFF838 0.38 -0.1 144,269 + GFF838 0.74 +0.0 144,531 - -0.2 144,599 + -0.6 144,763 + -0.6 144,763 + -0.9 144,763 + +0.7 144,764 - +1.9 144,783 + -0.7 144,784 - -1.0 145,036 + +0.3 145,036 + -0.7 145,036 + -1.1 145,036 + -0.7 145,036 + -0.1 145,037 - +1.9 145,037 - -0.2 145,037 - -0.2 145,037 - -0.5 145,427 - GFF839 0.29 -0.8 145,427 - GFF839 0.29 +1.3
Or see this region's nucleotide sequence