Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7389 and GFF7390 are separated by 9 nucleotides GFF7390 and GFF7391 are separated by 27 nucleotides
GFF7389 - Cyclohexanone monooxygenase (EC 1.14.13.22), at 6,367 to 7,797
GFF7389
GFF7390 - Aldehyde dehydrogenase (EC 1.2.1.3), at 7,807 to 9,300
GFF7390
GFF7391 - hypothetical protein, at 9,328 to 10,266
GFF7391
Position (kb)
7
8
9
10 Strain fitness (log2 ratio)
-1
0
1
2 at 6.994 kb on + strand, within GFF7389 at 6.995 kb on - strand, within GFF7389 at 7.133 kb on - strand, within GFF7389 at 7.489 kb on + strand, within GFF7389 at 7.489 kb on + strand, within GFF7389 at 7.525 kb on + strand, within GFF7389 at 7.525 kb on + strand, within GFF7389 at 7.525 kb on + strand, within GFF7389 at 7.550 kb on - strand, within GFF7389 at 7.550 kb on - strand, within GFF7389 at 7.628 kb on - strand, within GFF7389 at 7.690 kb on + strand at 7.690 kb on + strand at 7.726 kb on + strand at 7.898 kb on - strand at 7.898 kb on - strand at 8.150 kb on + strand, within GFF7390 at 8.150 kb on + strand, within GFF7390 at 8.151 kb on - strand, within GFF7390 at 8.151 kb on - strand, within GFF7390 at 8.241 kb on + strand, within GFF7390 at 8.242 kb on - strand, within GFF7390 at 8.242 kb on - strand, within GFF7390 at 8.503 kb on + strand, within GFF7390 at 8.503 kb on + strand, within GFF7390 at 8.504 kb on - strand, within GFF7390 at 8.657 kb on + strand, within GFF7390 at 8.763 kb on + strand, within GFF7390 at 8.932 kb on + strand, within GFF7390 at 9.014 kb on + strand, within GFF7390 at 9.160 kb on + strand at 9.179 kb on - strand at 9.254 kb on - strand at 9.551 kb on - strand, within GFF7391 at 9.551 kb on - strand, within GFF7391 at 9.580 kb on + strand, within GFF7391 at 9.734 kb on + strand, within GFF7391 at 9.735 kb on + strand, within GFF7391 at 9.824 kb on + strand, within GFF7391 at 9.825 kb on - strand, within GFF7391 at 10.047 kb on + strand, within GFF7391 at 10.048 kb on - strand, within GFF7391 at 10.210 kb on - strand at 10.236 kb on + strand at 10.240 kb on - strand at 10.248 kb on + strand at 10.248 kb on + strand at 10.249 kb on - strand at 10.250 kb on + strand at 10.253 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 6,994 + GFF7389 0.44 -0.1 6,995 - GFF7389 0.44 -0.3 7,133 - GFF7389 0.54 -0.6 7,489 + GFF7389 0.78 -0.4 7,489 + GFF7389 0.78 +1.2 7,525 + GFF7389 0.81 -0.8 7,525 + GFF7389 0.81 +1.3 7,525 + GFF7389 0.81 +2.2 7,550 - GFF7389 0.83 -0.6 7,550 - GFF7389 0.83 -0.9 7,628 - GFF7389 0.88 -0.1 7,690 + -1.5 7,690 + +0.5 7,726 + -1.0 7,898 - +0.6 7,898 - +0.9 8,150 + GFF7390 0.23 +0.6 8,150 + GFF7390 0.23 -1.0 8,151 - GFF7390 0.23 +0.8 8,151 - GFF7390 0.23 -0.3 8,241 + GFF7390 0.29 +0.9 8,242 - GFF7390 0.29 +2.2 8,242 - GFF7390 0.29 +0.3 8,503 + GFF7390 0.47 +0.7 8,503 + GFF7390 0.47 -1.4 8,504 - GFF7390 0.47 -0.2 8,657 + GFF7390 0.57 -0.4 8,763 + GFF7390 0.64 -1.3 8,932 + GFF7390 0.75 -0.2 9,014 + GFF7390 0.81 +1.6 9,160 + +0.4 9,179 - +0.1 9,254 - -0.8 9,551 - GFF7391 0.24 -0.3 9,551 - GFF7391 0.24 +0.2 9,580 + GFF7391 0.27 -0.4 9,734 + GFF7391 0.43 -0.4 9,735 + GFF7391 0.43 -0.4 9,824 + GFF7391 0.53 +0.2 9,825 - GFF7391 0.53 -1.3 10,047 + GFF7391 0.77 +1.2 10,048 - GFF7391 0.77 +0.4 10,210 - -1.3 10,236 + -0.4 10,240 - -0.1 10,248 + -0.4 10,248 + -0.5 10,249 - -0.5 10,250 + +0.0 10,253 - -0.6
Or see this region's nucleotide sequence