Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7238 and GFF7239 are separated by 126 nucleotides GFF7239 and GFF7240 are separated by 21 nucleotides GFF7240 and GFF7241 are separated by 156 nucleotides GFF7241 and GFF7242 are separated by 16 nucleotides
GFF7238 - no description, at 19,736 to 20,242
GFF7238
GFF7239 - Repair of Iron Centers di-iron protein, at 20,369 to 20,953
GFF7239
GFF7240 - Polyhydroxyalkanoic acid synthase, at 20,975 to 22,636
GFF7240
GFF7241 - no description, at 22,793 to 23,269
GFF7241
GFF7242 - hypothetical protein, at 23,286 to 23,660
GFF7242
Position (kb) 20
21
22
23 Strain fitness (log2 ratio) -2
-1
0
1
2 at 20.082 kb on + strand, within GFF7238 at 20.238 kb on + strand at 20.238 kb on + strand at 20.238 kb on + strand at 20.238 kb on + strand at 20.238 kb on + strand at 20.238 kb on + strand at 20.239 kb on - strand at 20.239 kb on - strand at 20.510 kb on + strand, within GFF7239 at 20.511 kb on - strand, within GFF7239 at 20.885 kb on + strand, within GFF7239 at 20.886 kb on - strand, within GFF7239 at 20.886 kb on - strand, within GFF7239 at 20.886 kb on - strand, within GFF7239 at 20.907 kb on + strand at 20.907 kb on + strand at 20.908 kb on - strand at 20.991 kb on + strand at 20.991 kb on + strand at 20.991 kb on + strand at 20.991 kb on + strand at 20.991 kb on + strand at 20.991 kb on + strand at 20.991 kb on + strand at 20.992 kb on - strand at 20.992 kb on - strand at 20.992 kb on - strand at 21.156 kb on + strand, within GFF7240 at 21.157 kb on - strand, within GFF7240 at 21.157 kb on - strand, within GFF7240 at 21.157 kb on - strand, within GFF7240 at 21.157 kb on - strand, within GFF7240 at 21.157 kb on - strand, within GFF7240 at 21.291 kb on + strand, within GFF7240 at 21.390 kb on + strand, within GFF7240 at 21.390 kb on + strand, within GFF7240 at 21.390 kb on + strand, within GFF7240 at 21.864 kb on + strand, within GFF7240 at 21.865 kb on - strand, within GFF7240 at 21.865 kb on - strand, within GFF7240 at 21.865 kb on - strand, within GFF7240 at 21.987 kb on + strand, within GFF7240 at 21.987 kb on + strand, within GFF7240 at 21.987 kb on + strand, within GFF7240 at 21.988 kb on - strand, within GFF7240 at 21.988 kb on - strand, within GFF7240 at 21.988 kb on - strand, within GFF7240 at 21.990 kb on + strand, within GFF7240 at 21.990 kb on + strand, within GFF7240 at 21.990 kb on + strand, within GFF7240 at 21.990 kb on + strand, within GFF7240 at 21.991 kb on - strand, within GFF7240 at 21.991 kb on - strand, within GFF7240 at 22.034 kb on + strand, within GFF7240 at 22.035 kb on - strand, within GFF7240 at 22.035 kb on - strand, within GFF7240 at 22.120 kb on - strand, within GFF7240 at 22.401 kb on + strand, within GFF7240 at 22.401 kb on + strand, within GFF7240 at 22.402 kb on - strand, within GFF7240 at 22.895 kb on + strand, within GFF7241 at 22.895 kb on + strand, within GFF7241 at 22.895 kb on + strand, within GFF7241 at 22.896 kb on - strand, within GFF7241 at 23.094 kb on - strand, within GFF7241 at 23.401 kb on + strand, within GFF7242
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 20,082 + GFF7238 0.68 +0.5 20,238 + -0.3 20,238 + +0.8 20,238 + -0.2 20,238 + +0.3 20,238 + -0.6 20,238 + +0.2 20,239 - +1.6 20,239 - -0.1 20,510 + GFF7239 0.24 +0.2 20,511 - GFF7239 0.24 -0.6 20,885 + GFF7239 0.88 +1.0 20,886 - GFF7239 0.88 +0.5 20,886 - GFF7239 0.88 -1.0 20,886 - GFF7239 0.88 -0.2 20,907 + -1.7 20,907 + -0.8 20,908 - -2.0 20,991 + -0.5 20,991 + +0.5 20,991 + -0.3 20,991 + +0.7 20,991 + -2.5 20,991 + +0.4 20,991 + -1.7 20,992 - +0.6 20,992 - -0.5 20,992 - -0.7 21,156 + GFF7240 0.11 +0.4 21,157 - GFF7240 0.11 +0.4 21,157 - GFF7240 0.11 +0.2 21,157 - GFF7240 0.11 +0.1 21,157 - GFF7240 0.11 -1.0 21,157 - GFF7240 0.11 +0.3 21,291 + GFF7240 0.19 -0.6 21,390 + GFF7240 0.25 -1.8 21,390 + GFF7240 0.25 +2.6 21,390 + GFF7240 0.25 -0.6 21,864 + GFF7240 0.53 -0.4 21,865 - GFF7240 0.54 -2.1 21,865 - GFF7240 0.54 +0.2 21,865 - GFF7240 0.54 -0.7 21,987 + GFF7240 0.61 -0.0 21,987 + GFF7240 0.61 +0.0 21,987 + GFF7240 0.61 -0.4 21,988 - GFF7240 0.61 -0.5 21,988 - GFF7240 0.61 +0.2 21,988 - GFF7240 0.61 +0.2 21,990 + GFF7240 0.61 +0.4 21,990 + GFF7240 0.61 +0.8 21,990 + GFF7240 0.61 +1.4 21,990 + GFF7240 0.61 -1.5 21,991 - GFF7240 0.61 +0.2 21,991 - GFF7240 0.61 -0.1 22,034 + GFF7240 0.64 -1.0 22,035 - GFF7240 0.64 -1.3 22,035 - GFF7240 0.64 +1.2 22,120 - GFF7240 0.69 -2.2 22,401 + GFF7240 0.86 -0.4 22,401 + GFF7240 0.86 +0.4 22,402 - GFF7240 0.86 +0.6 22,895 + GFF7241 0.21 -1.8 22,895 + GFF7241 0.21 -0.4 22,895 + GFF7241 0.21 -0.6 22,896 - GFF7241 0.22 +0.4 23,094 - GFF7241 0.63 +0.4 23,401 + GFF7242 0.31 -1.6
Or see this region's nucleotide sequence