Experiment: M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7148 and GFF7149 are separated by 11 nucleotides GFF7149 and GFF7150 are separated by 14 nucleotides
GFF7148 - D-amino-acid oxidase (EC 1.4.3.3), at 13,636 to 14,739
GFF7148
GFF7149 - 4-hydroxyproline epimerase (EC 5.1.1.8), at 14,751 to 15,686
GFF7149
GFF7150 - Branched-chain amino acid ABC transporter, substrate-binding protein LivJ (TC 3.A.1.4.1), at 15,701 to 16,843
GFF7150
Position (kb) 14
15
16 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 13.886 kb on + strand, within GFF7148 at 13.886 kb on + strand, within GFF7148 at 13.887 kb on - strand, within GFF7148 at 13.887 kb on - strand, within GFF7148 at 14.048 kb on + strand, within GFF7148 at 14.048 kb on + strand, within GFF7148 at 14.048 kb on + strand, within GFF7148 at 14.048 kb on + strand, within GFF7148 at 14.048 kb on + strand, within GFF7148 at 14.049 kb on - strand, within GFF7148 at 14.049 kb on - strand, within GFF7148 at 14.049 kb on - strand, within GFF7148 at 14.312 kb on + strand, within GFF7148 at 14.312 kb on + strand, within GFF7148 at 14.312 kb on + strand, within GFF7148 at 14.312 kb on + strand, within GFF7148 at 14.312 kb on + strand, within GFF7148 at 14.312 kb on + strand, within GFF7148 at 14.312 kb on + strand, within GFF7148 at 14.312 kb on + strand, within GFF7148 at 14.312 kb on + strand, within GFF7148 at 14.312 kb on + strand, within GFF7148 at 14.313 kb on - strand, within GFF7148 at 14.313 kb on - strand, within GFF7148 at 14.313 kb on - strand, within GFF7148 at 14.313 kb on - strand, within GFF7148 at 14.504 kb on + strand, within GFF7148 at 14.504 kb on + strand, within GFF7148 at 14.505 kb on - strand, within GFF7148 at 14.505 kb on - strand, within GFF7148 at 14.505 kb on - strand, within GFF7148 at 14.505 kb on - strand, within GFF7148 at 14.505 kb on - strand, within GFF7148 at 14.866 kb on + strand, within GFF7149 at 14.866 kb on + strand, within GFF7149 at 14.866 kb on + strand, within GFF7149 at 14.867 kb on - strand, within GFF7149 at 14.867 kb on - strand, within GFF7149 at 14.867 kb on - strand, within GFF7149 at 14.867 kb on - strand, within GFF7149 at 14.992 kb on + strand, within GFF7149 at 14.993 kb on - strand, within GFF7149 at 14.993 kb on - strand, within GFF7149 at 14.993 kb on - strand, within GFF7149 at 15.133 kb on + strand, within GFF7149 at 15.133 kb on + strand, within GFF7149 at 15.134 kb on - strand, within GFF7149 at 15.134 kb on - strand, within GFF7149 at 15.134 kb on - strand, within GFF7149 at 15.277 kb on + strand, within GFF7149 at 15.437 kb on - strand, within GFF7149 at 15.437 kb on - strand, within GFF7149 at 15.437 kb on - strand, within GFF7149 at 15.437 