Strain Fitness in Variovorax sp. SCN45 around GFF7228

Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF7226 and GFF7227 are separated by 7 nucleotidesGFF7227 and GFF7228 are separated by 175 nucleotidesGFF7228 and GFF7229 are separated by 28 nucleotides GFF7226 - hypothetical protein, at 7,496 to 8,335 GFF7226 GFF7227 - no description, at 8,343 to 8,612 GFF7227 GFF7228 - Ribonucleotide reductase of class II (coenzyme B12-dependent) (EC 1.17.4.1), at 8,788 to 10,638 GFF7228 GFF7229 - no description, at 10,667 to 11,926 GFF7229 Position (kb) 8 9 10 11Strain fitness (log2 ratio) -2 -1 0 1 2 3at 8.144 kb on + strand, within GFF7226at 8.144 kb on + strand, within GFF7226at 8.144 kb on + strand, within GFF7226at 8.145 kb on - strand, within GFF7226at 8.145 kb on - strand, within GFF7226at 8.145 kb on - strand, within GFF7226at 8.638 kb on + strandat 8.639 kb on - strandat 8.703 kb on + strandat 8.704 kb on - strandat 8.875 kb on + strandat 8.875 kb on + strandat 8.876 kb on - strandat 9.127 kb on + strand, within GFF7228at 9.127 kb on + strand, within GFF7228at 9.128 kb on - strand, within GFF7228at 9.128 kb on - strand, within GFF7228at 9.537 kb on - strand, within GFF7228at 9.538 kb on + strand, within GFF7228at 9.539 kb on - strand, within GFF7228at 10.078 kb on + strand, within GFF7228at 10.079 kb on - strand, within GFF7228at 10.291 kb on + strand, within GFF7228at 10.292 kb on - strand, within GFF7228at 10.427 kb on - strand, within GFF7228at 10.478 kb on - strandat 10.498 kb on + strandat 10.498 kb on + strandat 10.498 kb on + strandat 10.498 kb on + strandat 10.498 kb on + strandat 10.498 kb on + strandat 10.498 kb on + strandat 10.498 kb on + strandat 10.499 kb on - strandat 10.499 kb on - strandat 10.540 kb on + strandat 10.541 kb on - strandat 10.743 kb on + strandat 10.743 kb on + strandat 10.743 kb on + strandat 10.744 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
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8,144 + GFF7226 0.77 -0.4
8,144 + GFF7226 0.77 -1.8
8,144 + GFF7226 0.77 -2.0
8,145 - GFF7226 0.77 -0.5
8,145 - GFF7226 0.77 +0.5
8,145 - GFF7226 0.77 -0.6
8,638 + -1.3
8,639 - -0.2
8,703 + +2.4
8,704 - -1.0
8,875 + -1.0
8,875 + -0.9
8,876 - -1.0
9,127 + GFF7228 0.18 -0.8
9,127 + GFF7228 0.18 +0.3
9,128 - GFF7228 0.18 -1.0
9,128 - GFF7228 0.18 -0.0
9,537 - GFF7228 0.40 +0.6
9,538 + GFF7228 0.41 +1.2
9,539 - GFF7228 0.41 -0.2
10,078 + GFF7228 0.70 -0.4
10,079 - GFF7228 0.70 +1.0
10,291 + GFF7228 0.81 -0.8
10,292 - GFF7228 0.81 -0.2
10,427 - GFF7228 0.89 -0.6
10,478 - -0.5
10,498 + -1.2
10,498 + -0.2
10,498 + +0.4
10,498 + -0.3
10,498 + +0.2
10,498 + -1.3
10,498 + +0.5
10,498 + -0.7
10,499 - -0.3
10,499 - +3.0
10,540 + +1.6
10,541 - +0.4
10,743 + +0.8
10,743 + -1.2
10,743 + -0.0
10,744 - -0.3

Or see this region's nucleotide sequence