Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7199 and GFF7200 are separated by 16 nucleotides GFF7200 and GFF7201 overlap by 4 nucleotides GFF7201 and GFF7202 are separated by 17 nucleotides
GFF7199 - Alkanesulfonates-binding protein, at 17,481 to 18,575
GFF7199
GFF7200 - Alkanesulfonates transport system permease protein, at 18,592 to 19,440
GFF7200
GFF7201 - Alkanesulfonates transport system permease protein, at 19,437 to 20,252
GFF7201
GFF7202 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 20,270 to 21,142
GFF7202
Position (kb)
18
19
20 Strain fitness (log2 ratio)
-2
-1
0
1 at 17.860 kb on - strand, within GFF7199 at 17.860 kb on - strand, within GFF7199 at 18.120 kb on + strand, within GFF7199 at 18.121 kb on - strand, within GFF7199 at 18.121 kb on - strand, within GFF7199 at 18.475 kb on - strand at 18.513 kb on + strand at 18.513 kb on + strand at 18.513 kb on + strand at 18.949 kb on + strand, within GFF7200 at 18.949 kb on + strand, within GFF7200 at 18.950 kb on - strand, within GFF7200 at 18.950 kb on - strand, within GFF7200 at 18.950 kb on - strand, within GFF7200 at 19.045 kb on + strand, within GFF7200 at 19.046 kb on - strand, within GFF7200 at 19.144 kb on + strand, within GFF7200 at 19.144 kb on + strand, within GFF7200 at 19.144 kb on + strand, within GFF7200 at 19.144 kb on + strand, within GFF7200 at 19.144 kb on + strand, within GFF7200 at 19.144 kb on + strand, within GFF7200 at 19.144 kb on + strand, within GFF7200 at 19.145 kb on - strand, within GFF7200 at 19.145 kb on - strand, within GFF7200 at 19.145 kb on - strand, within GFF7200 at 19.145 kb on - strand, within GFF7200 at 19.145 kb on - strand, within GFF7200 at 19.151 kb on - strand, within GFF7200 at 19.291 kb on + strand, within GFF7200 at 19.292 kb on - strand, within GFF7200 at 20.032 kb on - strand, within GFF7201 at 20.046 kb on + strand, within GFF7201 at 20.047 kb on - strand, within GFF7201 at 20.333 kb on + strand at 20.333 kb on + strand at 20.333 kb on + strand at 20.333 kb on + strand at 20.334 kb on - strand at 20.334 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 17,860 - GFF7199 0.35 -0.2 17,860 - GFF7199 0.35 +0.0 18,120 + GFF7199 0.58 -1.2 18,121 - GFF7199 0.58 -0.4 18,121 - GFF7199 0.58 -2.3 18,475 - -0.8 18,513 + +0.7 18,513 + +0.1 18,513 + -0.3 18,949 + GFF7200 0.42 -0.6 18,949 + GFF7200 0.42 -0.2 18,950 - GFF7200 0.42 -0.2 18,950 - GFF7200 0.42 -0.7 18,950 - GFF7200 0.42 -0.8 19,045 + GFF7200 0.53 -0.2 19,046 - GFF7200 0.53 +0.2 19,144 + GFF7200 0.65 -0.7 19,144 + GFF7200 0.65 -1.0 19,144 + GFF7200 0.65 -1.0 19,144 + GFF7200 0.65 -1.1 19,144 + GFF7200 0.65 +0.3 19,144 + GFF7200 0.65 -0.9 19,144 + GFF7200 0.65 +0.0 19,145 - GFF7200 0.65 +0.5 19,145 - GFF7200 0.65 -1.0 19,145 - GFF7200 0.65 -1.1 19,145 - GFF7200 0.65 -0.3 19,145 - GFF7200 0.65 +0.5 19,151 - GFF7200 0.66 -1.3 19,291 + GFF7200 0.82 +1.1 19,292 - GFF7200 0.82 -0.4 20,032 - GFF7201 0.73 -0.9 20,046 + GFF7201 0.75 +0.3 20,047 - GFF7201 0.75 +1.7 20,333 + -0.5 20,333 + -0.9 20,333 + +0.9 20,333 + -0.3 20,334 - -0.4 20,334 - -0.5
Or see this region's nucleotide sequence