kb on - strand, within GFF7149 at 15.705 kb on + strand at 15.705 kb on + strand at 15.705 kb on + strand at 15.705 kb on + strand at 15.705 kb on + strand at 15.705 kb on + strand at 15.705 kb on + strand at 15.705 kb on + strand at 15.705 kb on + strand at 15.705 kb on + strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.706 kb on - strand at 15.769 kb on + strand at 15.769 kb on + strand at 15.769 kb on + strand at 15.769 kb on + strand at 15.769 kb on + strand at 15.769 kb on + strand at 15.769 kb on + strand at 15.769 kb on + strand at 15.769 kb on + strand at 15.769 kb on + strand at 15.769 kb on + strand at 15.770 kb on - strand at 15.770 kb on - strand at 15.770 kb on - strand at 15.770 kb on - strand at 15.770 kb on - strand at 15.770 kb on - strand at 15.770 kb on - strand at 15.786 kb on + strand at 15.787 kb on - strand at 15.792 kb on + strand at 15.792 kb on + strand at 15.792 kb on + strand at 15.792 kb on + strand at 15.792 kb on + strand at 15.792 kb on + strand at 15.792 kb on + strand at 15.792 kb on + strand at 15.792 kb on + strand at 15.792 kb on + strand at 15.792 kb on + strand at 15.792 kb on + strand at 15.792 kb on + strand at 15.792 kb on + strand at 15.792 kb on + strand at 15.792 kb on + strand at 15.792 kb on + strand at 15.792 kb on + strand at 15.792 kb on + strand at 15.793 kb on - strand at 15.793 kb on - strand at 15.793 kb on - strand at 15.793 kb on - strand at 15.793 kb on - strand at 15.793 kb on - strand at 15.793 kb on - strand at 15.793 kb on - strand at 15.793 kb on - strand at 15.793 kb on - strand at 15.793 kb on - strand at 15.793 kb on - strand at 15.793 kb on - strand at 15.793 kb on - strand at 15.793 kb on - strand at 15.793 kb on - strand at 15.793 kb on - strand at 15.793 kb on - strand at 15.793 kb on - strand at 15.793 kb on - strand at 15.813 kb on + strand at 15.813 kb on + strand at 15.813 kb on + strand at 15.813 kb on + strand at 15.813 kb on + strand at 15.813 kb on + strand at 15.813 kb on + strand at 15.813 kb on + strand at 15.813 kb on + strand at 15.813 kb on + strand at 15.813 kb on + strand at 15.813 kb on + strand at 15.813 kb on + strand at 15.813 kb on + strand at 15.813 kb on + strand at 15.814 kb on - strand at 15.814 kb on - strand at 15.814 kb on - strand at 15.814 kb on - strand at 15.814 kb on - strand at 15.814 kb on - strand at 15.814 kb on - strand at 15.814 kb on - strand at 15.814 kb on - strand at 15.814 kb on - strand at 15.814 kb on - strand at 15.814 kb on - strand at 15.814 kb on - strand at 15.825 kb on + strand, within GFF7150 at 15.825 kb on + strand, within GFF7150 at 15.825 kb on + strand, within GFF7150 at 15.825 kb on + strand, within GFF7150 at 15.826 kb on - strand, within GFF7150 at 15.826 kb on - strand, within GFF7150 at 15.826 kb on - strand, within GFF7150 at 15.826 kb on - strand, within GFF7150 at 15.826 kb on - strand, within GFF7150 at 15.826 kb on - strand, within GFF7150 at 15.903 kb on + strand, within GFF7150 at 15.903 kb on + strand, within GFF7150 at 15.903 kb on + strand, within GFF7150 at 15.903 kb on + strand, within GFF7150 at 15.903 kb on + strand, within GFF7150 at 15.903 kb on + strand, within GFF7150 at 15.903 kb on + strand, within GFF7150 at 15.903 kb on + strand, within GFF7150 at 15.903 kb on + strand, within GFF7150 at 15.904 kb on - strand, within GFF7150 at 15.904 kb on - strand, within GFF7150 at 15.904 kb on - strand, within GFF7150 at 15.904 kb on - strand, within GFF7150 at 15.904 kb on - strand, within GFF7150 at 15.904 kb on - strand, within GFF7150 at 15.904 kb on - strand, within GFF7150 at 15.904 kb on - strand, within GFF7150 at 15.904 kb on - strand, within GFF7150 at 15.906 kb on + strand, within GFF7150 at 15.906 kb on + strand, within GFF7150 at 15.906 kb on + strand, within GFF7150 at 15.906 kb on + strand, within GFF7150 at 15.907 kb on - strand, within GFF7150 at 15.907 kb on - strand, within GFF7150 at 15.907 kb on - strand, within GFF7150 at 15.907 kb on - strand, within GFF7150 at 15.907 kb on - strand, within GFF7150 at 15.907 kb on - strand, within GFF7150 at 15.907 kb on - strand, within GFF7150 at 15.918 kb on + strand, within GFF7150 at 15.918 kb on + strand, within GFF7150 at 15.918 kb on + strand, within GFF7150 at 15.919 kb on - strand, within GFF7150 at 15.919 kb on - strand, within GFF7150 at 15.919 kb on - strand, within GFF7150 at 16.159 kb on - strand, within GFF7150 at 16.359 kb on + strand, within GFF7150 at 16.359 kb on + strand, within GFF7150 at 16.359 kb on + strand, within GFF7150 at 16.359 kb on + strand, within GFF7150 at 16.359 kb on + strand, within GFF7150 at 16.360 kb on - strand, within GFF7150 at 16.360 kb on - strand, within GFF7150 at 16.360 kb on - strand, within GFF7150 at 16.407 kb on + strand, within GFF7150 at 16.407 kb on + strand, within GFF7150 at 16.419 kb on + strand, within GFF7150 at 16.419 kb on + strand, within GFF7150 at 16.419 kb on + strand, within GFF7150 at 16.419 kb on + strand, within GFF7150 at 16.419 kb on + strand, within GFF7150 at 16.420 kb on - strand, within GFF7150 at 16.482 kb on + strand, within GFF7150 at 16.482 kb on + strand, within GFF7150 at 16.482 kb on + strand, within GFF7150 at 16.482 kb on + strand, within GFF7150 at 16.482 kb on + strand, within GFF7150 at 16.482 kb on + strand, within GFF7150 at 16.482 kb on + strand, within GFF7150 at 16.482 kb on + strand, within GFF7150 at 16.483 kb on - strand, within GFF7150 at 16.483 kb on - strand, within GFF7150 at 16.483 kb on - strand, within GFF7150 at 16.518 kb on + strand, within GFF7150 at 16.519 kb on - strand, within GFF7150 at 16.519 kb on - strand, within GFF7150
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and thiamine HCl 10 mg/mL remove 13,886 + GFF7148 0.23 +0.8 13,886 + GFF7148 0.23 -0.9 13,887 - GFF7148 0.23 -0.1 13,887 - GFF7148 0.23 -0.2 14,048 + GFF7148 0.37 -0.2 14,048 + GFF7148 0.37 -0.9 14,048 + GFF7148 0.37 -0.4 14,048 + GFF7148 0.37 -0.4 14,048 + GFF7148 0.37 -0.8 14,049 - GFF7148 0.37 -1.2 14,049 - GFF7148 0.37 -1.7 14,049 - GFF7148 0.37 -0.8 14,312 + GFF7148 0.61 +0.3 14,312 + GFF7148 0.61 +1.5 14,312 + GFF7148 0.61 -0.9 14,312 + GFF7148 0.61 -1.7 14,312 + GFF7148 0.61 +0.8 14,312 + GFF7148 0.61 -1.8 14,312 + GFF7148 0.61 -0.2 14,312 + GFF7148 0.61 -0.1 14,312 + GFF7148 0.61 +0.5 14,312 + GFF7148 0.61 -0.2 14,313 - GFF7148 0.61 -0.2 14,313 - GFF7148 0.61 -0.2 14,313 - GFF7148 0.61 +0.1 14,313 - GFF7148 0.61 -0.6 14,504 + GFF7148 0.79 -0.7 14,504 + GFF7148 0.79 -0.1 14,505 - GFF7148 0.79 -1.2 14,505 - GFF7148 0.79 -0.8 14,505 - GFF7148 0.79 -0.7 14,505 - GFF7148 0.79 +0.0 14,505 - GFF7148 0.79 -0.1 14,866 + GFF7149 0.12 -1.0 14,866 + GFF7149 0.12 -0.4 14,866 + GFF7149 0.12 -1.2 14,867 - GFF7149 0.12 +0.2 14,867 - GFF7149 0.12 +1.1 14,867 - GFF7149 0.12 +0.2 14,867 - GFF7149 0.12 +0.6 14,992 + GFF7149 0.26 +0.9 14,993 - GFF7149 0.26 -0.4 14,993 - GFF7149 0.26 -0.7 14,993 - GFF7149 0.26 +0.0 15,133 + GFF7149 0.41 -1.9 15,133 + GFF7149 0.41 +0.6 15,134 - GFF7149 0.41 -0.8 15,134 - GFF7149 0.41 +0.3 15,134 - GFF7149 0.41 -0.8 15,277 + GFF7149 0.56 -1.2 15,437 - GFF7149 0.73 -1.0 15,437 - GFF7149 0.73 +1.3 15,437 - GFF7149 0.73 +0.5 15,437 - GFF7149 0.73 +0.7 15,705 + -2.2 15,705 + +0.8 15,705 + -1.6 15,705 + -0.4 15,705 + -0.1 15,705 + +0.3 15,705 + +0.1 15,705 + -0.9 15,705 + +0.9 15,705 + -0.4 15,706 - -1.8 15,706 - -0.7 15,706 - -3.0 15,706 - -1.1 15,706 - -0.7 15,706 - +0.8 15,706 - -1.4 15,706 - -1.0 15,706 - -1.3 15,706 - +0.2 15,706 - -0.3 15,706 - +0.2 15,706 - -0.2 15,769 + -1.4 15,769 + -0.5 15,769 + -0.7 15,769 + -0.1 15,769 + +0.8 15,769 + -0.3 15,769 + +1.1 15,769 + -0.7 15,769 + +0.4 15,769 + +0.3 15,769 + -1.3 15,770 - +0.5 15,770 - -1.2 15,770 - +0.8 15,770 - -0.1 15,770 - +0.4 15,770 - -0.3 15,770 - -0.2 15,786 + -1.1 15,787 - +1.8 15,792 + +0.0 15,792 + +0.5 15,792 + +1.3 15,792 + -0.3 15,792 + +0.7 15,792 + -0.4 15,792 + -0.3 15,792 + -0.4 15,792 + -0.8 15,792 + +2.3 15,792 + +0.8 15,792 + -0.1 15,792 + +0.5 15,792 + +1.6 15,792 + -0.5 15,792 + -0.1 15,792 + -0.2 15,792 + -0.5 15,792 + -0.6 15,793 - -0.7 15,793 - -0.4 15,793 - -0.7 15,793 - -0.4 15,793 - -0.5 15,793 - -0.2 15,793 - +0.1 15,793 - +0.3 15,793 - -0.2 15,793 - -0.7 15,793 - +0.8 15,793 - +0.1 15,793 - -0.3 15,793 - -1.8 15,793 - +0.5 15,793 - -1.4 15,793 - +0.4 15,793 - -1.0 15,793 - +1.1 15,793 - +0.1 15,813 + -0.8 15,813 + -1.0 15,813 + -0.8 15,813 + +0.1 15,813 + -1.1 15,813 + -1.1 15,813 + +1.4 15,813 + -2.4 15,813 + +1.2 15,813 + -0.3 15,813 + -1.1 15,813 + -1.4 15,813 + -0.3 15,813 + -0.7 15,813 + -0.4 15,814 - +0.8 15,814 - -0.4 15,814 - +0.0 15,814 - +1.3 15,814 - +2.0 15,814 - -0.6 15,814 - -0.4 15,814 - -1.0 15,814 - -0.2 15,814 - -0.5 15,814 - +0.3 15,814 - +0.4 15,814 - +0.0 15,825 + GFF7150 0.11 +0.1 15,825 + GFF7150 0.11 -0.4 15,825 + GFF7150 0.11 -0.7 15,825 + GFF7150 0.11 -1.5 15,826 - GFF7150 0.11 -0.1 15,826 - GFF7150 0.11 -0.2 15,826 - GFF7150 0.11 -0.2 15,826 - GFF7150 0.11 -0.2 15,826 - GFF7150 0.11 -0.0 15,826 - GFF7150 0.11 -0.7 15,903 + GFF7150 0.18 -1.8 15,903 + GFF7150 0.18 +0.8 15,903 + GFF7150 0.18 -0.2 15,903 + GFF7150 0.18 -0.6 15,903 + GFF7150 0.18 -0.6 15,903 + GFF7150 0.18 -1.4 15,903 + GFF7150 0.18 -0.4 15,903 + GFF7150 0.18 -0.4 15,903 + GFF7150 0.18 +0.8 15,904 - GFF7150 0.18 +0.3 15,904 - GFF7150 0.18 +0.4 15,904 - GFF7150 0.18 +1.1 15,904 - GFF7150 0.18 -1.1 15,904 - GFF7150 0.18 +0.1 15,904 - GFF7150 0.18 -0.0 15,904 - GFF7150 0.18 -1.4 15,904 - GFF7150 0.18 -2.5 15,904 - GFF7150 0.18 +0.5 15,906 + GFF7150 0.18 +0.3 15,906 + GFF7150 0.18 +0.4 15,906 + GFF7150 0.18 -0.9 15,906 + GFF7150 0.18 -1.6 15,907 - GFF7150 0.18 -0.2 15,907 - GFF7150 0.18 -0.4 15,907 - GFF7150 0.18 -0.1 15,907 - GFF7150 0.18 -0.1 15,907 - GFF7150 0.18 +1.3 15,907 - GFF7150 0.18 -0.6 15,907 - GFF7150 0.18 +0.3 15,918 + GFF7150 0.19 -0.9 15,918 + GFF7150 0.19 +0.0 15,918 + GFF7150 0.19 +0.6 15,919 - GFF7150 0.19 -1.8 15,919 - GFF7150 0.19 +0.3 15,919 - GFF7150 0.19 -1.4 16,159 - GFF7150 0.40 -0.9 16,359 + GFF7150 0.58 -1.1 16,359 + GFF7150 0.58 -0.2 16,359 + GFF7150 0.58 +0.0 16,359 + GFF7150 0.58 -1.5 16,359 + GFF7150 0.58 +0.0 16,360 - GFF7150 0.58 -1.4 16,360 - GFF7150 0.58 +0.4 16,360 - GFF7150 0.58 -0.5 16,407 + GFF7150 0.62 -0.7 16,407 + GFF7150 0.62 -0.7 16,419 + GFF7150 0.63 -1.1 16,419 + GFF7150 0.63 +0.8 16,419 + GFF7150 0.63 -0.3 16,419 + GFF7150 0.63 +1.7 16,419 + GFF7150 0.63 -1.6 16,420 - GFF7150 0.63 -1.3 16,482 + GFF7150 0.68 +0.3 16,482 + GFF7150 0.68 +1.1 16,482 + GFF7150 0.68 -0.5 16,482 + GFF7150 0.68 -1.7 16,482 + GFF7150 0.68 +0.8 16,482 + GFF7150 0.68 -0.9 16,482 + GFF7150 0.68 +0.2 16,482 + GFF7150 0.68 -0.8 16,483 - GFF7150 0.68 +0.5 16,483 - GFF7150 0.68 -1.1 16,483 - GFF7150 0.68 +2.3 16,518 + GFF7150 0.71 +0.8 16,519 - GFF7150 0.72 +1.0 16,519 - GFF7150 0.72 +0.3
Or see this region's nucleotide sequence